收集样本、稳定RNA、运输和保存组织

  • 立即稳定和保护RNA
  • 可靠的基因表达和分析数据
  • 温室下操作方便安全
  • 可长期保存组织,无RNA降解风险
  • 方便收集并保存于可重复密封的管中
RNAlater TissueProtect Tubes为螺旋口管,包括RNAlater RNA Stabilization Reagent,用于立即稳定组织样本中的RNA以保存基因表达谱。可在室温下稳定进行,无需使用干冰或液氮。
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RNAlater TissueProtect Tubes (50 x 1.5 ml)
For stabilization of RNA in 50 x 150 mg tissue samples: 50 screw-top tubes containing 1.5 ml RNAlater RNA Stabilization Reagent each
76154
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RNAlater TissueProtect Tubes (20 x 5 ml)
For stabilization of RNA in 20 x 500 mg tissue samples: 20 screw-top tubes containing 5 ml RNAlater RNA Stabilization Reagent each
76163
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RNAlater TissueProtect Tubes are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.


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RNAlater TissueProtect Tubes prevent RNA changes in tissues.|RNAlater Reagent prevents degradation of mRNA in tissues.|Stable RNA in tissues: different temperatures and freeze–thaw cycles.|
Rat tissues (10 mg each) were excised and stored at room temperature for up to 4 hours in phosphate-buffered saline (PBS) or RNAlater RNA Stabilization Reagent (RNAlater). At the indicated times, RNA was isolated using the RNeasy Protect Mini Kit. Total RNA was used for quantitative RT-PCR using the QuantiTect Probe RT-PCR Kit and primers and probe specific for the transforming growth factor β (TGF-β) gene or the tumor necrosis factor α (TNF-α) gene. Analyses were carried out in triplicate on the ABI PRISM 7700 Sequence Detection System. The change in threshold cycle (CT) is shown, relative to the CT at time zero.|RNA was isolated from fresh rat kidney samples after 0, 5, 10, 15, 30, and 60 minutes, using either standard procedures (Unstabilized) or RNeasy Protect Kits (Stabilized). The RNA isolated was analyzed by agarose gel electrophoresis and expression of GAPDH was examined using northern blot analysis. M: markers.|Total RNA was isolated from tissue samples stored in RNAlater RNA Stabilization Reagent. [A] Rat lung tissue was stored under the indicated conditions. RNA was analyzed on a formaldehyde agarose gel and northern blot (GAPDH-probed). [B] Stabilized mouse liver tissue was subjected to freeze–thaw cycles (-20°C/25°C). C: control tissues frozen in liquid nitrogen and stored at -80°C.|
Performance
RNAlater Stabilization Reagent可在收集和储存样本时稳定和保护RNA表达谱(参见"RNAlater Reagent prevents degradation of mRNA in tissues")。RNAlater技术对RNA的立即保护可确保下游分析真实地反映整个组织样本的表达谱(参见"RNAlater TissueProtect Tubes prevent RNA changes in tissues")。样本可长期保存,无RNA降解风险,甚至经多次冻融后也无影响。在从多种条件下保存的RNAlater稳定样本中纯化得到的RNA,适用于northern印迹和RT-PCR等下游应用(参见"Stable RNA in tissues: different temperatures and freeze–thaw cycles")。
Principle

一旦生物样本被收集后,其RNA会变得极不稳定。创新的液状RNAlater RNA Stabilization Reagent可快速渗入组织,立即稳定RNA,保护RNA表达模式。还能避免样本处理导致的基因诱导或下调,可进行准确的基因表达分析。

Procedure

组织样本收集后,迅速浸入适当体积的RNAlater RNA Stabilization Reagent中,即可保护RNA,保存样本。用可重复密封的管子,RNAlater TissueProtect Tubes可预先测定所需RNAlater RNA Stabilization Reagent的体积,方便操作和样本保存。该试剂可使RNA在37°C下保存1天,18–25°C下保存7天,2–8°C下保存4周,并且可以在无干冰或液氮条件下处理、储存和运输样本。另外,样本也可以在–20°C或–80°C的条件下存档。

Applications

使用RNAlater RNA Stabilization Reagent稳定的动物和人类组织可用QIAGEN仪器进行样本破碎,并与QIAGEN试剂盒配合使用,进行:

总RNA纯化
miRNA纯化
mRNA纯化

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Kit Handbooks
1
RNAlater TissueProtect Tubes - For collection of harvested animal tissues with immediate stabilization of the gene expression profile, and subsequent transport and storage; RNAlater RNA Stabilization Reagent - For immediate stabilization of the gene expression profile in harvested animal tissues
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