从液体培养基中纯化DNA

  • 从多种液体培养基中纯化DNA
  • 易于操作使用的真空流程,节省时间
  • 20–150 µl的灵活洗脱体积
  • 高效洗脱乙醇或其他污染物,制备高品质DNA

QIAamp MinElute Media Kit使用简便的真空流程从子宫拭子培养基等液体培养基中纯化核酸。可在QIAvac 24 Plus真空装置上快速操作QIAamp MinElute柱。QIAamp MinElute Media Kit可在QIAcube全自动核酸纯化仪上自动纯化DNA。

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QIAamp MinElute Media Kit
For 50 minipreps: 50 QIAamp MinElute Columns, QIAGEN Proteinase K, Carrier RNA, Buffers, Extension Tubes (3 ml), Collection Tubes (1.5 ml)
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The QIAamp MinElute Media Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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Performance

QIAamp MinElute Media Kit在90分钟内即可处理250 µl的液体转移培养基和贮存培养基样本。在QIAvac 24或QIAvac 24 Plus真空装置上使用QIAamp MinElute柱每批次可处理24个样本,DNA洗脱体积为20–150 µl 。

Principle

QIAamp MinElute Media Kit使用成熟的技术纯化核酸。该试剂盒的硅胶膜具有选择性结合的特性和20–150 µl的灵活洗脱体积,用于含有核酸的液体培养基,如子宫拭子培养基(如:PreservCyt 或 SurePath solution)。用Buffer AVE洗脱的核酸即时可用于扩增反应或贮存。纯化的核酸不含蛋白质、核酸酶和其他杂质。

Procedure

QIAamp MinElute Media流程包括4步(裂解、结合、洗涤和洗脱),在QIAvac 24 Plus真空装置上使用QIAamp MinElute柱进行操作。此流程可确保无样本间的交叉污染,对易污染样本的手动操作安全可靠。简便的QIAamp MinElute流程高度适用于同时处理多个样本,90分钟内即可从24个样本中制备纯的核酸。

Applications

QIAamp MinElute Media Kit用于从子宫拭子培养基等液体培养基中纯化DNA。该试剂盒可从多种培养基中纯化细胞、细菌和病毒核酸,包括:

含有乙醇的液体细胞培养基(如:PreservCyt或SurePath)
磷酸缓冲液体转运培养基(如:M4RT)
Feature
Specifications
Applications PCR, real-time PCR
Elution volume 20–150 µl
Format MinElute columns
Main sample type Liquid media
Processing Manual (vacuum)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Viral DNA and RNA, bacterial DNA and RNA, cellular DNA and RNA
Sample amount 250 µl
Technology Silica technology
Time per run or per prep <90 minutes (24 samples)
Yield Varies

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