使用QIAGEN离心柱试剂盒,进行全自动样本制备

  • 对QIAGEN离心柱试剂盒,实现自动化处理
  • 全自动流程,无需手工操作
  • 全自动纯化DNA、RNA或蛋白
  • 自动化流程,更加节省时间
  • 标准化的结果,更高的工作效率

新型的QIAcube全自动核酸纯化仪采用先进的技术处理QIAGEN离心柱,将全自动、低通量样本制备无缝整合到您的实验流程中。无需更换纯化试剂,确保实验的快速启动,快速得到结果。所有纯化步骤全自动化,每批最多可处理12个样本。

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QIAcube System (110 V)
Robotic workstation for automated purification of DNA, RNA, or proteins using QIAGEN spin-column kits: includes installation and training, 1-year warranty on parts and labor
9001882
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QIAcube Priority Package (110 V)
Robotic workstation for automated purification of DNA, RNA, or proteins using QIAGEN spin-column kits: includes Priority Package with software, installation, training, 2-year warranty on parts and labor, and 2 preventive maintenance visits
9001883
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QIAcube Priority Package Plus (110 V)
Robotic workstation for automated purification of DNA, RNA, or proteins using QIAGEN spin-column kits: includes Priority Package Plus with software, installation, training, 3-year warranty on parts and labor, and 3 preventive maintenance visits
9001884
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QIAcube (230 V)
Robotic workstation for automated purification of DNA, RNA, or proteins using QIAGEN spin-column kits: includes 1-year warranty on parts and labor
9001293
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QIAcube System (230 V)
Robotic workstation for automated purification of DNA, RNA, or proteins using QIAGEN spin-column kits: includes installation and training, 1-year warranty on parts and labor
9001885
Inquire
QIAcube Priority Package (230 V)
Robotic workstation for automated purification of DNA, RNA, or proteins using QIAGEN spin-column kits: includes Priority Package with software, installation, training, 2-year warranty on parts and labor, and 2 preventive maintenance visits
9001886
Inquire
QIAcube Priority Package Plus (230 V)
Robotic workstation for automated purification of DNA, RNA, or proteins using QIAGEN spin-column kits: includes Priority Package Plus with software, installation, training, 3-year warranty on parts and labor, and 3 preventive maintenance visits
9001887
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Starter Pack, QIAcube
Pack includes: reagent bottle racks (3); rack labeling strips (8); 200 µl filter-tips (1024); 1000 µl filter-tips (2048); 30 ml reagent bottles (18); rotor adapters (240); rotor adapter holder; 1.5 ml elution tubes (240)
990395
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QIAcube全自动核酸纯化仪仅与QIAGEN提供的、在使用手册中说明可用于QIAcube全自动核酸纯化仪的试剂盒配套使用。


