QIAquick Nucleotide Removal Kit

从酶促反应物中回收纯化多至10 μg寡核苷酸(17–40mers)和DNA片段(40 bp–10 kb)

  • 即用型DNA回收率高达95%
  • 流程快速、方便
  • 3个简单步骤即可回收多达10 kb DNA片段
  • 包含凝胶上样染料,方便样本分析

QIAquick Nucleotide Removal Kit提供离心柱、缓冲液和收集管,基于硅胶膜技术,纯化寡核苷酸和DNA。去除没有整合的核苷酸、盐和其他杂质。使用快速的结合、洗涤、洗脱过程使用30–50 μl洗脱体积即可纯化大于17 nt的寡核苷酸和40 bp–10 kb的DNA。该流程可在QIAcube全自动核酸纯化仪上全自动运行。

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Product Cat. no. List price:
QIAquick Nucleotide Removal Kit (50)
50 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)
28304
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QIAquick Nucleotide Removal Kit (250)
250 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)
28306
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The QIAquick Nucleotide Removal Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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QIAquick Nucleotide Removal procedure.|Complete removal of nucleotides from labeled oligos.|GelPilot Loading Dye.|Spin column handling options — B.|Spin column handling options — A.|
|Polyacrylamide gel analysis of radioactive labeling reactions before (b) and after (a) purification using the QIAquick Nucleotide Removal Kit are shown.|GelPilot Loading Dye contains three tracking dyes to facilitate optimization of DNA resolution.
|QIAcube.
|Microcentrifuge.
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Performance
QIAquick Nucleotide Removal操作流程去除核酸、酶、盐和DNA样本中的其他杂质(参见"Complete removal of nucleotides from labeled oligos")。使用微型离心机,可以纯化17mer–10 kb的DNA。小于50 µl的样本可以使用DyeEx Spin Kit进行纯化。
Principle

QIAquick Kits提供硅胶膜装置,在高盐度缓冲液中结合DNA,低盐度缓冲液或水中洗脱DNA。纯化过程可去除引物、核苷酸、酶、矿物油、盐、琼脂糖、溴化乙锭和DNA样品中的其他杂质。硅胶膜技术解决了松散树脂的常见问题和不便,特制的结合缓冲液针对特殊应用优化,促进特定片段范围内DNA的选择性吸附。

凝胶上样染料

为实现更快、更方便的样品处理和分析,已提供胶上样染料。GelPilot Loading Dye含有3种示踪染料(二甲苯蓝、溴酚蓝和orange G)便于优化琼脂糖凝胶跑胶时间,避免小DNA片段跑得过远 (参见 "GelPilot Loading Dye")。

Procedure

QIAquick系统使用简单的结合、洗涤、洗脱过程(参见"QIAquick Nucleotide Removal procedure")。结合缓冲液直接加入到样本中,然后将混合液加入到QIAquick离心柱中。在缓冲液提供的高盐度条件下,核酸吸附到硅胶膜上。洗涤杂质,使用小体积量的低盐缓冲液或水洗脱DNA,所得DNA可直接用于后续应用。

处理过程

QIAquick离心柱可提供两个方便的处理方法。离心柱可放在传统的台式微型离心机上。QIAquick Nucleotide Removal Kit以及其他的QIAGEN离心柱试剂盒可在QIAcube全自动核酸纯化仪器上全自动化运行,确保高产量和标准化的结果(参见"Spin column handling options A, and B")。

Applications

QIAquick系统纯化的DNA片段可直接用于各种应用,包括测序、微阵列分析、连接和转化、限制性酶切、标记和显微注射。

Feature
Specifications
Binding capacity 10 µg
Elution volume 30–50 µl
Format Tube
Fragment size 40 bp – 10 kb
Processing Manual
Recovery: oligonucleotides dsDNA Recovery: oligonucleotides, dsDNA
Removal <10mers 17–40mers dye terminator proteins Removal <10mers
Sample type: applications DNA, oligonucleotides: PCR reactions
Technology Silica technology

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Supplementary Protocols
1
QIAquick® Spin Columns can now be used on any vacuum manifold with luer connectors, for example, the QIAvac 6S or QIAvac 24 with QIAvac Luer Adapters. This protocol is designed for removal of primers <10 bases, enzymes, salts, and unincorporated nucleotides from biotin-, or DIG-labeled DNA fragments and oligonucleotides >17 nucleotides.
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