低通量样本破碎,适用于分子分析

  • 快速、高效的破碎各种类型的样本
  • 一次性钻头有助于消除交叉污染
  • 一次性钻头节省实验时间
  • 透明钻头确保破碎过程可监控
  • 与QIAGEN样本制备技术无缝整合

TissueRuptor是一款手持式匀浆器,可快速、有效、灵活的对人类、动物和植物样本进行破碎,适用于多种下游应用。TissueRuptor使用透明的一次性钻头,可避免交叉污染,并且可以直观的监控样本的破碎过程。此外,使用一次性钻头可以节省每次处理样本之后的清洁时间。

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TissueRuptor II (230 V, 50/60 Hz, EU/CH)
Handheld rotor–stator homogenizer, 230 V, 50/60 Hz (for Europe [excluding UK and Ireland]), 5 TissueRuptor Disposable Probes
9002756
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TissueRuptor Disposable Probes (25)
25 nonsterile plastic disposable probes for use with the TissueRuptor II (and the old TissueRuptor)
990890
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The TissueRuptor is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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Reproducible purification of high-quality genomic DNA.|Efficient disruption and homogenization of animal tissues.|Successful purification of phosphoproteins.|High performance results in PCR.|Pure RNA with high RIN values.|
Frozen liver and kidney samples (30 mg each) were disrupted in lysis buffer at full speed for 30 seconds using the TissueRuptor. Genomic DNA was purified using the AllPrep DNA/RNA Mini Kit and analyzed by agarose gel electrophoresis. M: markers.|The following tissues were disrupted at full speed for 30 seconds using the TissueRuptor: frozen kidney, liver, and spleen (10 mg each); frozen lung (250 mg); stabilized heart, muscle, and brain (10 mg each). Samples were either disrupted in lysis buffer (kidney, liver, spleen, lung, heart, and muscle) or QIAzol Lysis Reagent (brain). Total RNA was purified using the RNeasy Plus Mini Kit (RNeasy Plus), the RNeasy Midi Kit (RNeasy Midi), the RNeasy Fibrous Tissue Mini Kit (RNeasy Fibrous), or the RNeasy Lipid Tissue Mini Kit (RNeasy Lipid). Purified RNA was analyzed by formaldehyde agarose gel electrophoresis. The gels show the high yields and quality of the RNA following disruption using the TissueRuptor.|Frozen rat liver and brain (approximately 30 mg each) were disrupted at medium speed for 30–60 seconds in 350 μl PhosphoProtein Lysis Buffer. The homogenized samples were then diluted with 1500 μl PhosphoProtein Lysis Buffer and incubated at 4°C for 30 minutes, with brief vortexing every 10 minutes. Phosphoproteins were purified using the PhosphoProtein Purification Kit and detected by western blotting. [A] Transfer membrane stained with Ponceau S, and [B] western blot. M: markers; FT: flow-through; E3: elution fraction 3.|[A] Liver and [B] heart samples (25 mg each, frozen) were disrupted with the TissueRuptor or a traditional rotor–stator homogenizer and lysed for 1 hour with proteinase K, or lysed overnight with proteinase K without sample disruption. DNA was purified using the QIAamp DNA Mini Kit. Eluates (5 μl) were subjected to PCR using HotStarTaq DNA Polymerase and primers specific for the 18S ribosomal RNA gene. M: GelPilot Mid Range Ladder.|Frozen liver samples (30 mg each) were disrupted at full speed for 30 seconds using either the TissueRuptor with disposable probes or a traditional rotor–stator homogenizer with a steel generator probe. Total RNA was purified using the RNeasy Plus Mini Kit and analyzed on the Agilent 2100 Bioanalyzer. The high RNA Integrity Number (RIN) indicates the high quality of the RNA.|
Performance
QIAGEN已验证表明TissueRuptor可与QIAGEN样本制备试剂盒配合使用。使用TissueRuptor进行样本破碎确保获得最大产量的核酸和蛋白质,用于PCR、Western印迹和酶分析等下游应用(参见"Successful purification of phosphoprotein"和"High performance results in PCR")。对于多数组织,使用TissueRuptor进行破碎和匀浆达到的效果与传统转子定子式匀浆器的效果相当(参见"Pure RNA with high RIN values")。TissueRuptor可实现对组织样本的高效破碎,因此使用QIAGEN核酸纯化试剂盒可持续获得高品质DNA和RNA(参见"Pure RNA with high RIN values"、"Reproducible purification of high-quality genomic DNA"和"Efficient disruption and homogenization of animal tissues")。
Principle

