用于整合的样本制备、反应体系构建,手动转移样本至检测模块,满足分子检测所需
  • 超高灵活性
  • 优化的操作流程,高实用性
  • 十分便利,易于操作
  • 兼容实验室开发测试
  • 适合多种应用
这一全面的工作流程解决方案中包含用于样本制备的QIAsymphony SP、和用于反应体系构建的QIAsymphony AS,以及在Rotor-Gene Q实时荧光定量PCR分析仪上进行的一系列即用型分析。QIAGEN将进一步研发、拓展QIAsymphony系列产品。
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QIAsymphony Cabinet SP/AS
Accessory for correct positioning of the QIAsymphony SP/AS instruments
9020246
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QIAsymphony SP核酸提取纯化分析仪应与可与其兼容的QIAsymphony Kits配合使用,适用于试剂盒使用手册中指定的应用。QIAsymphony AS应与可与其兼容的QIAGEN Kits配合使用,适用于试剂盒使用手册中指定的应用。如果将QIAsymphony AS 与非QIAGEN的试剂盒配合使用,用户应自行确认是否适用于相关应用。Rotor-Gene Q实时荧光定量PCR分析仪应与可与其兼容的QIAGEN Kits配合使用,适用于试剂盒使用手册中指定的应用。如果将Rotor-Gene Q实时荧光定量PCR分析仪与非QIAGEN的试剂盒配合使用,用户应自行确认是否适用于相关应用。


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QIAsymphony workflows.|Highly sensitive detection of CMV DNA.|Highly sensitive detection of EBV DNA.|Highly sensitive detection of HBV DNA.|Highly sensitive detection of HCV RNA.|Highly sensitive detection of HIV-1 RNA|
There are 2 modes of system operation: integrated or independent.|Probit analysis: CMV (QIAsymphony RGQ system). Analytical sensitivity of the artus CMV QS-RGQ Kit with the QIAsymphony RGQ system using EDTA plasma.|Probit analysis: EBV (QIAsymphony RGQ system). Analytical sensitivity of the artus EBV QS-RGQ Kit with the QIAsymphony RGQ system using EDTA plasma.|Probit analysis: HBV (QIAsymphony RGQ system). Analytical sensitivity of the artus HBV QS-RGQ Kit with the QIAsymphony RGQ system.|Probit analysis: HCV (QIAsymphony RGQ system). Analytical sensitivity of the artus HCV QS-RGQ Kit with the QIAsymphony RGQ system.|Probit analysis: HI Virus-1 (QIAsymphony RGQ system). Analytical sensitivity of the artus HI Virus-1 QS-RGQ Kit with the QIAsymphony RGQ system.|
Performance

QIAsymphony RGQ配合artus QS-RGQ Kits,应用于多种高灵敏度、高特异性检测:

CMV DNA(参见"Highly sensitive detection of CMV DNA")
EBV DNA(参见"Highly sensitive detection of EBV DNA")
HBV DNA(参见"Highly sensitive detection of HBV DNA")
HIV-1 RNA(参见"Highly sensitive detection of HIV-1 RNA")
HCV RNA(参见"Highly sensitive detection of HCV RNA")
Principle

QIAsymphony RGQ可处理多达96个样本,从样本到结果只需一次完整的自动化运行,节省时间。一系列专门的试剂盒可组成分子检测的开放或封闭系统。

QIAsymphony RGQ是完整的体系,能够提高效率,优化样本到结果的工作流程。其包含QIAsymphony AS/SP的自动样本制备和反应体系构建,以及Rotor-Gene Q实时荧光定量PCR分析仪的检测功能。QIAsymphony SP核酸提取纯化分析仪可自动完成多种类型样本的制备。QIAsymphony AS确保构建的PCR反应体系高度可靠且重复性好,适合每日需要进行复杂PCR分析的实验室。QIAsymphony AS可对每次运行或样本进行多重体系构建,支持artus QS-RGQ的产品,CE-IVD标准的工作流程,也支持实验室自己设定的工作流程。Rotor-Gene Q实时荧光定量PCR分析仪可配合CE-IVD-marked的artus QS-RGQ试剂盒或自己设定的工作流程进行敏感、精确的real-time PCR分析。

