从血清和血浆中浓缩和纯化病毒DNA和RNA

  • 快速纯化高品质的即用型病毒DNA和RNA
  • 无需有机抽提或乙醇沉淀
  • 重复性好,产量高
  • 完全去除污染物和抑制剂
QIAamp UltraSens Virus Kit使用创新技术浓缩血清和血浆样品中的病毒核酸,接着使用经验证的QIAamp技术纯化核酸。样本起始体积可多至1 ml,向样本中添加创新型试剂纯化得到高浓缩核酸。试剂与核酸结合成复合物,可通过低速离心高度浓缩。
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QIAamp UltraSens Virus Kit (50)
For 50 viral nucleic acid preps: 50 QIAamp Mini Spin Columns, Proteinase K, carrier RNA, Collection Tubes (2 ml), buffers
53704
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QIAamp UltraSens Virus Kit (250)
For 250 viral nucleic acid preps: 250 QIAamp Mini Spin Columns, Proteinase K, carrier RNA, Collection Tubes (2 ml), buffers
53706
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The QIAamp UltraSens Virus Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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QIAamp UltraSens procedure.|High performance of purified nucleic acids in downstream analyses.|High performance in RT-qPCR.|
|RNA purified from a typical virus using the QIAamp UltraSens procedure was used in one-step RT-PCR with the LightCycler. geq/ml: genome equivalents per milliliter; : negative control; M: markers. Data kindly provided by Dr. Thomas Laue, Artus GmbH, Hamburg, Germany.|RNA purified from a typical virus using the QIAamp UltraSens procedure was used in quantitative, real-time RT-PCR with the LightCycler. geq/ml: genome equivalents per milliliter; : negative control; M: markers.|
Performance

QIAamp UltraSens Virus Kit适用于从多种病毒样本中纯化病毒核酸,也可高效分离血浆和血清中的胞外基因组DNA。制备的核酸品质卓越,适用于敏感的下游应用(参见"High performance in RT-PCR")。

纯化的DNA和RNA多种下游应用,包括:

PCR和real-time PCR
传染性疾病研究
Principle

DNA特异性结合到QIAamp硅胶膜上,污染物流出。通过两步高效洗涤完全去除二价阳离子和蛋白质等PCR抑制物,再用水或试剂盒中的缓冲液洗脱结合在离心柱上的纯DNA。

QIAamp技术从无细胞体液中纯化的病毒RNA和DNA,可即时用于PCR和印迹实验中。QIAamp样本制备技术完全得到验证认可。

Procedure

核酸浓缩通过将一种新型的试剂加入样本中来实现。试剂与核酸形成复合物,通过低速离心得到高浓缩核酸。该步骤可简便地对大体积样本进行核酸纯化。接着使用QIAamp硅胶膜技术纯化病毒核酸(参见"Procedure")。将裂解物上样到QIAamp离心柱,用洗涤缓冲液去除杂质,最后用纯水或低盐缓冲液洗脱得到高纯度的即用型DNA。

该流程无需超速离心或特殊的实验装置,在流程开始时进行内部控制,从而对纯化处理进行全程监测。

Applications

QIAamp UltraSens Virus Kit采用创新技术浓缩血浆和血清样本中的病毒核酸,并使用经验证的QIAamp技术纯化核酸。该流程可提高灵敏度,适用于病毒载量监测等要求较高病毒核酸回收率的应用。1小时内即可完成对1 ml血浆或血清样本的制备,核酸回收率为85%,洗脱体积为60 µl。

Feature
Specifications
Applications PCR, qPCR, real-time PCR.
Elution volume 60 µl
Format MinElute columns
Main sample type Serum, plasma
Processing Manual (centrifugation)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Viral DNA, viral RNA
Sample amount 1 ml
Technology Silica technology
Time per run or per prep 1 hour
Yield >85% recovery

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Kit Handbooks
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For highly efficient purification of viral RNA and DNA from 1 ml plasma and serum samples
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