超快速纯化至多15 μg测序级别纯的质粒DNA

  • 快速的一步裂解
  • 减少手动操作时间和实验步骤
  • 1.5–3 ml菌液至多可获得15 μg DNA
  • 9分钟可获得即用型质粒DNA

QuickLyse Miniprep Kit为提取测序级别的质粒DNA提供了一个以快速离心柱为基础的方法。在单一的缓冲液中,细菌细胞重悬、溶解,同时与DNA结合,使得在较短的操作时间内快速纯化DNA。

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QuickLyse Miniprep Kit (100)
100 QuickLyse Spin Columns, Reagents, Buffers, Lysis and Collection Tubes (2 ml)
27405
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QuickLyse Miniprep Kit (250)
250 QuickLyse Spin Columns, Reagents, Buffers, Lysis and Collection Tubes (2 ml)
27406
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The QuickLyse Miniprep Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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High yields of high-quality plasmid DNA.|QuickLyse procedure.|Reproducible yields.|Long read-lengths in automated sequencing.|
Typical DNA yield and purity from 1.5 ml of bacterial culture (LB media) for different cells and vectors.
|The simple bind-wash-elute procedure of the QuickLyse system.
|Plasmid DNA (pSV-β-galactosidase) was purified using the QuickLyse Miniprep Kit (QL), as well as three additional commercially available kits (13). Purified plasmid DNA was digested with NdeI and EcoRI restriction enzymes. The reactions were performed in duplicate. M: markers. Ø: uncut plasmid.|Typical sequencing results for plasmid DNA purified using the QuickLyse Miniprep Kit. The pSV-β-galactosidase plasmid was isolated from TOP10 cells and sequenced using the BigDye terminator sequencing chemistry.
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Performance

QIAGEN QuickLyse Miniprep Kit采用一步裂解技术,可以超速制备至多15 µg质粒DNA。DNA的质量确保了其在下游应用中的良好表现,如测序、限制性分析和克隆(参见"Reproducible yields")。

QuickLyse Miniprep Kit纯化的足量质粒DNA,可以用于任何的下游应用,包括测序(参见 "Long read-lengths in automated sequencing")。从1.5 ml的细菌培养液中至多获得15 µg的高拷贝质粒DNA(参见 "High yields of high-quality plasmid DNA")。纯化得到的质粒DNA有1.7–1.9的OD260/280 比率,表明其质量和纯度适合多种敏感应用。而且很容易获得超过700个单位的读取长度。

Principle

不同于大多数方案需要3步裂解程序,QuickLyse Miniprep Kit将细菌细胞裂解的酶和渗透压处理步骤融合到一个3分钟步骤中。此外,QuickLyse技术使用很少量的缓冲溶液、简化操作并且节约大量时间:可以在22分钟内获得24份小量纯化的质粒DNA。

Procedure
重悬浮和裂解的细菌细胞和DNA结合在单一的缓冲溶液中,使得DNA在短时间内得到快速纯化。在3分钟的裂解步骤后,澄清的裂解产物直接加入到QuickLyse Spin Column中。质粒DNA结合在离心柱膜上,然后用简单的清洗步骤洗脱。(参见"QuickLyse procedure")。得到的DNA可以直接运用在标准的应用中,包括自动测序、PCR、限制性分析和克隆。
Applications

QuickLyse Miniprep Kit产生足量的质粒DNA用于多种的下游应用,包括自动测序。

Feature
Specifications
Applications Automated sequencing, PCR, restriction analysis, cloning etc.
Culture volume/starting material 1.5 ml culture volume
Elution volume 50 µl
Plasmid type High-copy, cosmid DNA
Processing Manual (centrifugation)
Samples per run; throughput 1–24 samples per run
Technology QuickLyse technology
Time per run or prep per run 9 min
Yield 3–7 µg

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Kit Handbooks
1
For purification of sequencing grade plasmid DNA
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