在QIAsymphony RGQ上完全兼容CE-IVD标准的试剂盒
试剂盒 仪器 方法
QIAsymphony DSP Virus/Pathogen Kits QIAsymphony SP核酸提取纯化分析仪 从多种类型的样本中纯化病毒核酸或细菌DNA
artus QS-RGQ Kits QIAsymphony AS Real-time PCR(多重;样本和内参)
在QIAsymphony RGQ上适合自己设定工作流程测试的试剂盒
应用 试剂盒 仪器 方法
纯化 QIAsymphony DSP Virus/Pathogen Kits QIAsymphony SP核酸提取纯化分析仪 从各种类型样本中纯化病毒核酸或细菌
QIAsymphony DSP AXpH DNA Kit QIAsymphony SP核酸提取纯化分析仪 从细胞培养悬浮液中纯化病毒核酸或细菌
QIAsymphony Certal Kits QIAsymphony SP核酸提取纯化分析仪 从生物纯化缓冲液、细胞培养悬浮液样本和疫苗制备样本中纯化宿主残留DNA和病毒核酸
反应体系构建
—病原体检测
QuantiFast Pathogen +IC Kits QIAsymphony AS 使用序列特异性探针进行多重real-time PCR和RT-PCR
QuantiTect Virus Kits QIAsymphony AS 可在单管中高度灵敏的检测多种病毒DNA和RNA
QIAGEN OneStep RT-PCR Kit QIAsymphony AS 使用任何RNA模板,简单设置,进行灵敏的RT-PCR
反应体系构建
—基因表达
Rotor-Gene SYBR® Green Kits QIAsymphony AS 使用SYBR® Green I进行超快速、精确的real-time PCR和RT-PCR
Rotor-Gene Probe Kits QIAsymphony AS 使用序列特异性的探针进行超快速、精确的real-time PCR和RT-PCR
Rotor-Gene Multiplex Kits QIAsymphony AS 使用序列特异性的探针进行超快速、精确的多重real-time PCR和RT-PCR
反应体系构建
—残留DNA 检测
Certal Detection Kits QIAsymphony AS 多重real-time PCR检测残留DNA和内参
Procedure

QIAsymphony RGQ工作流程的开始是在QIAsymphony SP仪器上纯化核酸。从样本制备流程中得到的含纯核酸的洗脱液转移到QIAsymphony AS模块,进行分析体系构建。分析样本再手动转移到Rotor-Gene Q实时荧光定量PCR分析仪进行PCR和数据分析。

为了满足不同工作流程需要,操作系统中有两种模式:完整模式或独立模式。在完整模式中,样本在QIAsymphony SP核酸提取纯化分析仪处理后直接转移到QIAsymphony AS,减少了手工操作步骤和记录。为了增加灵活性,QIAsymphony SP核酸提取纯化分析仪和AS也可在独立模式中进行相互独立的操作。参见"QIAsymphony workflows"。

Applications

QIAsymphony RGQ是目前和将来用于各种分子检测的最具有多功能性的系统。QIAsymphony RGQ在一个平台上整合了多种分析,也能进行自行开发测试。

适合于开放和闭合系统多种应用,包括:

