从组织或细胞中自动纯化总RNA,或是含有miRNA的总RNA

  • 预装试剂条,使用方便
  • 标准化的操作确保高重复性
  • 经优化的操作得到高产量、高纯度的RNA
  • 条形码实现样本和试剂的追踪

QIAsymphony RNA Kit可在QIAsymphony SP核酸提取纯化分析仪上从1–96个样本中自动纯化总RNA,或是含有miRNA的总RNA和其他小分子RNA。可从动物细胞、组织或者福尔马林固定、石蜡包埋组织中纯化总RNA,需要的起始材料量少(≥100个细胞),至多1 x 107个细胞或50 mg的组织。经优化的实验方案可获得高产量、高纯度的总RNA,或是含有miRNA的总RNA;洗脱体积灵活,因而可以根据不同下游应用优化调整RNA浓度。预装试剂条减少了工作站设置时间,并减少了手动操作。条形码阅读器进行试剂全程跟踪。

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QIAsymphony RNA Kit
For as many as 192 preps, depending on protocol: includes 2 reagent cartridges and enzyme racks and accessories
931636
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The QIAsymphony RNA Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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Reproducible purification of RNA.|Automated purification comparable to manual procedure.|Highly pure intact RNA.|Efficient handling of magnetic particles.|Prefilled, sealed reagent cartridges.|
RNA was purified from Jurkat cells on the QIAsymphony SP using the QIAsymphony RNA Kit. RNA yields from 2 instrument runs using 2 different reagent cartridge lots were compared with manual purification using the RNeasy Mini Kit. Each bar represents the average of 4 samples.|[A] RNA was purified from rat kidney and spleen samples (10 mg each sample) stabilized in RNAlater RNA Stabilization Reagent on the QIAsymphony SP using the QIAsymphony RNA Kit. [B] RNA was purified from rat kidney and spleen samples (10 mg each sample) stabilized in RNAlater RNA Stabilization Reagent manually using the RNeasy Mini Kit. RNA yield with the QIAsymphony RNA Kit is compared to manual isolation using the RNeasy Mini Kit by agarose gel electrophoresis.|RNA purified from Jurkat cells on the QIAsymphony SP had RIN (RNA integrity number) values between 9.9 and 10, and 28S/18S rRNA ratios of 2.0–2.1, indicating that intact RNA was isolated.|The QIAsymphony SP processes a sample containing magnetic particles as follows: A magnetic rod protected by a rod cover enters a well containing the sample and attracts the magnetic particles. The magnetic rod with cover is positioned above another well and releases the magnetic particles. The QIAsymphony SP uses a magnetic head containing an array of 24 magnetic rods, and can therefore process 24 samples simultaneously. Steps 1 and 2 are repeated several times during sample processing.|Reagent cartridges are prefilled with all reagents required for the purification procedure, including accessory enzymes, and are labeled with 2D bar codes. Partially used cartridges can be resealed for later use giving you complete cost control.|
Performance
在QIAsymphony SP工作站上的RNA自动纯化流程与手动纯化流程相同,从培养细胞、组织和福尔马林固定、石蜡包埋组织中,纯化得到高产量、高品质RNA(参见"Reproducible purification of RNA")。QIAsymphony纯化总RNA的实验方案主要纯化大于200个核苷酸的RNA,包括mRNA(参见"Highly pure, intact RNA")。QIAsymphony miRNA实验方案也纯化总RNA,但不排除<200个核苷酸的RNA,如 miRNA和其他小分子RNA(参见"Automated purification comparable to manual procedure")。
Principle
QIAsymphony RNA Kit将硅胶膜技术的速度、效率与易于操作的磁性颗粒相结合(参见"Efficient handling of magnetic particles")。在蛋白酶K处理后,纯化>200个核苷酸的总RNA,或包含miRNA的总RNA和其他小分子。起始材料包括动物细胞和组织。配合一些额外的组分,可以用该试剂盒纯化福尔马林固定、石蜡包埋组织。 裂解样本,然后通过将其结合到离液盐中的硅胶表面上的颗粒中,将RNA从裂解物中一步纯化。使用磁珠将颗粒从裂解物分离,然后使用不含RNase的DNase处理,去除DNA。充分洗涤磁珠,随后用不含RNase的纯水洗脱得到RNA。
Procedure

创新的即用型试剂条预装有磁性颗粒和所有纯化过程需要的试剂,包括附加的酶。这些试剂条可在QIAsymphony SP工作站上被自动打开,有助于降低污染的风险(参见"Prefilled, sealed reagent cartridges")。台面设置快速,节省你的宝贵时间。只需简单的在试剂置入模块中加载至多2个试剂条。试剂条可以来自相同的试剂盒,也可以是不同的试剂盒,因此可在处理96个样本的同一批次中,运行不同的纯化步骤。试剂仅会用于所选数目的样本,实现成本控制。纯化操作流程包括4 个步骤:裂解、结合、洗涤和洗脱。提供多种在QIAsymphony SP工作站上进行RNA纯化的实验方案,取决于起始材料和所需的RNA产物(参见下表)。

使用QIAsymphony SP纯化RNA的实验方案
实验方案 配套使用 样本材料(预处理样本裂解物) 处理体积(μl)
RNA CT 400 QIAsymphony RNA Kit  (192) ≤3 x 106个培养细胞
≤20 mg易裂解的组织
400 μl
RNA CT 800 QIAsymphony RNA Kit (2 x 48) 3 x 106–1 x 107个培养细胞
≤50 mg易裂解的组织
800 μl
RNA FT 400* QIAsymphony RNA Kit (192) ≤20 mg纤维组织 400 μl
miRNA CT 400* QIAsymphony RNA Kit (192) ≤3 x 106个培养细胞
≤20 mg组织
400 μl
RNA FFPE 130 QIAsymphony RNA Kit (2 x 72) 1–2 x FFPE切片至多10 μm
1 x FFPE切片20 μm
130 μl
* 需要使用蛋白酶K,需要单独订购。
需要其他组分,需要单独订购。
Applications

使用QIAsymphony RNA Kit在QIAsymphony SP核酸提取纯化分析仪上实现总RNA的全自动纯化过程,得到高品质RNA,广泛用于各种下游应用。

Feature
Specifications
Applications RT-PCR; Real-Time PCR; Expression Profiling
Elution volume 50-200 µl
Sample amount 400 µl lysate from <20 mg tissue or 3 x 10e6 cells or 800 µl lysate from <50 mg tissue or 3 x 10e6 cells

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