用于常规和特殊的PCR
  • QIAGEN PCR缓冲体系最大限度地减少了PCR条件的优化
  • 独特的即用型PCR缓冲液,操作更快速简单
  • Q-Solution可帮助扩增GC含量高的模板
  • 提供多种规格的包装方便使用
QIAGEN PCR Buffer中的Taq DNA Polymerase最大限度的减少了使用时对PCR条件的优化,新型的辅助剂Q-Solution则有助于实现“困难”模板(比如,GC含量高的模板)的高效扩增。CoralLoad PCR Buffer(包含两种胶示踪染料)使PCR产物可以直接上样电泳。
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Taq DNA Polymerase (250 U)
250 units Taq DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 5x Q-Solution, 25 mM MgCl2
201203
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Taq DNA Polymerase (1000 U)
4 x 250 units Taq DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 5x Q-Solution, 25 mM MgCl2
201205
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Taq DNA Polymerase (5000 U)
20 x 250 units Taq DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 5x Q-Solution, 25 mM MgCl2
201207
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Taq DNA Polymerase Kit (25000 U)
100 x 250 units Taq DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 5x Q-Solution, 25 mM MgCl2
201209
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The Taq DNA Polymerase is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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CoralLoad PCR Buffer.|Tolerance to variable magnesium concentration.|Amplification of difficult templates.|Tolerance of different primer Tm values.|Lot-to-lot reproducibility.|Specific amplification of long PCR products.|Increased specificity of primer annealing.|
[A]  CoralLoad Concentrate contains two gel-tracking dyes, enabling immediate gel loading of PCR products for [B ] easy visualization of DNA migration.|PCR amplification at the indicated Mg2+ concentrations using QIAGEN PCR Buffer and Taq DNA Polymerase (QIAGEN). The same PCR was performed in parallel using a PCR buffer and Taq polymerase from another supplier (Supplier AII). The single-copy human prion protein gene was amplified successfully in each case using the buffer and enzyme from QIAGEN. M: markers.|Two different primer-template systems were amplified in duplicate using QIAGEN PCR Buffer and Taq DNA Polymerase in the absence () or presence (+) of 1x Q-Solution. Q-Solution enables specific amplification of difficult templates. [A] human angiotensin receptor II gene; [B]  mouse protein kinase C gene;  M: markers.|The human single-copy cystic fibrosis gene was amplified with Taq DNA Polymerase and QIAGEN PCR Buffer using the indicated annealing temperatures. Primers employed were a 22mer with a Tm of 57.5°C (GC content: 54.5%) and a 32mer with a Tm of 85.2°C (GC content: 78%). For analysis, 10% of a 100 µl reaction was loaded. M: markers.|A fragment of the single-copy gene for cystic fibrosis was amplified from 30 ng, 3 ng, and 300 pg human genomic DNA corresponding to 104, 103, and 102 copies of target template, respectively. Three different lots of Taq DNA Polymerase were used and equal volumes of the PCR product were analyzed on a 1% agarose gel. M: markers.|Three different-sized products from human genomic DNA were amplified using either Taq DNA Polymerase and QIAGEN PCR Buffer (QIAGEN), or Taq polymerase and a buffer from another supplier (Supplier AII).  For analysis, 10% of each reaction was loaded on the gel. Results from duplicate PCR amplifications are shown. M: markers.|Ammonium and potassium cations in QIAGEN PCR Buffers increase specificity of primer annealing. K+ binds to the phosphate groups (P) on the DNA backbone, stabilizing the annealing of the primers to the template. NH4+, which exists both as the ammonium ion and as ammonia under thermal-cycling conditions, can interact with the hydrogen bonds between the bases (B), destabilizing the weak hydrogen bonds at mismatched bases. The combined effect of the two cations maintains the high ratio of specific-to-nonspecific primer-template binding over a wide temperature range.|
Performance

