从凝胶或酶促反应物中回收纯化多至10 μg(70 bp–10 kb)DNA片段

  • 即用型DNA回收率高达95%
  • 流程快速、方便
  • 3个简单步骤即可回收多达10 kb DNA片段
  • 包含凝胶上样染料,方便样本分析

QIAquick Gel Extraction Kit包含离心柱、缓冲液和收集管,用于从凝胶(多至400 mg凝胶条)或酶促反应物中纯化DNA片段。通过简单快速的结合、洗涤、洗脱步骤,使用30–50 μl洗脱体积即可纯化得到70 bp–10 kb的DNA片段。通过内置的pH指示剂可容易确定DNA结合到离心柱的最佳pH值,整个过程可在QIAcube全自动核酸纯化仪上自动化运行。

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QIAquick Gel Extraction Kit (50)
For gel extraction or cleanup of 50 reactions: 50 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)
28704
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QIAquick Gel Extraction Kit (250)
For gel extraction or cleanup of 250 reactions: 250 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)
28706
Inquire
The QIAquick Gel Extraction Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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QIAquick and MinElute procedure.|High recoveries from gels.|GelPilot Loading Dye.|pH Indicator Dye.|Spin column handling options — D.|Sin column handling options — E.|Spin column handling options — C.|Spin column handling options — B.|Spin column handling options — A.|Reliable sequencing after gel extraction.|
The QIAquick and MinElute systems use a simple bind-wash-elute procedure with spin columns or a vacuum manifold.
|DNA fragments (sizes indicated) before extraction with the QIAquick Gel Extraction Kit and pooled after extraction are shown. Recoveries of approximately 80% are obtained for all fragment sizes [A] 1–5: before extraction; P: pooled after extraction. Samples were analyzed on a 1.5% agarose gel in TAE buffer. [B] 1–3: before extraction; P: pooled after extraction. Samples were analyzed on a 3.5% high-resolution agarose gel in TAE buffer. M: pTZ-HinfI markers.|GelPilot Loading Dye contains three tracking dyes to facilitate optimization of DNA resolution.

|pH indicator dye in the solubilization and binding buffer allows easy visual determination of optimal pH for DNA adsorption (pH ≤7.5). An incorrect binding-mixture pH can occur if the agarose gel electrophoresis buffer was frequently used or incorrectly prepared. In this case, the pH can be easily adjusted by addition of 10 µl 3 M sodium acetate, pH 5.0.
|QIAvac 24 plus.
|QIAcube.
|Manifold with luer connectors.
|QIAvac 24.
|Microcentrifuge.
|Sequence of a 2.7 kb PCR product extracted from a TAE agarose gel with the QIAquick Gel Extraction Kit is shown.
|
Performance
QIAquick Gel Extraction Kit能够去除样本中的核苷酸、酶、盐、琼脂糖、溴化乙锭和样本中的其他杂质,确保高达80%的DNA回收率(参见"High recoveries from gels")。使用离心机或者真空底座,可从1–24个样本中纯化得到70 bp到10 kb的DNA。纯化的DNA可用于测序等应用(参见"Reliable sequencing after gel extraction")。小于70 bp或者大于10 kb的DNA片段应该使用QIAEX II Gel Extraction System进行纯化。
Principle

QIAquick Kits基于硅胶膜技术,在高盐度缓冲液中结合DNA,应用低盐度缓冲液或水洗脱DNA。纯化过程去除引物、核苷酸、酶、矿物油、盐、琼脂糖、溴化乙锭和DNA样品中的其他杂质(参见 "High recoveries from gels")。硅胶膜技术避免了松散的树脂等常见问题和不便。特制的结合缓冲液经优化,促进特定片段范围内的DNA的吸附。

凝胶上样染色

为更快、更方便地处理样品和分析,已提供凝胶上样染料。GelPilot Loading Dye含有3种示踪染料(二甲苯蓝、溴酚蓝和orange G),便于优化琼脂糖凝胶跑样时间,避免DNA小片段跑得过远(参见"GelPilot Loading Dye")。

Procedure
QIAquick体系应用简化的结合、洗涤、洗脱操作流程(参见"QIAquick and MinElute procedure")。首先将胶碎片溶解在含有pH指示剂的缓冲液中,以此确定DNA结合的最佳pH值,然后将混合液装载到QIAquick离心柱上(参见"pH Indicator Dye")。在高盐度条件下核酸吸附到硅胶膜上,洗去杂质,应用低盐度缓冲液或水洗脱DNA,所得DNA可直接用于后续应用中。
处理过程

QIAquick离心柱提供两个方便的操作方法。离心柱可放在传统的台式微型离心机上或通过接头连接到任何真空底座上,如QIAvac 24 Plus和QIAvac Luer Adapters。QIAquick Gel Extraction Kit以及其他的QIAGEN离心柱试剂盒可在QIAcube全自动核酸纯化仪器上全自动化运行,确保高产量和标准化的结果(参见"Spin column handling options A, B, C, D, and E")。

Applications

QIAquick系统纯化的DNA片段可直接用于各种应用,包括测序、连接和转化、限制性酶切、标记、显微注射、PCR和体外转录。

Feature
Specifications
Binding capacity 10 µg
Elution volume 30–50 µl
Format Tube
Fragment size 70 bp – 10 kb
Processing Manual
Recovery: oligonucleotides dsDNA Recovery: dsDNA fragments
Removal <10mers 17–40mers dye terminator proteins Removal <10mers
Sample type: applications DNA: PCR reactions
Technology Silica technology

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