用于从粪便中分离纯化DNA

  • 快速纯化得到高品质、即用型DNA
  • 无有机提取或乙醇沉淀
  • 重复性好,产量高
  • 彻底去除污染物和抑制物

QIAamp DNA Stool Mini Kit应用硅胶膜技术从新鲜或冷冻的人类粪便或其他含有高浓度PCR抑制剂的样品类型中纯化至多30 μg的基因组DNA、细菌、病毒或寄生虫DNA。独特的吸附树脂InhibitEX配合经优化的缓冲液可去除PCR抑制剂。方便的QIAamp离心柱纯化过程只需50分钟,QIAamp DNA Stool Mini Kit可在QIAcube全自动核酸纯化仪上自动纯化DNA。

Product Product no. Cat. no. List price:
 
 
Show details
    varies
Can't order online?
To place an order via phone, email or for requesting a quote, please provide the product’s name, number and catalog number.
Product Cat. no. List price:
QIAamp DNA Stool Mini Kit (50)
For 50 DNA preps: 50 QIAamp Mini Spin Columns, QIAGEN Proteinase K, InhibitEX tablets, Buffers, Collection Tubes (2 ml)
51504
Inquire
The QIAamp DNA Stool Mini Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
  • Main Image Navi
QIAamp DNA Stool Mini procedure.|Removal of PCR inhibitors.|
|DNA was purified from 19 stool samples using [A] a conventional silica-based purification technique and [B] the QIAamp DNA Stool Mini Kit. To show whether inhibitors were present in the purified eluates, 5 µl of each eluate was added to PCR mixes with a template of 10 pg plasmid containing a GFP gene. Amplification of the GFP gene was successful in the presence of all QIAamp eluates whereas only 2 amplification reactions were successful in the presence of eluates prepared using the conventional technique containing the green fluorescent protein. M: markers; C: positive PCR control.|
Performance

QIAamp DNA Stool Mini Kit可从新鲜或者冰冻的人类粪便或其他含有高浓度PCR反应抑制剂的样本中快速纯化DNA。一般最多可处理220 mg的粪便,如需处理更大样本量,则需要更多地ASL缓冲液。50分钟内即可在200 µl的洗脱液中纯化得到10–30 µg的DNA。纯化的DNA长度可达50 kb。该长度的DNA变性完全,并具有最高的扩增效率。高纯度的DNA可直接用于下游扩增反应(参见"Removal of PCR inhibitors")。

使用QIAamp DNA Stool Mini Kit纯化的DNA可用于多种下游反应,包括PCR、real-time PCR、传染病研究和筛查。

Principle

DNA特异性结合到QIAamp硅胶模上,污染物流出。无需苯酚氯仿抽提。一种独特的吸附性树脂InhibitEX和经优化的缓冲液相结合,可去除PCR抑制剂。蛋白酶K可实现完全裂解,确保从粪便中获得高产量的各种常见DNA,如肠上皮细胞、细菌、病毒和其他肠胃病原体的DNA。

只需50分钟即可得到高纯度DNA,并可直接用于下游扩增实验。QIAamp样本制备技术已被完全认证。

Procedure
QIAamp DNA Stool Mini Kit采用快速的离心柱操作,简化了从粪便样本中分离DNA的步骤(参见"Procedure")。优化的缓冲液和酶裂解样本,稳定核酸,促使DNA选择性吸附到QIAamp硅胶模上。加入乙醇,然后将裂解产物上样到QIAamp离心柱上。InhibitEX和优化的缓冲液共同作用,去除PCR反应抑制剂。蛋白酶K可确保各种粪便中常见DNA的高产量。残留的杂质通过两次洗涤被高效去除。之后使用低盐缓冲液洗脱DNA,产物可即用于下游扩增实验。
Applications

QIAamp DNA Stool Mini Kit适用于从以下样本类型中分离纯化基因组DNA、细菌、病毒和寄生虫DNA:

新鲜粪便
冷冻粪便
其他含有高浓度PCR反应抑制剂的样本类型
Feature
Specifications
Applications PCR, blotting
Elution volume 200 µl
Format Spin column
Main sample type Stool samples
Processing Manual (centrifugation or vacuum)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Genomic DNA, bacterial DNA, parasite DNA, viral DNA
Sample amount 220 mg
Technology Silica technology
Time per run or per prep 50 minutes
Yield 10–30 µg

You are not authorized to download the resource

Kit Handbooks
2
For DNA purification from stool samples. Version June 2012
Show details
For DNA purification from stool samples
Show details
User-Developed Protocols
2
The protocol describes the preservation and concentration of stool samples (in preparation for microscopic examination for intestinal parasites), using two systems from Meridian Biosciences, followed by isolation of DNA from the stool samples for pathogen detection using the QIAamp DNA Stool Mini Kit.
Show details
As starting material, 5 g soil was mixed with different amounts of Bacillus subtilis cells. Sensitivity was 5 x 103 cells/5g soil.
Show details
References
0