真空驱动处理多至24个离心柱

  • 减少样品操作,加快处理
  • 平行样品处理

QIAvac 24 Plus真空歧管用于同时真空处理多个QIAGEN离心柱。

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QIAvac 24 Plus
Vacuum manifold for processing 1–24 spin columns: includes QIAvac 24 Plus Vacuum Manifold, Luer Plugs, Quick Couplings
19413
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Vacuum-driven liquid processing on vacuum manifolds.|High, reproducible yields of genomic DNA.|An integrated system for various applications.|
|Genomic DNA was purified from 200 μl whole EDTA-treated blood stored at 4°C for 3 days. Eluate (20 μl) was loaded per lane. M: markers.|The QIAvac 24 Plus, Connecting System, and Vacuum Pump allow collection of infectious material.|
Principle
真空驱动的液体处理方法可加速样本操作,无需在离心机中安装并拆除离心柱的繁琐过程,也无需在结合和洗涤过程中去除流出液,因此减少了手工制备时间(参见"Vacuum-driven liquid processing on vacuum manifolds")。在从敏感样本中纯化核酸时,防止交叉污染尤其重要。将QIAGEN离心柱插入一次性的VacConnectors中,防止直接与离心柱接触。使用VacValves可确保黏稠性样本(如血液)接触的真空压力稳定。QIAvac 24 Plus和QIAvac Connecting System是方便处理多种QIAGEN试剂盒的理想组合。
Procedure

将1–24个离心柱插入歧管中,加入样本或缓冲液,打开真空泵。样本和缓冲液在压力下通过离心柱,便于大体积样本上样,不必弃去流出液。真空驱动的液体处理方法比离心方法的速度快,并且产量高、可重复(参见"High, reproducible yields of genomic DNA")。

配合QIAvac Connecting System,QIAvac 24 Plus可作为流通系统(参见"An integrated system for various applications")。可能含有有害物质的流出液被收集在单独的废物瓶里。

Applications

使用QIAvac 24 Plus真空底座可快速、高效的真空处理QIAGEN离心柱,可用于实验室研究。真空驱动的液体处理方法可代替离心操作,减少样本制备的手工操作时间。

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Kit Handbooks
1
For vacuum processing of QIAGEN spin columns  
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User-Developed Protocols
2
This procedure has been adapted by customers and is for purification of viral RNA and DNA from plasma, serum, and cell-free body fluids using the QIAamp MinElute Virus Vacuum Kit.
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The procedure has been used successfully for isolation of a single-copy, 14.5 kb, binary plasmid, p35S GUS INT, from Agrobacterium tumefaciens strain GV2260 (1).
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Safety Data Sheets
1