  • artus移植系列检测产品(CMV、EBV、HSV、BK病毒和VZV)
  • artus血源性病毒面板(HIV、HCV和HBV)
  • 实验室自行开发的测试
Feature
Specifications
Altitude Up to 2000 m (6500 ft.)
Elution volume QIAsymphony SP — 30–500 µl (application dependent)
Features Rotor-Gene Q — dynamic range, 10 orders of magnitude
Heat dissipation/thermal load Rotor-Gene Q — average, 0.183 kW (632 BTU/hour); peak, 0.458 kW (1578 BTU/hour)
Humidity QIAsymphony SP/AS — relative humidity of 15–75% (noncondensing); maximum 75% relative humidity for temperatures up to 31°C, decreasing linearly to 50% humidity at 32°C
Rotor-Gene Q — relative humidity of 10–75% (noncondensing)
Input volume QIAsymphony SP — minimum input volume: 200 µl (application dependent)
Instrument dimensions QIAsymphony SP/AS — QIAsymphony SP, 128 x 103 x 73 cm; QIAsymphony AS, 59 x 103 x 73 cm; QIAsymphony SP/AS (integrated operation), 185 x 103 x 73 cm
Rotor-Gene Q — width, 37 cm (14.6 in.); height, 28.6 cm (11.3 in.); depth (without cables), 42 cm (16.5 in.); depth (door open), 53.8 cm (21.2 in.)
Kits or Application Packs designed for this instrument QIAsymphony SP — QIAsymphony DSP Virus/Pathogen Kits and other QIAsymphony Kits; QIAsymphony AS — artus QS-RGQ Kits (not available in all countries), Rotor-Gene Kits and other QIAGEN real-time and end-point PCR kits
Rotor-Gene Q — artus QS-RGQ Kits (not available in all countries), RG SYBR Green PCR Kits; RG SYBR Green RT-PCR Kit; RG Probe PCR Kits; RG Probe RT-PCR Kit; RG Multiplex PCR Kit
Operating temperature QIAsymphony SP/AS — 15–32°C (59–89.6ºF)
Rotor-Gene Q — 18–30ºC (64–86ºF)
Optical System Rotor-Gene Q — up to 6 channels spanning UV to infra-red wavelengths; excitation sources: high energy light-emitting diodes; detector: photomultiplier; acquisition time: 4 s; the software allows to create new excitation/detection wavelength combinations
Overvoltage category II
Place of operation For indoor use only
Pollution level QIAsymphony SP/AS — 2
Rotor-Gene Q — 2; Environmental class 3K2 (IEC 60721-3-3)
Power QIAsymphony SP/AS — 100–240 V AC, 50–60 Hz, 1400 VA, mains supply voltage are not to exceed 10% of nominal supply voltages
Rotor-Gene Q — 100–240 V AC, 50–60 Hz, 520 VA (peak); Power consumption 8 VA (standby); Mains supply voltage fluctuations are not to exceed 10% of the nominal supply voltages; F5A 250 V fuse
Processing QIAsymphony SP/AS — automated
Protocols/main application on this instrument QIAsymphony SP — purification of DNA, RNA, and bacterial and viral nucleic acids from a wide range of starting materials
QIAsymphony AS — PCR setup
Rotor-Gene Q — gene expression analysis, microRNA detection, virus detection, SNP genotyping, SNP genotyping, High Resolution Melt analysis (HRM)
Samples per run; throughput QIAsymphony SP — 1–96 samples in batches of 24
Rotor-Gene Q — tubes 0.2 ml; strip tubes 0.1 ml (4 tubes); Rotor-Disc 72; Rotor-Disc 100; up to 100 samples per run using a Rotor-Disc 100
Software Rotor-Gene Q — Rotor-Gene Q software, supplied on the provided installation CD
Storage temperature QIAsymphony SP/AS — 5–40°C (41ºF to 104ºF) in manufacturer's package
Rotor-Gene Q — 15 to 30ºC (59ºF to 86ºF) in manufacturer’s package; max. 75% relative humidity (noncondensing); environmental class 1K2 (IEC 60721-3-1)
Technology QIAsymphony SP — QIAGEN magnetic-particles chemistry
Rotor-Gene Q — real-time PCR cycler
Thermal performance Rotor-Gene Q — temperature range, 35 to 99ºC; temperature accuracy, ±0.5ºC (type, measured 30 seconds after clock start); temperature resolution, ±0.02ºC (smallest programmable increment); temperature uniformity, ±0.02ºC; ramp rate (peak ramp rates, air), >15ºC/s heating, >20ºC/s cooling
Transportation conditions QIAsymphony SP/AS — –25 to 70°C (–13ºF to 158ºF) in manufacturer's package
Rotor-Gene Q — –25 to 60ºC (–13ºF to 140ºF) in manufacturer’s package; max. 75% relative humidity (noncondensing); environmental class 2K2 (IEC 60721-3-2)
Typical run time Rotor-Gene Q — 40 cycles in 45 min with the QIAGEN RG Kits (assay dependent)
Warranty Rotor-Gene Q — 1 year on instrument; lifetime guarantee on excitation LEDs
Weight QIAsymphony SP, 175 kg; QIAsymphony AS, 90 kg; QIAsymphony SP/AS (integrated operation), 265 kg; Rotor-Gene Q, 12.5 kg (27.6 lb.) (standard configuration)

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Instrument Technical Documents
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Labware Documents
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For use with the QIAsymphony SP/AS instruments (software version 4.0)
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