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Puregene Accessories

从各种类型的样本中纯化可存档的DNA

  • 高品质DNA用于长期储存
  • 高重复性的DNA纯化
  • 方便、可扩展的纯化操作

Puregene Accessories配合Gentra Puregene Kits纯化高分子量(100–200 kb)DNA,适合用于存档。可扩展的纯化操作,能温和的去除大体积样本中的污染物和抑制剂,纯化获得的大体积样本适合长期保存。

Cat No./ID: 158902
RBC Lysis Solution (450 ml)
450 ml RBC Lysis Solution
Cat No./ID: 158904
RBC Lysis Solution (1000 ml)
1000 ml RBC Lysis Solution
Cat No./ID: 158906
Cell Lysis Solution (125 ml)
125 ml Cell Lysis Solution
Cat No./ID: 158908
Cell Lysis Solution (1000 ml)
1000 ml Cell Lysis Solution
Cat No./ID: 158910
Protein Precipitation Solution (50 ml)
50 ml Protein Precipitation Solution
Cat No./ID: 158912
Protein Precipitation Solution (350 ml)
350 ml Protein Precipitation Solution
Cat No./ID: 158914
DNA Hydration Solution (100 ml)
100 ml DNA Hydration Solution
Cat No./ID: 158916
DNA Hydration Solution (500 ml)
500 ml DNA Hydration Solution
Cat No./ID: 158918
Puregene Proteinase K (650 µl)
650 µl Puregene Proteinase K
Cat No./ID: 158920
Puregene Proteinase K (5 ml)
5 ml Puregene Proteinase K
Cat No./ID: 158922
RNase A Solution (650 µl)
650 µl RNase A Solution
Cat No./ID: 158924
RNase A Solution (5 ml)
5 ml RNase A Solution
Cat No./ID: 158926
Cell Suspension Sol. (125 ml)
Cell Suspension Sol. (125 ml)
Cat No./ID: 158928
Lytic Enzyme Solution (650 µl)
650 µl Lytic Enzyme Solution
Cat No./ID: 158930
Glycogen Solution (500 µl)
500 µl Glycogen Solution
Cat No./ID: 158932
Clotspin Baskets (50)
50 Clotspin Baskets
Principle
应用Gentra Puregene Kits,细胞用阴离子洗涤剂裂解,其中加入DNA稳定剂。DNA稳定剂限制胞内及其他环境中的DNases的活性。使用RNA降解酶去除RNA。蛋白质等其他污染物经盐析沉淀法去除。最终,通过乙醇沉淀回收基因组DNA,水合溶液(1 mM EDTA、10 mM Tris·Cl pH 7.5)溶解DNA。一般情况下,纯化的DNA的A260/A280值在1.7–1.9之间,大小可达200 kb。纯化的DNA可贮存于2–8°C、–20°C或–80°C,确保安全。
Procedure
可存档的DNA  

DNA的纯度对下游应用结果的精确性至关重要,诸如PCR等敏感的下游应用要求使用最高品质、且分子量尽可能大的DNA,以确保可靠的结果。Gentra Puregene Kits去除污染物和酶抑制剂,确保获得高度稳定的适合存档的DNA。纯化获得高品质的DNA,分子大小为100–200 kb。

Applications

应用Puregene Accessories和Gentra Puregene Kits纯化的DNA高度稳定,适用于各种下游应用,包括:

DNA存档
PCR
SNP分析
Southern印迹

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For purification of archive-quality DNA from human whole blood, bone marrow, buffy coat, buccal cells, body fluids, cultured cells, tissue, mouse tail, yeast, bacteria
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