QIAsymphony PAXgene Blood RNA Kit

从稳定的血液中自动纯化胞内RNA(包括miRNA)

  • 提高RNA品质和产量
  • 有效的miRNA共纯化
  • 操作安全、易于使用的创新预装试剂条
  • 标准化的处理流程实现高度重复性
  • 条形码扫描追踪样本和试剂

QIAsymphony PAXgene Blood RNA Kit提供全自动的胞内RNA 纯化,适用于QIAsymphony SP核酸提取纯化分析仪。创新的改进试剂盒确保了更高品质的产物RNA,包括miRNA。与PAXgene Blood RNA Tubes(由BD和BD授权代理商提供,货号762165)配合使用进行样本采集和稳定,此系统采用经验证的磁珠技术,提供一个完整的分析前处理方案,用于样本采集和稳定及纯化高品质的RNA。

Product Product no. Cat. no. List price:
 
 
Show details
    varies
Can't order online?
To place an order via phone, email or for requesting a quote, please provide the product’s name, number and catalog number.
Product Cat. no. List price:
QIAsymphony PAXgene Blood RNA Kit (96)
For 96 preps: 2 Reagent Cartridges, Enzyme Racks, accessories, and RNase-free buffers
762635
Inquire
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
  • Main Image Navi
High-quality RNA from different donors.|On-board measures against cross-contamination.|QIAsymphony PAXgene Blood RNA procedure.|
Blood was collected from 12 donors into PAXgene Blood RNA Tubes. RNA was purified using the QIAsymphony PAXgene Blood RNA Kit on the QIAsymphony SP. RNA was analyzed with an Agilent 2100 Bioanalyzer. Clear enrichment of small RNA species is shown in the gel-like image by the corresponding peaks in the donor RNA next to the marker band.|Tip guards minimize the risk of sample-to-sample cross-contamination. [A] Pipetting channels. [B] Extractor unit.|[A] QIAsymphony SP instrument. [B] Schematic of the QIAsymphony SP principle.|
Performance

全自动的PAXgene Blood RNA实验方案可获得与手动PAXgene Blood RNA实验方案相当的 RNA产量,通常2.5 ml健康人类全血(4.6 x 106 – 1.1 x 107白细胞/ml)的RNA产量为≥3 μg,其中>95%的样品经PAXgene Blood RNA Kits处理。自动化的PAXgene Blood RNA实验方案可从不同供体样本中纯化高品质小分子RNA(参见"High-quality RNA from different donors")。由于产量与供体高度相关,个体产量可能不同。DNase处理可降低基因组DNA污染。纯化得到的RNA可以即用于下游应用,如real-time RT-PCR。

Principle

QIAsymphony PAXgene Blood RNA Kit提供全自动的胞内RNA 纯化,适用于QIAsymphony SP工作站。与PAXgene Blood RNA Tubes(由BD和BD授权代理商提供,货号 762165)配合使用进行样本采集和稳定,此系统采用经验证的磁珠技术,提供一个完整的分析前处理方案,用于样本采集和稳定及纯化高品质的 RNA。预装试剂条将手工操作减少到最小,通过条形码可实现试剂全程追踪。

Procedure
将血液样品(2.5 ml)收集在PAXgene Blood RNA Tubes中,可在室温下储存或运输。纯化过程的第一步是通过离心使每个PAXgene Blood RNA Tube中的样品沉淀,沉淀重悬后,将试管转移到QIAsymphony SP工作台上。载入的试剂盒和耗材由专用软件指导和检查。之后QIAsymphony SP工作站就可开始全自动的RNA纯化过程。DNase处理确保将基因组DNA污染最小化(参见"QIAsymphony PAXgene Blood RNA procedure")。灵敏和标准化的操作流程配合QIAsymphony SP工作站内部处理,可将样品与样品之间的交叉污染最小化(参见"On-board measures against cross-contamination")。纯化之后,通过最后加热处理,提高洗脱液在下游应用中的表现。处理24个样品的整个流程大约2.5小时,其中手工操作时间最多为25–30分钟。
Applications

使用QIAsymphony PAXgene Blood RNA Kit和QIAsymphony SP核酸提取纯化分析仪可纯化得到高纯度RNA,可广泛地用于各种敏感的下游应用,包括:

RT-PCR和定量real-time RT-PCR
表达谱芯片分析 
cDNA合成
RNase和S1核酸酶保护分析
Northern印迹、点印迹和狭缝印迹 
Feature
Specifications
Applications Quantitative, real-time RT-PCR, expression array and chip analysis, next-generation sequencing, cDNA synthesis, RNase and S1 nuclease protection, northern, dot, and slot blot analysis, primer extension
Elution volume 80 µl or 120 µl or 200 µl
Format Magnetic beads
Main sample type Whole blood
Processing Automated on the QIAsymphony
Sample amount 2.5 ml
Technology Magnetic particles technology
Time per run 72 samples in 6 h 15 min
Yield 8–16 µg

You are not authorized to download the resource

Brochures & Guides
1
For collection of stable blood samples and purification of high-quality RNA for demanding downstream applications

 


Show details
Labware Documents
2
Application Notes
1
Typical total RNA yields from PAXgene Blood RNA Tubes processed with the QIAsymphony PAXgene Blood RNA Kit
Show details
Application/Protocol Documents
1
For purification of total RNA, including miRNA, from human whole blood stabilized in PAXgene Blood RNA Tubes using the QIAsymphony SP and QIAsymphony PAXgene Blood RNA Kit.

 


Show details