配合Rotor-Gene Q实时荧光定量PCR分析仪使用

  • 加快反应体系构建,提高通量
  • 配合Rotor-Gene Q实时荧光定量PCR分析仪进行real-time PCR,表现卓越

Rotor-Disc 100 Starter Kit提供在Rotor-Gene Q实时荧光定量PCR分析仪上进行100个反应所需的所有试剂和耗材。

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Product Cat. no. List price:
Rotor-Disc 100 Starter Kit (230V)
3 Rotor-Disc 100 packs for 15–25 µl reaction volumes, Rotor-Disc Heat Sealer, Rotor-Disc Heat Sealing Film, Rotor-Disc 100 Rotor and Locking Ring, Rotor-Disc Pipetting Aid, Rotor-Disc Easy Lift Adaptor
9001603
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The Rotor-Disc 100 Starter Kit is for use with the Rotor-Gene Q thermal cycler.


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Principle

Rotor-Disc 100 Starter Kit为Rotor-Disc 100孔盘规格,可在Rotor-Gene Q实时荧光定量PCR分析仪上便利的进行real-time PCR。该试剂盒含有Rotor-Disc 100孔盘、Rotor-Disc Heat Sealer、Rotor-Disc Heat Sealing Film、Rotor-Disc 100 Rotor和Locking Ring、Rotor-Disc 100 Loading Block、以及Rotor-Disc Pipetting Aid。

Rotor-Discs与QIAgility仪器兼容,因此反应体系构建可自动进行。

Procedure

Rotor-Disc 100 Rotor和Rotor-Disc 100 Locking Ring与Rotor-Disc 100孔盘配套使用。Rotor-Disc 100孔盘为圆形,有100个垂直的反应孔,适合高通量应用。Rotor-Disc 100孔盘相当于圆形的96孔板,外加4个对照孔。因此,平时使用96孔板进行实验的实验室可以便利的用Rotor-Gene Q实时荧光定量PCR分析仪进行操作。多出的孔可放置更多样本、对照,不会占据96孔。为无缝整合到96孔板工作流程中,Rotor-Disc 100孔盘使用96孔板的标记方法,如:从A1-A12到H1-H12。多出的4个孔被标记为R1-R4。

Rotor-Disc 100孔盘无需管盖。只需将Rotor-Disc Heat Sealing Film置于孔的顶部,用Rotor-Disc Heat Sealer加热即可密封。牢固、结实的密封膜可避免污染。

Applications

使用Rotor-Gene Q实时荧光定量PCR分析仪和Rotor-Disc 100 Starter Kit可进行real-time PCR和高分辨率熔解分析,包括:

  • 基因表达分析
  • 病原体检测
  • DNA甲基化分析
  • 基因分型
  • 基因扫描
  • miRNA研究

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Brochures & Guides
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Protocol Files
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Calibration guide for use with QIAgility and the Rotor-Disc 100
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