Taq DNA Polymerase可在多种PCR条件下进行灵敏的PCR反应,无需耗时的优化过程(参见"Tolerance of different primer Tm Values" 和"Specific amplification of long PCR products")。每批Taq DNA Polymerase都受到全面的质量控制检测,包括严格的PCR特异性和可重复性分析,从人类基因组DNA扩增低拷贝的目的基因(参见"Lot-to-lot reproducibility")。试剂盒提供的QIAGEN PCR Buffer和CoralLoad PCR Buffer具有独特的组成,可在多种PCR条件下进行高度特异性的PCR,无需优化(参见"Wide annealing-temperature window" 和"Tolerance to variable magnesium concentration")。此外,CoralLoad PCR Buffer使得PCR产物可直接上样到琼脂糖凝胶,更易于操作,更快获得结果。试剂盒中提供的Q-Solution可进一步提高PCR的性能(参见"Amplification of difficult templates")。

Taq DNA Polymerase的规格

浓度: 5 单位/µl 
重组酶: 是
底物类似物: dNTP、ddNTP、dUTP、biotin-11-dUTP、DIG-11-dUTP和fluorescent-dNTP/ddNTP
延伸速率: 72°C 2–4 kb/min 
半衰期: 97°C 10 min;94°C 60 min
扩增效率: ≥105
5'–>3'外切酶活性: 有
额外添加A: 有
3'–>5'外切酶活性: 无
核酶污染: 无
RNases污染: 无
蛋白酶污染: 无
自引发活性: 无

Principle

Taq DNA Polymerase是一种高品质重组酶,适合常规和专门的PCR应用(参见"Tolerance of different primer Tm Values" 和"Specific amplification of long PCR products")。

QIAGEN PCR Buffer

研发的新型QIAGEN PCR Buffer可节省时间和精力,减少所需PCR优化。QIAGEN PCR Buffer含有KCl和(NH4)2SO4 。独特的缓冲液便于扩增特异性PCR产物。在每个PCR循环的退火步骤,缓冲液使引物结合具有高特异性比率。KCl和(NH4)2SO4独特比例结合,使PCR缓冲液与常规PCR缓冲液相比,可在更宽范围的退火温度和Mg2+浓度下提供严格的引物退火条件。极大减少通过改变退火温度或Mg2+浓度的PCR优化过程,有时甚至不需要(参见"Wide annealing temperature window" 和"Tolerance to variable magnesium concentration")。

CoralLoad PCR Buffer

CoralLoad PCR Buffer具有QIAGEN PCR Buffer的所有优点。此外,还可直接将PCR反应液上样到琼脂糖凝胶,无需再单独添加凝胶上样缓冲液。与常规QIAGEN PCR Buffer一样,CoralLoad PCR Buffer具有相同的PCR特异性和最少的反应优化。另外,缓冲液还含有两种标记染料:一种橙色染料和一种红色染料,便于估计DNA迁移距离和优化琼脂糖凝胶电泳时间(参见"CoralLoad PCR Buffer")。缓冲液提高了移液可视性,使PCR产物可直接上样到凝胶,提高了便利性。

Q-Solution

Q-Solution通过修饰DNA的熔解行为,便于扩增GC含量高的模板或含有高度二级结构的模板。使用这种独特的试剂常常能完成或改进不理想的PCR(参见"Amplification of difficult templates")。与DMSO和其他PCR添加剂不同,Q-Solution可在多种引物-模板体系中使用特定工作浓度,而不会产生毒性作用。

Procedure
Taq DNA Polymerase可在多种应用中进行高度特异性的PCR,减少PCR参数的优化过程。该试剂盒简化的、易于操作的流程使PCR反应体系构建更加简单。CoralLoad PCR Buffer也增加便利性和易操作性。PCR产物可直接上样到凝胶中,无需添加上样染料。可提升PCR性能的Q-Solution确保成功处理GC含量高的模板。
Applications

Taq DNA Polymerase适用于常规和特殊的应用,包括:

  • 常规PCR
  • RT-PCR
  • 筛选 
  • 基于PCR的DNA指纹图谱分析(VNTR、STR和RAPD)
Feature
Specifications
Applications PCR, RT-PCR, DNA fingerprinting
dNTP's included No
Enzyme activity 5' -> 3' exonuclease activity
Mastermix No
Reaction type PCR amplification
Real-time or endpoint Endpoint
Sample/target type Genomic DNA and cDNA
Single or multiplex Single
With/without hotstart Without hotstart

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Kit Handbooks
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For standard and specialized PCR applications with minimal optimization
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