从血液和相关体液中纯化基因组DNA线粒体DNA或病毒DNA,最高可达12 μg
  • 快速纯化高品质、即用型DNA
  • 无需有机提取或乙醇沉淀
  • 重复性好,产量高
  • 完全去除污染物和抑制剂

QIAamp DNA Blood Mini Kit应用硅胶膜技术,从多达200 μl新鲜或冷冻的人全血样本中纯化DNA。QIAamp Mini离心柱可在离心机或真空装置上使用。QIAamp DNA Blood Mini Kit可在QIAcube全自动核酸纯化仪自动纯化DNA。

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QIAamp DNA Blood Mini Kit (50)
For 50 DNA minipreps: 50 QIAamp Mini Spin Columns, QIAGEN Protease, Reagents, Buffers, Collection Tubes (2 ml)
51104
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QIAamp DNA Blood Mini Kit (250)
For 250 DNA minipreps: 250 QIAamp Mini Spin Columns, QIAGEN Protease, Reagents, Buffers, Collection Tubes (2 ml)
51106
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QIAamp DNA Blood Mini Kit are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.


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QIAamp spin column procedure.|Apoptotic banding in stored blood.|Long-range PCR.|Paternity testing by RFLP analysis.|The QIAcube.|
Optimized buffers lyse samples, stabilize nucleic acids, and enhance selective DNA adsorption to the QIAamp membrane. Alcohol is added and lysates loaded onto the QIAamp spin column. Wash buffers are used to remove impurities and pure, ready-to-use DNA is then eluted in water or low-salt buffer. The entire process requires only 20 minutes of handling time (lysis times differ according to the sample source).|Genomic DNA from 8 blood samples stored at 4°C for 1 week. DNA was purified using the QIAamp DNA Blood Mini Kit. When blood is stored at 4°C, the DNA is rapidly degraded due to apoptosis; the resulting apoptotic banding pattern can clearly be seen in these samples. M1: lambda-HindIII; M2:100 bp ladder.|Amplification of a 10 kb fragment of the human ALDH1 gene from genomic DNA isolated from blood. DNA was purified using conventional methods (Phenol) or the QIAamp DNA Blood Maxi Kit (QIAamp). PCR products were sequenced directly. M: 1 kb ladder.|Three paternity cases tested with DNA purified from blood samples with the QIAamp DNA Blood Mini Kit. 1: mother; 2: child; 3: alleged father; 4: child + alleged father. M: markers. Each lane had 3 µg DNA loaded. Outcome: A: alleged father excluded; B and C: alleged father confirmed. (Data kindly provided by J. James, Gene Proof Technologies, Nashville, TN, USA.)||
Performance

QIAamp DNA Blood Kits根据样本存储条件和新鲜程度不同,可纯化获得大小为200 bp–50 kb的DNA(参见"Apoptotic banding in stored blood")。纯化的DNA适用于长片段PCR扩增(参见"Long-range PCR")和RFLP分析等等,如亲子鉴定(参见"Paternity testing by RFLP analysis")。

Principle

无需苯酚-氯仿抽提。DNA特异性结合到QIAamp硅胶膜上,污染物流走。PCR抑制剂,如:二价阳离子和蛋白,可通过两步有效的洗涤被完全去除,结合在离心柱上的纯核酸可用水或试剂盒中的缓冲液洗脱。QIAamp DNA Blood技术从血液或相关体液纯化得到的基因组、线粒体或病毒DNA,可即用于PCR和印迹实验中。

Procedure

QIAamp DNA Blood Mini Kit采用快速离心柱或真空操作简化了从血液或相关体液中分离DNA的流程(参见"procedure")。该试剂盒可处理带有EDTA、柠檬酸盐和肝素等常规抗凝剂的新鲜或冷冻的全血样本。 QIAamp DNA Blood Mini Kit可处理多达200 µl的样本,制备时间20–40分钟。200 µl健康全血可获得4–12 µg DNA,洗脱体积50–200 µl。QIAamp DNA Blood Mini Kit的操作可自动化(参见"QIAcube")。

QIAamp样本制备技术是完全经过验证的。

真空处理

使用QIAamp DNA Blood Mini Kit,血液样本可用真空处理而无需离心,可更快速、更方便地纯化DNA。使用VacValves和VacConnectors装置,QIAamp Mini离心柱可配合QIAvac 24 manifold真空底座使用。在样本流速显著不同时,需使用VacValves以确保一致的真空条件。一次性的VacConnectors用于避免交叉污染。配合VacConnectors,也可使QIAamp离心操作在带有QIAvac Luer Adapters的QIAvac 6S上进行。

Applications

QIAamp DNA Blood Mini Kit使用经验证的QIAamp技术,采用带有2.0 ml收集管的Mini离心柱从不同来源的样本中纯化DNA。样本来源包括:

新鲜和冷冻的全血或白膜层
血浆或血清
骨髓
淋巴细胞
血小板
体液
Feature
Specifications
Applications PCR, long-range PCR, Southern blotting
Elution volume 50–200 µl
Format Spin column
Main sample type Whole blood, body fluids
Processing Manual (centrifugation or vacuum)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Genomic DNA, mitochondrial DNA, viral DNA
Sample amount 1–200 µl
Technology Silica technology
Time per run or per prep 20–40 minutes
Yield 4–12 µg

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User-Developed Protocols
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Buffer AS (a special stabilizing reagent available from QIAGEN on request) allows storage of blood samples for up to 12 weeks at temperatures up to 37°C before preparation of DNA with QIAamp® Kits, without any significant reduction in DNA yield or quality. Storage at lower temperatures will extend the potential storage time.
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