用于低到中通量样本破碎,适用于分子分析

  • 同时破碎至多12个样本
  • 仪器结构紧凑
  • 配合可冷却的适配器,防止生物分子降解
  • 可处理各种类型的样本,获得高重复性结果
  • 与很多实验室流程兼容

TissueLyser LT是一款小型球磨仪,可同时快速、高效的破碎至多12个样品。该通量与自动化样品制备工作站QIAcube全自动核酸纯化仪一致,可配合值得信赖的QIAGEN离心柱试剂盒使用。用不锈钢珠或玻璃珠高速震荡放置于2 ml微型离心管中的样品,实现同步破碎和匀浆。TissueLyser LT必须配合可冷却的适配器TissueLyser LT Adapter使用,该适配器在破碎过程中可固定试管。其他附件包括研磨珠和研磨珠分配器。

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TissueLyser LT
Compact bead mill, 100–240 V AC, 50–60 Hz; requires the TissueLyser LT Adapter, 12-Tube (available separately)
85600
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TissueLyser LT Adapter, 12-Tube
Adapter for disruption of up to 12 samples in 2 ml microcentrifuge tubes on the TissueLyser LT
69980
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The TissueLyser LT is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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Effective tissue disruption.|Intact protein suitable for all types of analysis.|High-quality DNA from animal tissues.|High-quality DNA from plant tissues.|Intact RNA from plant tissues.|Pure RNA with high RIN values.|TissueLyser LT.|
Various rat tissues were disrupted using the TissueLyser LT or TissueLyser II. [A] DNA was purified from 25 mg samples on the QIAcube using the DNeasy Blood & Tissue Kit. DNA yields were determined using a spectrophotometer. [B] RNA was purified from 20 mg samples on the QIAcube using the RNeasy Fibrous Tissue Mini Kit (skin, heart, and lung) or RNeasy Lipid Tissue Mini Kit (brain). RNA yields were determined using a spectrophotometer.|Various rat tissues (25 mg each) stabilized in Allprotect Tissue Reagent were disrupted using the TissueLyser LT or TissueLyser II. Total protein was purified using the Qproteome Mammalian Protein Prep Kit and then analyzed by western blotting. [A] Transfer membrane stained with Ponceau S, and [B] western blot. LT: TissueLyser LT; II: TissueLyser II; M: markers.|Various rat tissues (25 mg each) stabilized in Allprotect Tissue Reagent were disrupted using the TissueLyser LT or TissueLyser II. DNA was purified on the QIAcube using the DNeasy Blood & Tissue Kit, and then used in PCR with the HotStarTaq Plus Master Mix Kit and a PGK1 primer system. The 120 bp PCR product was analyzed on the QIAxcel using the QIAxcel DNA High Resolution Kit. LT: TissueLyser LT; II: TissueLyser II; M: markers.|Various plant tissues (100 mg each) were disrupted in precooled adapters using the TissueLyser LT or TissueLyser II. DNA was purified on on the QIAcube using the DNeasy Plant Mini Kit and then analyzed on an agarose gel. LT: TissueLyser LT; II: TissueLyser II; M: markers.|Various plant tissues (100 mg each) were disrupted in duplicate in precooled adapters using the TissueLyser LT. RNA was purified using the RNeasy Plant Mini Kit and then analyzed on an agarose gel. The sharp ribosomal RNA bands indicate purification of intact RNA.|Rat heart and lung (20 mg each) were disrupted using the TissueLyser LT. RNA was purified on the QIAcube using the RNeasy Fibrous Tissue Mini Kit and then analyzed on the Agilent 2100 Bioanalyzer. The RIN values were 8.3 (heart) and 9 (lung).||
Performance

TissueLyser LT可高效破碎人类、动物和植物组织、细菌和酵母,确保后续DNA、RNA和蛋白质纯化中产量重复性好(参见"Effective tissue disruption")。心脏和大脑等难裂解组织,也可使用TissueLyser LT处理。破碎之后DNA、RNA和蛋白质保持完整(参见"Pure RNA with high RIN values"、"Intact protein suitable for all types of analysis"、"High-quality DNA from animal tissues"、"High-quality DNA from plant tissues"和"Intact RNA from plant tissues"),确保下游应用中可靠的分析。

TissueLyser LT的破碎效果与TissueLyser II相当(参见"Effective tissue disruption"、"Intact protein suitable for all types of analysis"、"High-quality DNA from animal tissues"和"High-quality DNA from plant tissues")。

Principle
 

基因分型、基因表达分析和蛋白质组学研究均要求对生物样本进行高效破碎,以确保获得高产的DNA、RNA和蛋白质。TissueLyser LT可快速、彻底的破碎样本,彻底释放生物分子,并可同时对样本进行匀质化,协助后续使用的QIAGEN试剂盒进行纯化(参见下表)。

