整合了完整工作流程的自动化体系,从样本制备到反应体系构建
  • 创新、易于使用的模块化系统,带有内置型触摸屏
  • 从多种类型样本中纯化DNA、RNA和蛋白
  • 自动将洗脱液转移到QIAsymphony AS模块进行反应体系构建
  • 连续上样,通过条形码扫描实现样本追踪
  • 输入输出样本列表
使用专用QIAsymphony Kit,QIAsymphony SP核酸提取纯化分析仪可从多种起始样本中提取DNA、RNA、以及细菌和病毒核酸。QIAsymphony AS拓展了QIAsymphony SP核酸提取纯化分析仪的功能,使其与PCR反应体系自动化构建接合,再配合Rotor-Gene Q实时荧光定量PCR分析仪和QIAGEN real-time和终点法PCR试剂盒,使PCR工作流程自动化,极大提高工作效率。
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QIAsymphony SP
QIAsymphony sample prep module: includes 1-year warranty on parts and labor
9001297
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QIAsymphony SP System
QIAsymphony sample prep module: includes installation and training, 1-year warranty on parts and labor
9001751
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QIAsymphony SP Priority Package
QIAsymphony sample prep module: includes Priority Package with software, installation, training, 2-year warranty on parts and labor, and 2 preventive maintenance visits
9001880
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QIAsymphony SP Priority Package Plus
QIAsymphony sample prep module: includes Priority Package with software, installation, training, 3-year warranty on parts and labor, and 3 preventive maintenance visits
9001881
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QIAsymphony AS
QIAsymphony assay setup module: includes 1-year warranty on parts and labor
9001301
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QIAsymphony Cabinet SP/AS
Accessory for correct positioning of the QIAsymphony SP/AS instruments
9020246
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QIAsymphony Cabinet AS
Accessory for correct positioning of the QIAsymphony AS instrument
9020245
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QIAsymphony Cabinet SP
Accessory for correct positioning of the QIAsymphony SP instrument
9020244
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QIAsymphony SP核酸提取纯化分析仪应与QIAsymphony Kits配合使用, 适用于试剂盒使用手册中指定的应用。QIAsymphony AS应与可与其兼容的QIAGEN Kits配合使用,适用于试剂盒使用手册中指定的应用。如果将QIAsymphony AS与非QIAGEN的试剂盒配合使用,用户应自行确认是否适用于相关应用。


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QIAsymphony workflows.|Prefilled, sealed reagent cartridges.|QIAsymphony Cabinet SP/AS.|
The QIAsymphony SP/AS instruments can be operated in independent or integrated mode.|Reagent cartridges are prefilled with all reagents required for the purification procedure, including accessory enzymes, and are labeled with 2D bar codes. Partially used cartridges can be resealed for later use, giving you complete cost control.|The QIAsymphony Cabinet SP and the QIAsymphony Cabinet AS are designed for correct positioning of the QIAsymphony SP/AS instruments.|
Principle

QIAsymphony SP核酸提取纯化分析仪能处理1–96个样本,每批24个,样本体积至多2 ml。一系列专门的QIAsymphony Kits能提高实验室效率,只需几个试剂盒配合优化的实验方案就能囊括广泛的起始材料和应用。QIAsymphony技术整合了硅胶膜纯化的速度和效率,以及磁珠纯化的便利性。

适用于一系列起始材料的专门的QIAsymphony Kits
QIAsymphony Kit 起始材料和用量
QIAsymphony DNA Kit 可从至多1000 µl人类全血、400 µl白膜层、50 mg组织(包括FFPE组织)或1 x 107个细胞中纯化DNA,可从血液中纯化病毒核酸
QIAsymphony DNA Investigator Kit 为人类身份鉴定和法医学纯化参考和个案样本
QIAsymphony DSP Virus/Pathogen Kit 从血浆、血清和脑脊液中纯化病毒核酸,从呼吸道和泌尿生殖系统的样本中纯化病毒或细菌核酸
QIAsymphony DSP AXpH DNA Kit 运用AXpH技术从液体细胞学样本中纯化DNA
QIAsymphony RNA Kit 从50 mg组织(包括FFPE组织)或1 x 107个细胞中纯化总RNA和miRNA
QIAsymphony PAXgene Blood RNA Kit* 从在PAXgene Blood RNA Tubes中收集的2.5 ml人类全血中纯化细胞内RNA(包括miRNA)
QIAsymphony mericon Bacteria Kit 从500 μl富集培养物中纯化细菌核酸
QIAsymphony DSP DNA Kit 从至多1000 µl人类全血、400 µl白膜层、组织(包括FFPE组织)、细菌和细胞培养液中纯化DNA,从血液中纯化病毒核酸
QIAsymphony Certal Kits 从纯化缓冲液、细胞培养基上清样本和疫苗制备样本中纯化残留的宿主细胞DNA和病毒核酸
* 每次可处理多至72个样本。