Reproducible DNA yields.|High recovery of PCR products.|DNA yields comparable to the manual procedure.|Consistent yields of RNA.|Automated QIAprep Mini Standard procedure.|High PCR performance.|Highly reproducible protein purification.|Comparison of manual and automated RNA purification.|Reliable sequencing results.|Red Dot Award.|Association for Laboratory Automation’s (ALA) New Product Award (NPA)|
Human blood was collected from 12 donors and treated with EDTA. Genomic DNA was purified from 200 µl blood using the QIAamp DNA Blood Mini Kit on the QIAcube. DNA was eluted in 200 µl. Yields of genomic DNA were determined by measuring the absorbance at 260 nm. The mean values of 3 independent runs are indicated.|PCR products (2 µg in a volume of 100 µl) were purified using the QIAquick PCR Purification Kit with the manual procedure or automated on the QIAcube. Percentage recovery was determined spectrophotometrically. Recovery of PCR products using the QIAcube was comparable to the manual procedure.|pCMVβ plasmid DNA was purified from 3 ml overnight cultures of E. coli (DH5α) grown in LB medium using the QIAprep Spin Miniprep Kit automated on the QIAcube or by using the manual procedure. DNA yields were determined spectrophotometrically. Automated DNA purification on the QIAcube gave comparable yields to the manual procedure.|RNA was purified from 1 x 106 Jurkat cells using the RNeasy Mini Kit on the QIAcube. RNA yields were consistent, demonstrating the efficiency and reproducibility of the automated procedure.|Using the QIAcube. Fully automated purification of nucleic acids or proteins using QIAGEN spin kits, such as QIAprep Mini Kits, on the QIAcube eliminates the need for tedious manual steps. Sample preparation on the QIAcube follows the same steps as the manual procedure (i.e., lyse, bind, wash, and elute). No change in purification chemistry is required.|Human blood was collected from 3 donors and treated with one of 3 anticoagulants: EDTA, citrate, or heparin. Genomic DNA was purified from 200 µl blood using the QIAamp DNA Blood Mini Kit on the QIAcube. DNA was eluted in 200 µl. Yields of genomic DNA were determined by measuring the absorbance at 260 nm. A 1.2 kb fragment of the single-copy Hugl gene was amplified using 1 µl, 5 µl, or 10 µl purified DNA in a final reaction volume of 25 µl. Amplification reactions were performed using HotStarTaq DNA Polymerase. A 5 µl aliquot of each PCR was run on a 1.5% agarose gel. M: 100 bp ladder; +: positive control; : negative control.|6xHis-tagged green fluorescent protein (GFP) was purified in duplicate from 5 ml E. coli cultures under native conditions using the Ni-NTA Spin Kit with the manual procedure or automated on the QIAcube. Proteins were visualized by Coomassie staining after SDS-PAGE. A 10 µl aliquot of eluate was loaded. M: marker.|RNA was purified using the RNeasy Mini Kit with the manual procedure or automated on the QIAcube. RNA was purified from 1 x 106 cells (RNeasy standard, RNeasy DNase digestion); 5 x 106 cells (RNeasy QIAshredder); 25 µg total RNA (RNeasy cleanup). Purified RNA was analyzed on the Agilent 2100 bioanalyzer. The high RNA Integrity Number (RIN) indicates the high quality of the RNA.  |pUC19 Plasmid DNA was purified from a 1.5 ml overnight culture of E. coli grown in LB medium using the QIAprep Spin Miniprep Kit on the QIAcube and sequenced using the BigDye Terminator v3.1 Cycle Sequencing Kit. Sequencing reactions were purified using the DyeEx 96 Kit and analyzed on an ABI PRISM 3700 DNA Analyzer.|QIAcube was given the design industry’s “Red Dot Award” recognizing the platform’s innovation, functionality, quality and ergonomics.|The QIAcube - QIAGEN’s revolutionary new platform for sample preparation - receives the Association for Laboratory Automation’s (ALA) New Product Award (NPA).|
Performance
高纯度质粒DNA

QIAcube全自动核酸纯化仪配合QIAprep Spin Miniprep Kit,全自动纯化多达20 µg的分子生物级质粒DNA。流程全自动,包括裂解步骤,获得的质粒DNA的产量与手工操作相当(参见''DNA yields comparable to the manual procedure'')。纯化的DNA适用于常规的分子生物学应用,如荧光测序、克隆和转染强壮细胞(参见''Reliable sequencing results'')。

高品质基因组DNA

配合QIAamp DNA Blood Kit,QIAcube全自动核酸纯化仪纯化获得重复高产的高纯度基因组DNA(参见''Reproducible DNA yields'')。即使采用大洗脱体积,使用QIAcube全自动核酸纯化仪纯化的DNA在敏感的PCR应用中表现良好,表明纯化获得高纯度的DNA(参见''High PCR performance'')。

有效纯化病毒核酸

QIAcube全自动核酸纯化仪配合QIAamp MinElute Virus Spin Kit,有效纯化病毒核酸。在评估批内和批间样品的污染风险中,QIAamp MinElute Virus Spin流程使用含有阴性和阳性血浆样本(常规DNA病毒1 x 108 IU/ml)交替放置的检测板,进行严密测试。所有阳性样本被检测到,阴性样本无应答(参见''No sample carryover detected'')。

未检测到样本的污染
1 2 3 4 5 6 7 8 9 10 1112
批1 19.5 X 19.4 X 18.8 X X 19.0 X 19.1 X 19.8
批2 X 19.1 X 18.9 X 18.8 18.7 X 19.2 X 18.9 X
批3 X X X X X X X X X X X
阴性和阳性血浆样本(常规DNA病毒1 x 108 IU/ml)交替放置,在QIAcube全自动核酸纯化仪上使用QIAamp MinElute Virus Spin Kit纯化,real-time RT-PCR分析。阳性样本的平均CT 值为19。CT > 45的样本视为阴性。在这些条件下,未检测到样本的污染。
高性能、高纯度RNA

与QIAcube全自动核酸纯化仪联用,RNeasy Mini Kit从动物细胞中有效自动纯化总RNA,获得持续高产的RNA(参见''Consistent yields of RNA'')。使用QIAcube全自动核酸纯化仪纯化获得RNA的质量与手工操作相当(参见''Comparison of automated and manual RNA purification'')。

