PyroMark Buffers and Solutions

采用焦磷酸测序技术,优化模板DNA的制备和分析

    使用PyroMark真空工作站可快速、直接的从PCR扩增子生成焦磷酸测序模板,这些缓冲液和溶液专为此而设计。PyroMark Binding Buffer可促进亚硫酸氢盐转化后的PCR扩增子与覆盖链酶亲和素的琼脂糖凝胶微球结合。随后将这些微球固定在工作站的真空仪器上。PyroMark Denaturation Solution可温和的从作为焦磷酸测序模板的生物素标记链上分离其互补链,PyroMark Wash Buffer确保只有生物素标记的单链DNA结合在真空容器中。最后,当单链DNA释放到含焦磷酸测序引物的反应容器时,PyroMark Annealing Buffer可加强引物和模板的结合。

    Product Product no. Cat. no. List price:
     
     
    Show details
        varies
    Can't order online?
    To place an order via phone, email or for requesting a quote, please provide the product’s name, number and catalog number.
    Product Cat. no. List price:
    PyroMark Annealing Buffer
    For annealing of sequencing primer to DNA template
    979009
    Inquire
    PyroMark Binding Buffer (200 ml)
    For binding of biotinylated PCR product to Sepharose beads
    979006
    Inquire
    PyroMark Denaturation Sol. (500 ml)
    For denaturation of double-stranded PCR product into single-stranded DNA template
    979007
    Inquire
    PyroMark Wash Buffer (conc., 200 ml)
    For use with the PyroMark Q24 Vacuum Workstation and PyroMark Q96 Vacuum Workstation to wash and neutralize the immobilized DNA
    979008
    Inquire

    PyroMark Buffers and Solutions are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.


    You are not authorized to download the resource