将QIAsymphony SP核酸提取纯化分析仪拓展到QIAsymphony AS,实现了整个流程的自动化。此系统可消除手动移液步骤产生的人为误差,非常适合于每日需要进行复杂的PCR分析的实验室。QIAsymphony AS可保持试剂、洗脱液和分析试剂的活性,确保安全、可重复性的分析体系构建。 QIAsymphony AS能够冷却试剂、洗脱液等,能够安全的进行反应体系构建。QIAsymphony AS每轮可构建多个反应体系,支持QIAGEN PCR和RT-PCR试剂盒。可提供上门检测分析的定制服务。

QIAsymphony SP/AS仪器设计新颖,操作过程具有杰出的安全性和标准化。吸头监控将交叉污染降至最低,内置的UV灯可对工作台面高效灭菌。对样本和试剂的条形码读取可实现纯化和分析体系构建的全程样本追踪。 可提供按时间排序的电子文档结果,包括样本ID信息、位置以及样本制备和PCR试剂的货号和有效期。多水平的用户管理可记录处理样本的是何用户。电子结果文件可通过联网的打印机自动打印,也可使用USB棒下载后存档。

QIAsymphony Cabinet SP/AS(参见"QIAsymphony Cabinet SP/AS")为正确放置QIAsymphony SP/AS仪器专门设计。QIAsymphony Cabinet SP/AS由两部分组成,分别用于放置QIAsymphony SP核酸提取纯化分析仪和QIAsymphony AS。如果有QIAsymphony SP核酸提取纯化分析仪和QIAsymphony AS仪器,需要柜子的两部分并列放置。如果只有QIAsymphony SP核酸提取纯化分析仪,只需要QIAsymphony Cabinet SP。QIAsymphony Cabinet SP和QIAsymphony Cabinet AS都有废物隔层,用于盛装工作台弹出的用过的吸头。

Procedure

即用型QIAsymphony SP核酸提取纯化分析仪只需最简单的设置。已装有QIAsymphony SP核酸提取纯化分析仪的实验室可再添加QIAsymphony AS模块,简单升级系统。QIAsymphony AS可与QIAsymphony SP核酸提取纯化分析仪直接接口。为了减少手工操作步骤,降低样本污染风险,在QIAsymphony SP核酸提取纯化分析仪上处理的样本可自动转移到QIAsymphony AS(完整操作模式)。为增加灵活性,QIAsymphony SP核酸提取纯化分析仪和AS也可独立操作。之前处理的样本或在不同QIAsymphony SP核酸提取纯化分析仪上处理的样本都能手动转移到QIAsymphony AS(参见"QIAsymphony workflows")。

样品制备和分析体系构建可通过触摸屏定义。简单地将需要的样本、试剂和消耗品上样到样品仓。当样品仓关闭,会进行详细的库存扫描。只能保证工作台为选择的实验方案进行正确设置。样品制备完成后,可从洗脱仓中取出纯化的核酸或蛋白,也可通过转移模块将洗脱液直接转移到QIAsymphony AS进行分析体系构建。分析体系构建后,可直接将体系转移到real-time PCR仪进行分析。

样品输入仓可兼容一系列样品形式,以适应各种工作流程和样品制备需求。可支持条形码标记的一级和二级样品管(直径10–16.9 mm)、微孔板和块板。每批可连续上样24个样本和内参,每批可选取不同实验方案,提高效率和自动化时间。QIAsymphony AS可对每次运行或每个样本设置一个或多个PCR,分离洗脱液进行检测,或对洗脱液、试剂和PCRs进行冷却。选择不同适配器可使用不同real-time PCR仪(如Rotor-Gene Q实时荧光定量PCR分析仪、96-well cyclers或32-capillary cyclers)。使用Rotor-Gene Q实时荧光定量PCR分析仪进行检测,只需在Strip Tubes或Rotor-Discs中简单设置PCR。

