用于中到高通量样本破碎,适用于分子分析

  • 破碎过程便利、安全
  • 适配器专为高通量破碎而优化
  • 提供多种配件
  • 确保难裂解组织获得高重复性结果
  • QIAGEN样本处理体系的重要组成部分

通过不锈钢珠、碳化钨珠或玻璃珠在塑料样品管中的高速震动,TissueLyser II可同步破碎多个生物样本。借助适当的适配器,可同时处理48或192个样本。也可使用研磨罐处理大体积样本。同时提供各种研磨珠、研磨珠分配器、收集管及管盖。TissueLyser II包括适配器在内的所有配件与TissueLyser(已停产)兼容。

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TissueLyser II
Bead mill, 100–120/220–240 V, 50/60 Hz; requires the TissueLyser Adapter Set 2 x 24 or TissueLyser Adapter Set 2 x 96 (available separately)
85300
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The TissueLyser II is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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Reliable detection of miRNAs.|Efficient disruption of animal tissues.|Reproducible RNA purification from plant tissues.|Intact protein suitable for all types of analysis.|High-quality DNA from animal tissues.|High-quality DNA from plant tissues.|TissueLyser II.|
Rat kidney was stabilized in RNAlater RNA Stabilization Reagent, and 25 mg samples were disrupted in QIAzol Lysis Reagent for 2 x 5 minutes at 25 Hz using the TissueLyser II. Total RNA containing miRNA was purified using the miRNeasy Mini Kit, using either the manual procedure (Manual) or the automated procedure with the QIAcube (QIAcube). Real-time RT-PCR using the miScript System was carried out to detect 2 different miRNAs, miR-16 and miR-25.|Tissues (20 mg) were either frozen (Frozen) or stabilized in RNAlater RNA Stabilization Reagent (RNAlater), and then disrupted using the TissueLyser II (2 x 2 minutes for liver and muscle; 2 x 5 minutes for skin). RNA was purified using the RNeasy Mini Kit (liver), RNeasy Fibrous Tissue Mini Kit (skin), or RNeasy Lipid Tissue Mini Kit (muscle). [A] Analysis on a 1.2 % formaldehyde agarose gel shows sharp ribosomal RNA bands, indicating intact RNA. [B] RNA yields were quantified by A260 nm absorbance measurements.|Frozen plant leaves were disrupted using the TissueLyser II (2 x 1 minute). RNA was purified using the RNeasy Plant Mini Kit and analyzed on a 1.2 % formaldehyde agarose gel. The ribosomal RNA bands were sharp and of equal intensity, indicating reproducible purification of intact RNA. T: tomato (100 mg); A: arabidopsis (25 mg); C: cotton (100 mg); M: maize (100 mg); R: rape (100 mg).|Various rat tissues (25 mg each) stabilized in Allprotect Tissue Reagent were disrupted using the TissueLyser LT or TissueLyser II. Total protein was purified using the Qproteome Mammalian Protein Prep Kit and then analyzed by western blotting. Transfer membrane stained with Ponceau S, and western blot. LT: TissueLyser LT; II: TissueLyser II; M: markers.|Various rat tissues (25 mg each) stabilized in Allprotect Tissue Reagent were disrupted using the TissueLyser LT or TissueLyser II. DNA was purified on the QIAcube using the DNeasy Blood & Tissue Kit, and then used in PCR with the HotStarTaq Plus Master Mix Kit and a PGK1 primer system. The 120 bp PCR product was analyzed on the QIAxcel using the QIAxcel DNA High Resolution Kit. LT: TissueLyser LT; II: TissueLyser II; M: markers.|Various plant tissues (100 mg each) were disrupted in precooled adapters using the TissueLyser LT or TissueLyser II. DNA was purified on on the QIAcube using the DNeasy Plant Mini Kit and then analyzed on an agarose gel. LT: TissueLyser LT; II: TissueLyser II; M: markers.||
Performance
TissueLyser II尤其适合高通量的样本破碎,适用于人类、动物和植物组织、细菌和酵母。可纯化获得高品质的DNA、RNA、miRNA和蛋白质,甚至对于难裂解的组织也具有高度的可重复性(参见"Efficient disruption of animal tissues"、"Reproducible RNA purification from plant tissues"、"Reproducible DNA purification from animal tissue"、"Purification of intact protein from animal tissue"、"Reproducible RNA purification from Gram-positive bacteria"和"Reliable detection of miRNAs")。
Principle

基因分型、基因表达分析和蛋白质组学研究均要求对生物样本进行高效破碎,以确保获得高产的DNA、RNA和蛋白质。TissueLyser II体系通过研磨珠的高速震荡来实现对样本的有效破碎。TissueLyser II可快速、彻底的破碎样本,彻底释放生物分子,并可同时对样本进行匀,协助后续使用的QIAGEN试剂盒进行纯化(参见"QIAGEN purification kits compatible with QIAGEN disruption systems")。