QIAGEN提供从样本收集到DNA、RNA和蛋白质纯化的完整解决方案,TissueLyser LT是其中关键的组成部分。TissueLyser LT适用于QIAGEN手动样本制备试剂盒(参见下表),也是QIAGEN中通量样本制备和分析自动化解决方案的补充(参见下表)。包括应用QIAGEN离心柱试剂盒自动纯化样品的QIAcube全自动核酸纯化仪,以及对DNA和RNA进行毛细管凝胶电泳的QIAxcel。

QIAGEN纯化试剂盒配合QIAGEN破碎体系
纯化的分析物 样本类型 QIAGEN试剂盒
RNA 容易裂解的组织 RNeasy Kits
RNeasy Plus Kits
RNeasy Protect Kits
RNA 富含纤维的组织 RNeasy Fibrous Tissue Kits
RNA 各种类型的组织 RNeasy Lipid Tissue Kits
RNeasy Universal Tissue Kits
RNA 植物组织 RNeasy Plant Mini Kit
RNA 酵母 RNeasy Kits
RNA 细菌 RNeasy Protect Bacteria Kits
microRNA 各种类型的组织 miRNeasy Kits
DNA 人类组织 QIAamp DNA Kits
DNA 动物组织 DNeasy Blood & Tissue Kits
蛋白质 组织 Qproteome Mammalian Protein Prep Kit
磷蛋白 组织 PhosphoProtein Purification Kit
糖蛋白 组织 Qproteome Glycoprotein Kits
DNA和RNA 组织 AllPrep DNA/RNA Kits
DNA、RNA和蛋白质 组织 AllPrep DNA/RNA/Protein Mini Kit
QIAGEN medium-throughput automation
仪器 目的 通量
QIAcube 纯化DNA、RNA和蛋白质 每批多达12个样本
EZ1 Advanced 从人类样本中纯化DNA和RNA 每批多达6个样本
EZ1 Advanced XL 从人类样本中纯化DNA和RNA 每批多达14个样本
QIAxcel 对DNA片段和RNA进行电泳分析 每批多达96个样本
QIAgility 反应体系构建 每批多达384个样本
Rotor-Gene Q real-time PCR和HRM分析 每批多达100个样本
PyroMark Q24 甲基化和突变分析 每批多达24个样本
Procedure

TissueLyser LT与TissueLyser LT Adapter配合使用。每次操作可将多达12个2 ml的试管放置于适配器上,每管含一个样本和一颗研磨珠。适配器牢固的固定在TissueLyser LT的活塞上。活塞快速上下移动,使得研磨珠震动、研磨,可同时破碎并匀质化样本。

TissueLyser LT的大小只有15 cm x 27 cm,可放置于任何实验室。由于每个样本都安全的密封在试管内,可用TissueLyser LT同时纯化多个样本,而无交叉污染的风险。破碎新鲜或冷冻样品,可用干冰预先冷却TissueLyser LT Adapter,避免核酸和蛋白质降解(参见"High-quality DNA from plant tissues"和"Intact RNA from plant tissues")。组织可储存于 Allprotect Tissue Reagent(稳定DNA、RNA和蛋白质)或储存于RNAlater RNA Stabilization Reagent(稳定RNA),无需预先冷却适配器。

Applications

TissueLyser LT每批可处理多达12个样本,因此是遗传学、转录组学和蛋白质组学研究中获得生物信息的理想解决方案。TissueLyser LT可快速、统一的破碎动物和人类组织、植物组织、细菌和真菌,需与TissueLyser LT Adapter配合使用,TissueLyser LT Adapter可固定12个2 ml的样本管。样本纯化可以手动进行,也可以在QIAcube全自动核酸纯化仪、QIAsymphony SP核酸提取纯化分析仪、QIAxtractor、EZ1 Advanced、EZ1 Advanced XL、BioRobot或BioSprint工作站上自动进行。

Feature
Specifications
Dimensions Weight: Approx. 7 kg, Width: 150 mm, Depth: 270 mm, Height: 280 mm, The power supply of the TissueLyser LT is compatible with voltages of 100–240 V
Disruption principle High-speed shaking of samples in 2 ml microcentrifuge tubes with stainless steel or glass beads
Features Simultaneous disruption of up to 12 samples. Compact instrument with small footprint. Coolable adapter to prevent biomolecule degradation. Reproducible results with all sample types. Compatible with all laboratory workflows.
Kits compatible with instrument All kits for purification of RNA/DNA/protein
Protocol/main application on this intrument Sample preparation/sample disruption
Technology Bead Mill
Typical run time 40 sec - 5 min, depending on protocol

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Kit Handbooks
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For disruption of up to 12 samples on the TissueLyser LT
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For disruption of up to 12 biological samples
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