预混合的PCR反应液方便PCR体系构建
  • QIAGEN PCR缓冲体系最大限度地减少了PCR条件的优化
  • 预混液规格易于反应体系构建
  • 更少的移液操作最大限度减少污染风险
Taq PCR Master Mix包含Taq DNA Polymerase、独特的QIAGEN PCR Buffer最大限度的减少优化需求,以及dNTP。方便的2倍预混液减少了移液步骤,提高了反应的通量和可重复性,同时降低了污染的风险。
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Taq PCR Master Mix Kit (250 U)
3 x 1.7 ml Taq PCR Master Mix containing 250 units Taq DNA Polymerase, 3 x 1.7 ml Distilled water
201443
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Taq PCR Master Mix Kit (1000 U)
12 x 1.7 ml Taq PCR Master Mix containing 1000 units Taq DNA Polymerase, 12 x 1.7 ml Distilled water
201445
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The Taq PCR Master Mix Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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Reproducible PCR.|Tolerance to variable magnesium concentration.|Tolerance of different primer Tm values.|Lot-to-lot reproducibility.|Specific amplification of long PCR products.|Increased specificity of primer annealing.|
A fragment of the hepatitis B surface antigen gene (gene S) was amplified from 10, 20, and 50 copies of target template, using the Taq PCR Master Mix Kit. Five parallel amplifications were performed for each amount of starting template DNA. Equal volumes of the PCR products were analyzed on a 2% agarose gel. C: negative control; M: markers.|PCR amplification was performed with the QIAGEN PCR Buffer and Taq DNA Polymerase (QIAGEN), using the indicated Mg2+ concentrations. The same PCR was performed in parallel using a PCR buffer and Taq  polymerase from another supplier (Supplier AII). The single-copy human prion protein gene was amplified successfully in each case using the buffer and enzyme from QIAGEN. M: markers.|The human single-copy cystic fibrosis gene was amplified with Taq DNA Polymerase and the QIAGEN PCR Buffer using the indicated annealing temperatures. Primers employed were a 22mer with a Tm of 57.5°C (GC content: 54.5%) and a 32mer with a Tm of 85.2°C (GC content: 78%).  For analysis, 10% of a 100 µl reaction was loaded on an agarose gel. M: markers.|A fragment of the single-copy gene for cystic fibrosis was amplified from 30 ng, 3 ng, and 300 pg human genomic DNA corresponding to 104, 103, and 102 copies of target template, respectively. Three different lots of QIAGEN's Taq DNA Polymerase were used and equal volumes of the PCR product were analyzed on a 1% agarose gel. M: markers.|Three different-sized products from human genomic DNA were amplified using eitherTaq DNA Polymerase and PCR Buffer (QIAGEN), or a polymerase and buffer from another supplier (Supplier AII). For analysis, 10% of each reaction was loaded on the gel. Results from duplicate PCR amplifications are shown. M: markers.|Ammonium and potassium cations in QIAGEN PCR Buffers increase specificity of primer annealing. K+ binds to the phosphate groups (P) on the DNA backbone, stabilizing the annealing of the primers to the template. NH4+, which exists both as the ammonium ion and as ammonia under thermal-cycling conditions, can interact with the hydrogen bonds between the bases (B), destabilizing the weak hydrogen bonds at mismatched bases. The combined effect of the two cations maintains the high ratio of specific-to-nonspecific primer-template binding over a wide temperature range.|
Performance
Taq PCR Master Mix Kit相比其他供应商测试的试剂盒有更卓越的表现,并在大量的PCR应用中有可靠的PCR性能,无需耗时的优化过程。该试剂盒包含Taq DNA Polymerase,一种高品质的重组酶适用于常规的和特殊的PCR应用(参见"Reproducible PCR")。Taq DNA Polymerase确保在不同的引物模板体系中高特异性扩增(参见"Tolerance of different primer Tm values"和"Specific amplification of long PCR products")。每批QIAGEN's Taq DNA Polymerase受到全面地质量监控测试,包括严格的PCR特异性和重复性测试,在此测试中可从人类基因组DNA中扩增低拷贝靶(参见"Lot-to-lot reproducibility")。由于预混液中还包含独特的PCR缓冲液,可通过改变退火温度或Mg2+浓度,即可显著降低优化过程,而且通常不需要优化(参见"Wide annealing temperature window"和"Tolerance to variable magnesium concentration")。
Taq DNA Polymerase规格

浓度:5单位/µl
重组酶:
底物类似物:dNTP、ddNTP、dUTP、biotin-11-dUTP、DIG-11-dUTP、荧光-dNTP/ddNTP
延伸率:72°C下2–4 kb/分钟
半衰期:97°C下10分钟;94°C下60分钟
扩增效率:≥105
5'–>3'外切酶活性:
Extra A辅助剂:
3'–>5'外切酶活性:
污染核酸:
污染RNA酶:
污染蛋白酶:
自吸泵活性:

Principle

Taq PCR Master Mix Kit包含预混合规格的QIAGEN's Taq DNA Polymerase。这种易于使用的反应液同样包含QIAGEN PCR Buffer、MgCl2和具优化浓度的超纯dNTP。只需加入引物和模板DNA即可构建PCR反应体系。由于方便的预混液规格,最大限度地减少了移液失误,确保了高重复的PCR结果(参见"Reproducible PCR")。Taq PCR Master Mix可在2–8°C下储存长达2个月,通过去除解冻时间来构建更快速的PCR反应体系。

Taq DNA Polymerase

Taq DNA Polymerase是高品质的重组酶,适用于常规的和特殊的PCR应用(参见"Tolerance of different primer Tm values"和"Specific amplification of long PCR products")。

QIAGEN PCR Buffer 

研发新型的QIAGEN PCR Buffer通过减少使用时对PCR条件的优化,来节省时间和精力。QIAGEN PCR Buffer包括KCl和(NH4)2SO4。独特的缓冲体系有助于特异性PCR产物的扩增。在每个PCR循环的退火步骤中,该缓冲液具有特异性-非特异性引物的高比率结合。由于KCl和(NH4)2SO4独特的平衡结合,相比传统的PCR缓冲液,该PCR缓冲液提供更大范围退火温度和Mg2+浓度条件,以及严格的引物退火条件。通过改变退火温度或Mg2+浓度,即可显著减少PCR优化过程,而且通常不需要优化(参见"Wide annealing temperature window"和"Tolerance to variable magnesium concentration")。

Procedure
Taq PCR Master Mix包含Taq DNA Polymerase、QIAGEN PCR Buffer、MgCl2和优化浓度的dNTP。即用型2倍预混液规格最大限度减少了移液食物,同时提供了极大便利。构建PCR反应体系快速、简单、直接,只需加入引物和模板DNA。该试剂盒还易于操作的实验方案,确保了在第一次操作中就可获得特异性扩增并成功进行PCR反应。
Applications

Taq PCR Master Mix Kit适用于常规的和特殊的应用,包括:

  • 常规的PCR
  • RT-PCR
  • 筛选 
  • 基于PCR的DNA指纹图谱分析(VNTR、STR和RAPD)
Feature
Specifications
Applications PCR, RT-PCR, DNA fingerprinting
dNTP's included Yes (in Master Mix)
Enzyme activity 5' -> 3' exonuclease activity
Mastermix Yes
Reaction type PCR amplification
Real-time or endpoint Endpoint
Sample/target type Genomic DNA and cDNA
Single or multiplex Single
With/without hotstart Without hotstart

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Kit Handbooks
1
For standard and specialized PCR applications with minimal optimization
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References
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