用于在QIAcube全自动核酸纯化仪上自动纯化细胞和组织中的总RNA

  • 预装载的转子适配器,可快速构建体系
  • 高效的工作流程,可得到标准化结果
  • 有助于避免人工上样失误
  • 试剂盒包含自动纯化所需的所有试剂
  • 每批可自动纯化12个样本

RNeasy Mini QIAcube Kit是RNeasy Mini Kit产品中适用于在QIAcube全自动核酸纯化仪上使用的试剂盒,使用方便。RNeasy Mini QIAcube Kit可从细胞和组织中纯化高品质RNA,产量稳定。

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RNeasy Mini QIAcube Kit (240)
For 240 RNA minipreps: RNeasy Mini Rotor Adapters (preloaded with spin columns and elution tubes); RNase-free reagents and buffers
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The RNeasy Mini QIAcube Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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Significant time savings.|
The y-axis shows a comparison of the average percentage of hands-on time required for sample prep using manual processing, using the QIAcube, and using the QIAcube with dedicated kits.|
Performance
RNeasy Mini QIAcube Kit含转子适配器,预装了RNeasy离心柱和洗脱管,十分方便省时(参见"Significant time savings with dedicated QIAcube Kits")。
Principle
RNeasy Mini QIAcube Kit含转子适配器,预装了RNeasy离心柱和洗脱管,增加操作的便利性,减少人工失误。该试剂盒所含试剂专为在QIAcube全自动核酸纯化仪上自动纯化RNA而设计,减少浪费。试剂盒内不含手动操作所需的试管。RNeasy Mini QIAcube Kit使用严谨的异硫氰酸胍裂解法和高纯度的硅胶膜纯化方法,可从多达1 x 107个细胞或30 mg组织中标准化纯化RNA。
Procedure

QIAcube全自动核酸纯化仪使用先进的技术处理QIAGEN离心柱,可将自动化低通量样本制备流程无缝整合到实验室工作流程中。所有纯化步骤都可实现完全自动化,每批可处理多达12个样本。QIAcube全自动核酸纯化仪与RNeasy Mini QIAcube Kit配合使用,可快速、简单、方便的纯化RNA。

Applications

RNeasy Mini QIAcube Kit纯化得到的高品质RNA适用于多种下游应用,如:

定量real-time RT-PCR
芯片分析
Feature
Specifications
Applications PCR, qPCR, real-time RT-PCR, microarray
Elution volume 30–100 µl
Format Spin column, preloaded into rotor adapters
Main sample type Tissue, cells
Processing Automated (QIAcube)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Total RNA
Sample amount 0.5–30 mg
Technology Silica technology
Yield Varies

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