QIAsymphony Kits具有杰出的安全性和易用性。即用型、条形码标记的试剂筒预装有纯化流程所需的所有试剂,包括配套的酶(参见"Prefilled, sealed reagent cartridges")。快速的工作台设置可为您节约宝贵的时间。只需将两个试剂筒装入QIAsymphony仓。可使用相同或不同试剂盒的试剂筒,用于对96个样本进行不同纯化操作流程。试剂筒可被仪器自动打开,使用的试剂量适合样本数量,为您提供完整的成本控制。

Applications

QIAsymphony SP/AS适用于多种实验室应用,包括:

  • 生物医学研究
  • 人类身份鉴定
  • 兽医应用
  • 生物安全研究
  • 生命科学应用,如基因组学和蛋白质组学
Software

QIAsymphony操作软件设置了一个样本制备或分析体系构建的程序,可对使用者每一步进行指导,并可选择和采用标准化实验方案。缓冲液、试剂、预混液和对照所需体积都能自动计算,并告知使用者需要添加消耗品的数量和种类。从QIAsymphony SP向QIAsymphony AS传输样本上样信息十分方便,减少定义分析体系所需的步骤。

QIAsymphony SP/AS仪器提供标准化的连接接口。工作列表和机架文件能被输入到此仪器,在实验室收到样本之前就能设定自动化程序。分析体系构建后,样本列表可输出到real-time PCR仪。这种双向数据交换能将QIAsymphony SP/AS仪器整合到实验室信息管理系统(LIMS)中。

Feature
Specifications
Altitude Up to 2000 m
Elution volume QIAsymphony SP — 30–500 µl (application dependent)
Humidity Relative humidity of 15–75% (noncondensing); maximum 75% relative humidity for temperatures up to 31°C, decreasing linearly to 50% humidity at 32°C
Input volume QIAsymphony SP — minimum input volume: 200 µl (application dependent)
Instrument dimensions QIAsymphony SP, 128 x 103 x 73 cm; QIAsymphony AS, 59 x 103 x 73 cm; QIAsymphony SP/AS (integrated operation), 185 x 103 x 73 cm
Kits or Application Packs designed for this instrument QIAsymphony SP — QIAsymphony DSP Virus/Pathogen Kits and other QIAsymphony Kits; QIAsymphony AS — artus QS-RGQ Kits (not available in all countries), Rotor-Gene Kits and other QIAGEN real-time and end-point PCR kits
Operating temperature 15–32°C
Overvoltage category II
Place of operation For indoor use only
Pollution level 2
Power 100–240 V AC, 50–60 Hz, 1400 VA, mains supply voltage are not to exceed 10% of nominal supply voltages
Processing Automated
Protocols/main application on this instrument QIAsymphony SP — Purification of DNA, RNA, and bacterial and viral nucleic acids from a wide range of starting materials; QIAsymphony AS — PCR setup.
Storage temperature 5–40°C in manufacturer's package
Technology QIAsymphony SP — QIAGEN magnetic-particles chemistry
Transportation temperature –25 to 70°C in manufacturer's package
Weight QIAsymphony SP, 175 kg; QIAsymphony AS, 90 kg; QIAsymphony SP/AS (integrated operation), 265 kg

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Instrument Technical Documents
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QIAsymphony Cabinet SP/AS documents
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QIAsymphony Cabinet SP/AS document
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Script change document for software version 4.0
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Script change document for software version 4.0
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Script change document for software version 4.0
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Script change document for software version 4.0
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Script change document for software version 4.0
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QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0
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QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0
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QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0
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QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0
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Differences between QIAsymphony software versions
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Operating Software
3
Labware package SOW-516-6 for use with the QIAsymphony SP/AS instruments (software version 4.0)
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QIAsymphony Management Console operating software for QIAsymphony software version 4.0.3
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Differences between QIAsymphony software versions
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Protocol Files
5
For use with the QIAsymphony SP/AS instruments (software version 4.0)
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Instrument User Manuals
5
For use with QIAsymphony SP/AS instruments (software versions 4.0 and 4.1)
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Application/Protocol Documents
27
For use with the QIAsymphony AS (software version 4.0)
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