从各种类型组织中纯化总RNA,配有gDNA Eliminator Solution
  • 适用于所有组织溶液类型
  • 整合RNeasy和QIAzol技术,节省时间
  • 不使用酶,快速去除基因组DNA
  • RNA纯度高,适用于下游应用

RNeasy Plus Universal Kits可快速、方便的纯化RNA,同时有效地去除基因组DNA。优化的实验方案可从各种组织类型,包括难裂解组织中纯化高品质RNA。该试剂盒含有QIAzol Lysis Reagent和RNeasy离心柱,前者用于裂解脂肪组织和其他组织类型,后者用于纯化高品质RNA。该试剂盒有两种规格:RNeasy Plus Universal Mini Kit可从至多50 mg组织中纯化RNA,RNeasy Plus Universal Midi Kit可从至多250 mg组织中纯化RNA。RNeasy Plus Universal Mini Kit可在QIAcube全自动核酸纯化仪上自动化进行纯化。

Product Product no. Cat. no. List price:
 
 
Show details
    varies
Can't order online?
To place an order via phone, email or for requesting a quote, please provide the product’s name, number and catalog number.
Product Cat. no. List price:
RNeasy Plus Universal Mini Kit (50)
For 50 RNA minipreps: RNeasy Mini Spin Columns, gDNA Eliminator Solution, Collection Tubes, RNase-Free Water and Buffers
73404
Inquire
RNeasy Plus Universal Midi Kit (10)
For 10 RNA midipreps: RNeasy Midi Spin Columns, gDNA Eliminator Solution, Collection Tubes, RNase-Free Water and Buffers
73442
Inquire

RNeasy Plus Universal Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.


  • Main Image Navi
Effective removal of genomic DNA.|High-quality RNA.|RNeasy Plus Universal Mini and Midi procedures.|
RNA was isolated from the indicated amounts of RNAlater stabilized rat kidney using the RNeasy Plus Universal Mini Kit, or was precipitated using a precipitation reagent from Supplier AII. RNA eluates were analyzed by real-time RT-PCR, with and without reverse transcription, using the QuantiFast Probe RT-PCR Kit and the Rotor-Gene Q.|RNA was purified from rat tissue using the RNeasy Plus Universal Mini Kit, with or without gDNA Eliminator Solution. [A] Real-time RT-PCR analysis was carried out using RNA isolated from 25 mg frozen liver tissue. The CT value of all samples was comparable. DNA contamination was detected in the –RT control if gDNA Eliminator Solution was not used, whereas the samples isolated using gDNA Eliminator Solution showed minor or no DNA contamination. [B] RNA was purified from 25 mg stabilized heart tissues. Data obtained using the QIAxcel system demonstrates good RNA integrity regardless of whether RNA was isolated using gDNA Eliminator Solution.||
Performance

RNeasy Plus Universal操作流程可从各种类型的组织中得到高品质RNA,重复性好,适用组织包括脂肪组织和不易裂解的组织(参见下表)。不同物种和不同发育阶段的组织的纯化产量不同,尤其是脂肪组织。

RNeasy Plus Universal Kits纯化得到总RNA的产量
大鼠/小鼠组织(10 mg) 总RNA产量 (µg)
脂肪组织  0.5–2.5
5–20
心脏 5–25
10–60
5–40
15–80
5–15
肌肉 5–35
皮肤 2–5
15–100

使用real-time RT-PCR和QIAxcel体系分析纯化得到的RNA,表明这些RNA具有较好的完整性(参见"High-quality RNA")。gDNA Eliminator Solution和RNeasy技术可高效去除大部分基因组DNA污染,无需进行DNase处理(参见"Effective removal of genomic DNA")。

Principle

RNeasy Plus Universal操作流程可从各种类型组织中纯化RNA,包括脂肪或不易裂解的组织。RNeasy Plus Universal Kits整合了苯酚/胍裂解技术和硅胶膜总RNA纯化技术。试剂盒中的QIAzol Lysis Reagent是一种由苯酚和硫氰酸胍组成的单相溶液,专为方便的裂解各种组织设计,并能抑制RNases的活性。高效的裂解以及通过有机抽提去除污染物,使RNeasy离心柱可用于处理更大量的组织。gDNA Eliminator溶液能有效去除基因组DNA污染,非常有助于DNA分离到中间相。

Procedure

RNeasy Plus Universal操作流程易于进行,与苯酚/氯仿处理后回收相比,此方法更快、更有效,能得到更高纯度和产量的RNA(参见"RNeasy Plus Universal Mini and Midi procedures")。RNeasy Plus Universal Mini Kit可从50 mg组织中纯化RNA;RNeasy Plus Universal Midi Kit可从250 mg组织中纯化RNA。

可使用TissueRuptor、TissueLyser LT或TissueLyser II在QIAzol Lysis Reagent中进行样本匀浆。加入gDNA Eliminator Solution和氯仿后,匀浆离心分为水相和有机相。收集含有RNA的上层水相,加入乙醇,使用RNeasy离心柱纯化RNA。总RNA结合到离心柱膜上,苯酚和其他污染物被高效洗去。获得的高品质RNA可用RNase-free水洗脱下来。

该实验方案适用于富集mRNA,不包括大部分小于200 nt的RNAs(如rRNAs和tRNAs)。RNeasy Plus Universal操作流程稍作修改后可从培养细胞中纯化包含miRNA等小RNAs的总RNA。

Applications

RNeasy Plus Universal Kits纯化得到的RNA非常适用于对少量DNA敏感的下游应用,如定量real-time RT-PCR。纯化得到的RNA还可以用于其他应用。

Feature
Specifications
Applications Northern, dot, and slot blotting, end-point RT-PCR, quantitative, real-time RT-PCR, array analysis, next-generation sequencing
Elution volume Mini: 30µl; Midi:150µl
For automated processing Mini format can be automated on the QIAcube
Format Mini and Midi Format
Integrated removal of genomic DNA Yes
Main sample type Tissue samples including difficult-to-lyse and demanding tissues
Processing Manual (centrifugation), automated (QIAcube)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein RNA
Sample amount Mini: 5-50 mg; Midi: 50-250 mg
Technology QIAzol/Spin column technology

You are not authorized to download the resource

FAQs
22
FAQ ID -1024
View
FAQ ID -2338
View
FAQ ID -2339
View
FAQ ID -2340
View
FAQ ID -2341
View
FAQ ID -2342
View
FAQ ID -2343
View
FAQ ID -2344
View
FAQ ID -2345
View
FAQ ID -2346
View
FAQ ID -2656
View
FAQ ID -2657
View
FAQ ID -2793
View
FAQ ID -2794
View
FAQ ID -2796
View
FAQ ID -2797
View
FAQ ID -2798
View
FAQ ID -2799
View
FAQ ID - 3390
View
FAQ ID -627
View
FAQ ID -728
View
FAQ ID - 3388
View
Kit Handbooks
1
For purification of total RNA from all types of tissue
Show details