QIAamp MinElute Virus Spin Kit

从血浆、血清和无细胞体液中同时纯化病毒DNA和RNA

  • 快速纯化高品质病毒DNA和RNA
  • 无需有机提取或乙醇沉淀
  • 重复性好,产量高
  • 完全去除污染物和抑制剂

QIAamp MinElute Virus Spin Kit使用快速离心柱简化了纯化病毒DNA和RNA的操作流程。QIAamp MinElute Virus Spin Kit的起始样品体积可多至0.2 ml,使用选择性结合的硅胶膜,具有20–150 μl的灵活洗脱体积。QIAamp MinElute Virus Spin可在QIAcube全自动核酸纯化仪上全自动运行。

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QIAamp MinElute Virus Spin Kit (50)
For 50 minipreps: 50 QIAamp MinElute Columns, QIAGEN Protease, carrier RNA, buffers, Collection Tubes (2 ml)
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The QIAamp MinElute Virus Spin Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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QIAamp MinElute Virus Spin procedure.|High sensitivity in PCR.|High sensitivity in RT-PCR.|
|The percentage of positive samples at low virus titers is shown. Nucleic acids from a DNA virus were purified from plasma using either the QIAamp MinElute Spin or Vacuum Kit and subjected to PCR. For the spin procedure, 95% probit values for 40 samples were 27.25 IU/ml and for the vacuum procedure, they were 9.30 IU/ml.|The percentage of positive samples at low virus titers is shown. Nucleic acids from an RNA virus were purified from plasma using either the QIAamp MinElute Spin or Vacuum Kit and subject to RT-PCR. For the spin procedure, 95% probit values for 32 samples were 22.84 IU/ml and for the vacuum procedure, they were 9.46 IU/ml.
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Performance

QIAamp MinElute Virus Spin Kit纯化的病毒核酸不含蛋白、核酸酶或其他污染物,适用于敏感的下游应用,如:PCR和RT-PCR(参见"High sensitivity in PCR"和"High sensitivity in RT-PCR")。

使用该试剂盒纯化的病毒核酸适用于多种下游应用,包括:

PCR和real-time PCR
传染性疾病研究
Principle

QIAamp MinElute Virus Spin Kit使用快速离心柱简化了从血浆、血清和无细胞体液中分离病毒RNA和DNA的过程。无需苯酚-氯仿抽提。核酸特异性结合到QIAamp MinElute硅胶膜上,污染物流出。通过两步高效洗涤可完全去除二价阳离子和蛋白质等PCR抑制剂,再用水或试剂盒中的缓冲液洗脱结合在离心柱上的纯核酸。QIAamp MinElute技术从血浆、血清和无细胞体液中纯化得到病毒RNA和DNA,可即时用于PCR和印迹实验中。

QIAamp MinElute Virus Spin Kit同时具备硅胶膜选择结合DNA的特性,以及20–150 µl的灵活洗脱体积。操作流程适用于血浆、血清和无细胞体液。QIAamp样本制备技术完全得到验证认可。

Procedure
试剂盒提供优化的缓冲液高效裂解样本,稳定核酸并促进DNA选择性结合到QIAamp MinElute硅胶膜上(参见"QIAamp MinElute Virus Spin procedure")。将添加乙醇的裂解液上样到QIAamp MinElute离心柱上。洗涤缓冲液用于去除杂质,用Buffer AVE洗脱的病毒核酸,可即时用于扩增反应或储存于–20ºC。纯化的核酸不含蛋白质、核酸酶或其他杂质。
Applications

QIAamp MinElute Virus Spin Kit使用成熟技术,可从多种样本中同时纯化病毒RNA和DNA,包括:

新鲜或冷冻的血浆
血清
其他无细胞体液
Feature
Specifications
Applications PCR, real-time PCR
Elution volume 20–150 µl
Format MinElute columns
Main sample type Serum, plasma
Processing Manual (centrifugation)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Viral DNA, viral RNA
Sample amount 200 µl
Technology Silica technology
Time per run or per prep <1 hour
Yield Varies

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