可纯化至多20 μg分子生物学级纯质粒DNA

  • 数分钟内可纯化得到即用型质粒DNA
  • 分子生物学级纯质粒DNA,重复性好
  • 针对高拷贝和低拷贝质粒载体提供不同的的实验方案
  • 依据高产量补充实验方案操作,可以获得更高产量

QIAprep Spin MiniPrep Kit用于纯化获得至多20 µg高纯度质粒或柯斯质粒DNA,得到的产物适用于常规分子生物级应用,如:荧光、放射性测序和克隆。依据高产量补充实验方案操作,可获得高达30 μg的高产量。

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QIAprep Spin Miniprep Kit (50)
For 50 high-purity plasmid minipreps: 50 QIAprep 2.0 Spin Columns, Reagents, Buffers, Collection Tubes (2 ml)
27104
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QIAprep Spin Miniprep Kit (250)
For 250 high-purity plasmid minipreps: 250 QIAprep 2.0 Spin Columns, Reagents, Buffers, Collection Tubes (2 ml)
27106
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The QIAprep Spin Miniprep Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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The QIAprep procedure.|Complete digestion with various restriction enzymes.|GelPilot Loading Dye|Spin column handling options — A.|Spin column handling options — C.|Spin column handling options — B|
|Restriction analysis of pBluescript DNA purified with the QIAprep Spin Miniprep Kit is shown. Digestion with the indicated enzymes (1–5 units) was carried out on 1 µg plasmid DNA. Markers: Lambda-HindIII.|The 3 tracking dyes enable easy optimization of gel electrophoresis times and monitoring of small DNA fragments.|Microcentrifuge.
|QIAcube.
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Performance

QIAprep Spin Miniprep Kit纯化得到多达20 ug分子生物级质粒DNA或科斯质粒DNA,适用于常规分子生物学级应用,如:PCR、测序和克隆。多功能的QIAprep离心柱可用于微离心机、真空装置或QIAcube全自动核酸纯化仪(参见"QIAprep Spin Column handling options AB, and C")。真空操作流程操作简单并可快速处理样本。QIAprep离心柱用QIAvac 24 plus或其它任何带有鲁尔接头的装置进行真空处理。QIAprep Spin Miniprep Kit目前也可在QIAcube全自动核酸纯化仪上全自动运行。

 
QIAprep Spin Miniprep Kit 规格
规格: 离心柱
纯化模块: QIAprep离心柱
吞吐量: 1–24个样本
制备时间: 30分钟内完成24个样本的制备
所需设备: 微离心机或真空装置;QIAcube全自动核酸纯化仪
裂解物清洗: 离心技术
柱容量: 800 µl
最小洗脱体积: 50 µl
高拷贝质粒培养容量: 1–5 ml
低拷贝质粒或科斯质粒培养容量: 1–10 ml

纯化得到的DNA可用于限制性酶切(参见"Complete digestion with various restriction enzymes")。 

Principle

QIAprep离心柱含独特的硅胶膜,在高浓度离液盐中可结合多达20 µg DNA,然后用少量低盐缓冲液洗脱。QIAprep膜技术去除了耗时的苯酚 氯仿抽提和乙醇沉淀,解决了树脂松散和悬浮液带来的问题与不便。从QIAprep离心柱洗脱得到的高纯度质粒DNA可直接使用,无需沉淀、浓缩或脱盐。

Procedure

QIAprep Kits纯化质粒DNA只需简单的结合-洗涤-洗脱的过程(参见"The QIAprep procedure")。首先,裂解细菌培养基,裂解物通过离心澄清。澄清后的细菌裂解液加到QIAprep模块中,质粒DNA吸附到硅胶膜上。洗去杂质后,用少量洗脱液或水洗脱,得到纯DNA。

除了从Escherichia coli纯化得到质粒外,QIAprep Kits还可以从Saccharomyces cerevisiaeBacillus subtilisAgrobacterium tumefaciens中纯化得到质粒 DNA。请联系QIAGEN技术专家或当地经销商获得相关应用的实验方案。

Applications

QIAprep Miniprep Kits可纯化得到的纯DNA适合多种应用,包括:

PCR
限制性酶切
连接和转化
测序 
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Feature
Specifications
Applications Fluorescent and radioactive sequencing (including capillary sequencing), ligation, cloning, transformation, etc.
Culture volume/starting material 1–10 ml culture volume
Elution volume 50 µl (minimal)
Plasmid type High-copy, low-copy, cosmid DNA
Processing Manual (vacuum or centrifugation)
Samples per run; throughput 1–24 samples per run
Technology Silica technology
Time per run or prep per run <30 minutes
Yield <20 µg

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Application/Protocol Documents
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