QIAsymphony DSP Virus/Pathogen Kit

适用于1–96个样本的病毒核酸或细菌/病原体DNA自动化纯化
  • 标准化操作流程带来的结果高度可重复性
  • 附带的条形码扫描功能可用于样本和试剂的全程追踪
  • 经优化的实验步骤可获得高产量的纯化核酸
  • 方便易用的创新性预装试剂条
QIAsymphony DSP Virus/Pathogen Kits系列试剂盒配套QIAsymphony SP仪器可为体外诊断实验所需并来源于多种样本的病毒核酸和细菌/病原体DNA提供自动化纯化方案。该类试剂盒可分为小量和中量提取两种规格,适用于体积从200 µl到1000 µl的起始样本。对于组织、FFPE组织、培养的细胞及细菌样本,手工操作的样本预处理过程是必要的。经优化的一系列操作步骤可帮助从样本中获得较高产量的纯化DNA,灵活的洗脱体积为下游多种应用所需的不同DNA浓度优化提供了可能。创新的预装试剂条则能够最大程度的减少了实验设置和手工操作的时间。附带的条形码扫描功能可用于试剂的全程追踪。
Product Product no. Cat. no. List price:
 
 
Show details
    varies
Can't order online?
To place an order via phone, email or for requesting a quote, please provide the product’s name, number and catalog number.
Product Cat. no. List price:
QIAsymphony DSP Virus/Pathogen Midi Kit
For 96 preps (1000 µl each): includes 2 reagent cartridges and enzyme racks and accessories
937055
Inquire
QIAsymphony DSP Virus/Pathogen Mini Kit
For 192 preps (200 µl each): includes 2 reagent cartridges and enzyme racks and accessories
937036
Inquire

QIAsymphony DSP Virus/Pathogen Kits可用于体外诊断。


  • Main Image Navi
QIAsymphony DSP Virus/Pathogen procedure|Schematic of the QIAsymphony SP principle|Prefilled, sealed reagent cartridges|CE-IVD-marked|In vitro diagnostic medical device.|
|The QIAsymphony SP processes a sample containing magnetic particles as follows: A magnetic rod protected by a rod cover enters a well containing the sample and attracts the magnetic particles. The magnetic rod cover is positioned above another well and the magnetic particles are released. The QIAsymphony SP uses a magnetic head containing an array of 24 magnetic rods, and can therefore process up to 24 samples simultaneously. Steps 1 and 2 are repeated several times during sample processing.||||
Principle
QIAsymphony DSP Virus/Pathogen Kits系列试剂盒可用于来源于血清、血浆或是脑脊液中的病毒核酸纯化,也适用于如呼吸道相关拭子、吸液、痰液以及灌洗液(BAL)或是尿液、泌尿拭子(宫颈和尿道)样本中的病毒核酸或细菌DNA纯化。该试剂盒还可用于从多种DNA、RNA病毒以及革兰氏阴性、阳性菌中进行核酸的纯化。

QIAsymphony DSP Virus/Pathogen Kits系列试剂盒将病毒或细菌核酸提取过程中硅胶膜法所具有的快速、高效优势同磁珠法所具有的操作便捷性进行了很好的结合(参见QIAsymphony SP原理示意图)。创新的即用型试剂预装条里已预装了纯化步骤所需的全部试剂,并且会由QIAsymphony SP仪器进行自动开封,最大程度地较少了环境污染的风险(参见预装封闭的试剂条)。经优化的实验操作步骤在获取高质量核酸的基础上还能高效的去除蛋白、核酸酶以及其他杂质。经QIAsymphony DSP Virus/Pathogen Kits系列试剂盒纯化得到的核酸可直接适用于下游多种应用(如PCR)。该试剂盒可灵活选择不同的洗脱体积。
Procedure

该纯化步骤是为确保对潜在可感染样本进行处理时的安全性和可重复性而设计的,分为4步:裂解、吸附、洗涤以及洗脱(参见QIAsymphony DSP Virus/Pathogen 提取步骤)。工作台可进行快速设置,可节省宝贵的时间。实验时只需简单的将来自不同或相同试剂盒的最多2个试剂条放置于试剂抽屉中即可,在一次仪器运行过程中对于最多96个样本可同时运行多种不同的纯化步骤。相关试剂会根据样本量进行配套使用,不会造成试剂浪费而达到最大程度的成本控制。

 

采用 QIAsymphony DSP Virus/Pathogen Kit 的实验方案
实验方案 试剂盒 样本材料 处理体积
无细胞体液200 QIAsymphony DSP Virus/Pathogen Mini Kit 血浆、血清和 CSF 200 µl
无细胞体液500 QIAsymphony DSP Virus/Pathogen Midi Kit 血浆、血清和 CSF 500 µl
无细胞体液1000 QIAsymphony DSP Virus/Pathogen Midi Kit 血浆、血清和 CSF 1000 µl
复杂混合样本200 QIAsymphony DSP Virus/Pathogen Mini Kit 呼吸道样本(灌洗液、干拭子、传输介质、呼出物、痰)和泌尿生殖系统的样品(尿液、传输介质) 200 µl
复杂混合样本400 QIAsymphony DSP Virus/Pathogen Midi Kit 呼吸道样本(灌洗液、干拭子、传输介质、呼出物、痰)和泌尿生殖系统的样品(尿液、传输介质) 400 µl
复杂混合样本800 QIAsymphony DSP Virus/Pathogen Midi Kit 呼吸道样本(灌洗液、干拭子、传输介质、呼出物、痰)和泌尿生殖系统的样品(尿液、传输介质) 800 µl
Applications
QIAsymphony DSP Virus/Pathogen Kits系列试剂盒纯化得到的高品质核酸可用于DNA、RNA病毒以及细菌病原体的灵敏检测。得到的产量是线性均一的,无论对于高滴度还是低滴度的病毒或是细菌都可以进行准确的定量分析。高品质的纯化核酸可直接适用于下游的所有应用,如测序或是诸如实时定量PCR、RT-PCR等较为灵敏的检测实验。

You are not authorized to download the resource

Labware Documents
2
Application/Protocol Documents
9
For use with software version 4.0
Show details
For use with software version 4.0
Show details
For use with software version 4.0
Show details
For use with software version 4.0
Show details
For use with software version 4.0
Show details
For use with software version 4.0
Show details
For use with software version 4.0
Show details
For use with software version 4.0
Show details
For use with software version 4.0
Show details