从酶促反应物中回收多至5 μg DNA(70 bp到4 kb)

  • 非常小的洗脱体积
  • 回收速度快,操作容易
  • 回收率高,重复性好
  • 含有凝胶上样染料,方便样本分析

MinElute Reaction Cleanup Kit含有离心柱、缓冲液和收集管,基于硅胶膜技术,用于从酶促反应物中纯化70 bp到4 kb的DNA片段。特殊设计的离心柱可将DNA洗脱至非常小的体积(10 μl),获得高产、高度浓缩的DNA。通过内置的pH指示剂可容易确定DNA结合到离心柱的最佳pH值。实验可在QIAcube全自动核酸纯化仪上全自动运行。应用MinElute System纯化的DNA可即用于各种应用,包括测序、微阵列分析、连接和转化、限制性酶切、标记、显微注射、PCR和体外转录。

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MinElute Reaction Cleanup Kit (50)
50 MinElute Spin Columns, Buffers, Collection Tubes (2&nbsp:ml)
28204
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MinElute Reaction Cleanup Kit (250)
250 MinElute Spin Columns, Buffers, Collection Tubes (2 ml)
28206
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The MinElute Reaction Cleanup Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
  • Main Image Navi
MinElute procedure.|GelPilot Loading Dye.|MinElute membrane assembly.|pH Indicator Dye.|Spin column handling options — D.|Spin column handling options — E.|Spin column handling options — C.|Spin column handling options — B.|Spin column handling options — A.|
This simple bind–wash–elute procedure ensures greater convenience.


|GelPilot Loading Dye contains three tracking dyes to facilitate optimization of DNA resolution.

|MinElute spin column in cross section, showing the unique membrane assembly (utility model pending).
|pH indicator dye in the solubilization and binding buffer allows easy visual determination of optimal pH for DNA adsorption (pH ≤7.5). An incorrect binding-mixture pH can occur if the agarose gel electrophoresis buffer was frequently used or incorrectly prepared. In this case, the pH can be easily adjusted by addition of 10 µl 3 M sodium acetate, pH 5.0.
|QIAvac 24 plus.
|QIAcube.
|Manifold with luer connectors.
|QIAvac 24.
|Microcentrifuge.
|
Performance

MinElute Reaction Cleanup Kit可从酶促反应物中纯化多达5 μg的DNA(70 bp–4 kb),获得的高产量DNA适用于各种应用。该试剂盒提供纯化酶促反应物所需的离心管。使用离心机或真空装置可快速获得高度浓缩的DNA片段(70 bp–4 kb)。(大于4 kb的DNA片段使用QIAquick System纯化。)

MinElute Reaction Cleanup Kit完全清除的酶举例
酶的分子量(kDa)
DNA聚合酶I 109
Klenow片段 62
小牛肠碱性磷酸酶 69
T4 DNA连接酶 55
T4聚核苷酸激酶 35
末端转移酶 32
DNase I 31
限制性内切酶 不定
Principle

MinElute Kits采用硅胶膜式纯化柱,在高盐条件下结合DNA,低盐或水可洗脱DNA。纯化过程去除引物、核苷酸、酶、矿物油、盐、琼脂糖、溴化乙锭和DNA样品中的其他杂质。硅胶膜技术避免了松散树脂和悬液状态的问题及不方便性。经优化的特制结合缓冲液,专用于选择性吸附特定大小范围内的DNA分子。

凝胶上样染料

为更快速、更方便地进行分析,提供上样染料。GelPilot Loading Dye含有3种示踪染料(xylene cyanol、bromophenol blue和orange G),便于优化凝胶运行时间,避免小片段DNA跑得过远(参见"GelPilot Loading Dye")。

Procedure

MinElute体系应用简单的结合-洗涤-洗脱步骤(参见"MinElute procedure")。直接将结合缓冲液加入PCR样品或其他酶反应中,然后将混合液装载到MinElute离心柱上。结合缓冲液含有pH指示剂,可方便地确定DNA结合的最佳pH值(参见pH Indicator Dye)。在高盐条件下,核酸吸附在硅胶膜上。洗去杂质并用少量的低盐缓冲液或水洗脱DNA,可即用于各种下游应用。

操作

MinElute离心柱有两种方便的处理方式(参见"MinElute procedure")。可可将离心柱放入传统的微型离心机或通过适配器连接到任何真空装置上,诸如通过QIAvac Luer Adapters连接到QIAvac 24 Plus或QIAvac 6S,也可在QIAcube全自动核酸纯化仪上全自动进行,确保高产量和标准化结果。

Applications

MinElute系列产品纯化的DNA片段可即用于各种应用,包括:

  • 测序
  • 微阵列分析
  • 连接和转化
  • 限制性酶切
  • 标记
Feature
Specifications
Binding capacity 5 µg
Elution volume 10 µl
Format Tube
Fragment size 70 bp – 4 kb
Processing Manual
Recovery: oligonucleotides dsDNA Recovery: oligonucleotides, dsDNA
Removal <10mers 17–40mers dye terminator proteins Removal <40mers
Sample type: applications DNA, oligonucleotides: Enzymatic reactions
Technology Silica technology

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Kit Handbooks
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For MinElute PCR Purification Kit, MinElute Gel Extraction Kit, MinElute Reaction Cleanup Kit
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