高品质蛋白质 

QIAcube全自动核酸纯化仪配合Ni-NTA Spin Kit,可靠纯化高纯度6xHis-tagged蛋白(参见''Highly reproducible protein purification'')。纯化的蛋白适合于各种应用,包括:机理研究、用于确定三维结构的结晶化预研究、分析蛋白-蛋白和蛋白-DNA间的相互作用和免疫反应产生抗体。

有效纯化DNA片段

QIAcube全自动核酸纯化仪使DNA纯化更加容易。经验证的QIAquick技术,配合经优化的方案,可直接纯化100 bp–10 kb的双链或单链DNA片段。引物、核苷酸、酶和其他杂质都被有效去除,回收的DNA与手工操作相当(参见''High recovery of PCR products'')。

Principle
QIAcube全自动核酸纯化仪能够使用成熟的QIAGEN离心柱试剂盒连续纯化核酸和蛋白,免除了繁琐的手动操作。新型的QIAcube全自动核酸纯化仪控制所有组件,包括:离心机、加热震荡器、移液体系和自动抓取器。这样QIAcube全自动核酸纯化仪能够全自动处理超过40种QIAGEN离心柱试剂盒。

QIAcube全自动核酸纯化仪预先装入各种程序,用于纯化RNA、基因组DNA、质粒DNA、病毒核酸和蛋白,以及DNA和RNA的回收。所有扩展的标准程序均可免费下载。此外,我们也可为您量身设计程序,满足您特殊的应用要求。
Procedure

QIAcube全自动核酸纯化仪采用简单的裂解、结合、洗涤和洗脱流程,纯化获得高纯度的核酸和蛋白(参见flowchart)。超过40种QIAGEN离心柱试剂盒可在QIAcube全自动核酸纯化仪上自动处理。

前所未有的易用性

QIAcube 全自动核酸纯化仪前所未有的易用性。工作站使用方便,只需简单的几个步骤,即可开始运行。无外置电脑,节省实验室空间。内置触摸屏,方便地进行程序选择;界面清楚直观,程序中提供完善的指导信息,指导使用者进行工作站设置。为了提高易用性和处理的安全性,各种耗材和配件都带有方向标记,有助于减少误操作,确保正确安放。此外,程序开始前系统进行全面的自检,检查样本、试剂和耗材是否正确安放,以确保操作能顺利完成。

新型的专用于DNA和RNA制备的试剂盒

专用的QIAcube Kits进一步简化了在QIAcube全自动核酸纯化仪上的自动离心样本制备,更加方便。目前这些试剂盒适用于纯化RNA、基因组DNA 和病毒DNA。试剂盒中提供Rotor-adapters,预装有离心柱和洗脱管,更加方便和节省时间。而且,更加容易使用,最大限度的减少使用者的失误。这些专属试剂盒的容量专为在QIAcube全自动核酸纯化仪上纯化而量身设定,避免了试剂的浪费,而且管子亦无需手动填装。

Applications

QIAcube全自动核酸纯化仪非常非常适合科研实验室,以及药物研发、生物技术和生物医学研究实验室进行核酸和蛋白质的全自动提取 。

Feature
Specifications
Altitude Up to 2000 m
Centrifuge 12,000 x g maximum, swing-out rotor with 12 positions, maximum 45°
Humidity Relative humidity of 10-75% (noncondensing)
Instrument dimensions 650 x 620 x 570 mm
Kits designed for this instrument Most QIAGEN manual kits (e.g., QIAamp, QIAquick, QIAprep)
Operating temperature 18-28°C
Overvoltage category II
Pipetting system Syringe size 1 ml, pipetting range 5-900 µl
Place of operation For indoor use only
Pollution level 2
Power 100-120 V AC, 50-60 Hz, 650 VA (North America and Japan), 220-240 V AC, 50-60 Hz, 650 VA (Europe), mains supply voltage fluctuations are not to exceed 10% of nominal supply voltages
Processing Automated
Protocols/main application on this instrument DNA purification, RNA purification, protein purification
Samples per run; throughput 1–12 samples per run
Shaker Speed 100-2000 rpm, amplitude 2 mm, heating range of room temperature to 70°C, ramp-up time of <5 min from room temperature to 55°C (+/-)3°C)
Software QIAGEN protocols are preinstalled on the QIAcube or can be downloaded at www.qiagen.com/MyQIAcube
Storage temperature 15°C to 30°C in manufacturer's package
Technical data of the instrument 70.5 kg
Technology Depends on kit used
Touchscreen Transmissive TFT, 64 x 86 x 65 mm, white LED backlight, high brightness
Transportation temperature -25°C to 50°C in manufacturer's package

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Software User Guides
1
Download the documentation that describes how to update the QIAcube operating software and read through the procedure carefully. These instructions are the same for every update and are independent of the software version mentioned in the instruction document.
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