基因分型、基因表达分析和蛋白质组学研究均要求对生物样本进行高效破碎,以确保获得高产的DNA、RNA和蛋白质。TissueRuptor可快速、彻底的破碎样本,彻底释放生物分子,并可同时对样本进行匀质化,协助后续使用QIAGEN试剂盒进行纯化。

QIAGEN提供从样本收集到DNA、RNA和蛋白质纯化的完整解决方案,TissueRuptor是其中关键的组成部分。优化的实验方案整合了样本破碎和生物分子纯化,提供高效的工作流程。此外,多种自动化解决方案可用于低至高通量的生物分子纯化和分析。

QIAGEN的RNAlater RNA Stabilization Reagent可稳定RNA,Allprotect Tissue Reagent可稳定DNA、RNA和蛋白质。TissueRuptor可轻松破碎使用以上试剂采集的组织样本。

<>与QIAGEN破碎仪器兼容的QIAGEN纯化试剂盒
纯化的待分析物 样本类型 QIAGEN试剂盒
RNA 容易裂解的组织 RNeasy Kits
RNeasy Plus Kits
RNeasy Protect Kits
RNA 富含纤维的组织 RNeasy Fibrous Tissue Kits
RNA 各种类型组织 RNeasy Lipid Tissue Kits
RNeasy Universal Tissue Kits
RNA 植物组织 RNeasy Plant Mini Kit
RNA 酵母 RNeasy Kits
RNA 细菌 RNeasy Protect Bacteria Kits
microRNA 各种类型组织 miRNeasy Kits
DNA 人类组织 QIAamp DNA Kits
DNA 动物组织 DNeasy Blood & Tissue Kits
蛋白质 组织 Qproteome Mammalian Protein Prep Kit
磷蛋白 组织 PhosphoProtein Purification Kit
糖蛋白 组织 Qproteome Glycoprotein Kits
DNA和RNA 组织 AllPrep DNA/RNA Kits
DNA、RNA和蛋白质 组织 AllPrep DNA/RNA/Protein Mini Kit
Procedure

TissueRuptor是一款手持式设备,带有TissueRuptor一次性钻头。钻头的刀片高速旋转,通过搅动和机械剪切作用,同时破碎和匀质化样本。适用的样本类型包括人类、动物和植物组织(对于难破碎的植物组织,建议使用TissueLyser II)。通常只需全速破碎30秒,即可释放起始样本中的核酸或蛋白质。

样本破碎完成后,可丢弃TissueRuptor的一次性钻头,使用新钻头破碎下一个样本。无需清洗、再使用同一钻头,因此可节省时间,避免交叉污染。此外,TissueRuptor使用一次性透明钻头,可以直观的监控样本破碎过程。

Applications

TissueRuptor可高效破碎并匀浆人类、植物和动物组织样本,包括血块。使用QIAGEN试剂盒对RNA、DNA、总核酸或蛋白质进行纯化。

Feature
Specifications
Disruption principle Simultaneous disruption and homogenization by rotation of the blade of the disposable probe
Features Rapid, effective disruption of a range of sample types. Disposable probes minimize risk of cross-contamination. Time savings through use of disposable probes. Visual monitoring of disruption using transparent probes. Seamless integration with QIAGEN sample technologies.
Kits compatible with instrument All Kits for purification of RNA/DNA/protein
Technical data EU / UK : 230V AC 125W 10,000 – 33,000rpm; US:120V ~ 1.15A 60Hz 5,000 – 35,000rpm
Technology Handheld rotor-stator homogenizer
Throughput 1 sample per run
Typical run time 15–120 seconds

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Kit Handbooks
2
For low-throughput disruption of biological samples using disposable probes
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