QIAGEN提供从样本收集到DNA、RNA和蛋白质纯化的完整解决方案,TissueLyser II是其中关键的组成部分。TissueLyser II可作为QIAsymphony SP等QIAGEN自动化仪器高通量制备和分析样本的补充(参见"QIAGEN high-throughput automation")。这一即用型仪器可从1–96个样本中自动纯化DNA、RNA和蛋白质。TissueLyser II也适用于QIAGEN手动样本制备试剂盒(参见"QIAGEN purification kits compatible with QIAGEN disruption systems")。

进行RNA研究时,新鲜收集的组织可在RNAlater RNA Stabilization Reagent中稳定,可有效避免操作过程中RNA的降解。对于需要纯化DNA、 RNA和蛋白质的应用,可使用Allprotect Tissue Reagent,将新鲜收集的组织中的待分析物迅速稳定。

<>QIAGEN高通量自动化仪器
仪器 目的 通量
QIAsymphony SP核酸提取纯化分析仪 纯化DNA、RNA和蛋白质   每批1–96个样本
BioRobot Universal System 纯化DNA和RNA 96孔板
QIAxcel 对DNA片段和RNA进行电泳分析 每批多达96个样本
QIAgility 反应体系构建 每批多达384个样本
Rotor-Gene Q实时荧光定量PCR分析仪 real-time PCR和HRM分析  每批多达100个样本
PyroMark Q96 MD or ID 甲基化和突变分析 每批多达96个样本
与QIAGEN破碎仪器兼容的QIAGEN纯化试剂盒
纯化的被分析物 样本类型 QIAGEN试剂盒
RNA 容易裂解的组织  RNeasy Kits
RNeasy Plus Kits
RNeasy Protect Kits
RNA 富含纤维的组织 RNeasy Fibrous Tissue Kits
RNA 各种类型组织 RNeasy Lipid Tissue Kits
RNeasy Universal Tissue Kits
RNA 植物组织 RNeasy Plant Mini Kit
RNA 酵母 RNeasy Kits
RNA 细菌 RNeasy Protect Bacteria Kits
microRNA 各种类型组织 miRNeasy Kits
DNA 人类组织 QIAamp DNA Kits
DNA 动物组织 DNeasy Blood & Tissue Kits
蛋白质 组织 Qproteome Mammalian Protein Prep Kit
磷蛋白 组织 PhosphoProtein Purification Kit
糖蛋白 组织 Qproteome Glycoprotein Kits
DNA和RNA 组织 AllPrep DNA/RNA Kits
DNA、RNA和蛋白质 组织 AllPrep DNA/RNA/Protein Mini Kit
Procedure

TissueLyser Adapter Sets使用微型收集管,可处理多达2 x 96个样本,或使用2 ml微型离心管,处理多达2 x 24个样本。如果在样本破碎过程中不使用裂解缓冲液,则需要将适配器置于–80°C环境中预冷。在破碎时样品管需保持密封,以避免交叉污染,这对于real-time RT-PCR或芯片分析等高敏感性的下游应用尤其重要。根据样本类型不同,破碎使用不锈钢珠、碳化钨珠或玻璃珠进行。TissueLyser Bead Dispensers用来方便的将单个珠子加入到离心管中,或同时将96个研磨珠加入到收集管中。TissueLyser II也可配合Grinding Jar Sets使用,破碎大体积样本。

Applications

TissueLyser II每批可处理多达192个样本,因此是遗传学、转录组学和蛋白质组学研究中获得生物信息的理想解决方案。TissueLyser II是新一代高通量测序的首选破碎仪器。TissueLyser II可与多种QIAGEN样本纯化试剂盒配套使用,样本纯化可以手动进行,也可以在QIAcube全自动核酸纯化仪、QIAsymphony SP核酸提取纯化分析仪、EZ1 Advanced、BioRobot或BioSprint工作站上自动进行。

Feature
Specifications
Disruption principle High-speed shaking of samples in 1.2 ml collection tubes or 2 ml microcentrifuge tubes with stainless steel or glass beads
Features Convenient and secure disruption process. Adapter sets optimized for high-throughput disruption. Wide range of accessories available (e.g.,grinding jar set to process large samples). Reproducible results with difficult-to-lyse tissues. Front-end solution for QIAGEN automation.
Kits compatible with instrument All kits for purifying RNA/DNA/Protein
Protocol/main application on this intrument Sample preparation/sample disruption
Technical data 100–120/220–240 V, 50/60Hz; variable speeds from 3 to 30 Hz (180–1800 oscillations/minute)
Technology Bead Mill
Throughput 2 x 96 collection microtubes (1.2 ml) or 2 x 24 microcentrifuge tubes (2ml)
Typical run time 15 sec - 2 x 3 minutes at 15–30 Hz

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Kit Handbooks
1
For high-throughput disruption of biological samples
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Supplementary Protocols
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