QIAquick 96 PCR Purification Kit

96孔板纯化96个PCR产物(多至10 μg),100 bp–10 kb

  • 即用型DNA回收率高达95%
  • 流程快速、方便
  • 3个简单步骤即可回收多达10 kb DNA片段

QIAquick 96 PCR Purification Kits含有96孔板、缓冲液和收集管,利用硅胶模纯化技术高通量纯化大于100 bp PCR产物。通过简单、快速的结合、洗涤、洗脱的流程纯化长达10 kb的DNA,洗脱体积为60–80 μl(最终洗脱体积为40–60 μl)。回收过程可在BioRobot工作站上使用QIAquick 96 PCR BioRobot Kit全自动运行。

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QIAquick 96 PCR Purification Kit (4)
For purification of 4 x 96 PCR reactions: 4 QIAquick 96 Plates, Buffers, Collection Microtubes (1.2 ml), Caps
28181
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QIAquick 96 PCR Purification Kit (24)
For purification of 24 x 96 PCR reactions: 24 QIAquick 96 Plates, Buffers, Collection Microtubes (1.2 ml), Caps
28183
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QIAquick 96 PCR BioRobot Kit (4)
For purification of 4 x 96 PCR products: 4 QIAquick 96 Plates, Reagents, Buffers, Collection Microtubes (1.2 ml) and Caps, 96-Well Microplates RB and Lids, Tape Pads
963141
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QIAquick 96 PCR Purification Kit are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.


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QIAquick 96 procedure.|Accurate sequencing.|
The QIAquick 96 Kit uses a bind-wash-elute procedure with QIAvac 96 or the BioRobot Systems.
|Dye-terminator sequencing of a 1 kb PCR product template was purified using the QIAquick 96 PCR Purification Kit and sequenced on an ABI PRISM 377XL DNA sequencer using an internal primer.
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Performance
QIAquick 96 PCR Kit提供快速、简单的方法,高通量纯化样本,回收率高达90%。QIAquick 96操作流程提供高纯度DNA适用于不同的下游应用(参见"Accurate sequencing")。采用QIAvac 96,可从1–4 x 96个样本中纯化得到100 bp–10 kb的DNA片段。
Principle

QIAquick 96 Kits包含一个硅胶膜,可在高盐缓冲液中结合DNA,并且在低盐缓冲液或水中洗脱。纯化过程去除引物、核苷酸、酶、矿物油、盐、琼脂糖、溴化乙锭和DNA样本中的其他杂质。硅胶膜技术结局了与树脂松动的问题和不便。优化特殊的结合缓冲液用于特殊应用,并且在特定的片段范围内,促进DNA的选择性吸附。

QIAquick 96操作流程平行纯化多达96个样本,并在QIAvac 96上采用高效真空驱动的纯化过程。

Procedure

QIAquick体系采用简单的结合、洗涤、洗脱操作流程(参见"QIAquick 96 procedure")。将结合缓冲液直接加到PCR样本或者其他酶试剂中,然后将混合液上样到96孔板上。在高盐的缓冲液中,核酸吸附到硅胶膜上。洗去杂质,并且用少量的低盐缓冲液或纯水洗脱纯DNA,为后续应用准备。

处理过程

QIAquick多孔模块在QIAvac底座上采用真空驱动的纯化过程处理。QIAquick 96 PCR Purification Kit需要使用QIAvac 96真空底座。产物回收过程可以使用QIAquick 96 PCR BioRobot Kit在BioRobot工作站上自动运行。

Applications

采用MinElute或者QIAquick体系纯化的DNA片段可即用于多种下游应用,包括测序、微阵列分析、连接和转化、限制性酶切、标记、显微注射、PCR和体外转录。

Feature
Specifications
Binding capacity 10 µg
Elution volume 60–80 µl
Format 96-well plate
Fragment size 100 bp – 10 kb
Processing Manual/automated
Recovery: oligonucleotides dsDNA Recovery: oligonucleotides, dsDNA
Removal <10mers 17–40mers dye terminator proteins Removal <40mers
Sample type: applications DNA, oligonucleotides: PCR reactions
Technology Silica technology

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Kit Handbooks
1
For rapid purification of multiple PCR products 
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