MinElute 96 UF PCR Purification Kit

使用超滤方法高通量纯化多至15 μg PCR产物(长度小于100 bp)

  • 非常小的洗脱体积
  • 纯化速度快,操作容易
  • 回收率高,重复性好

MinElute 96 UF PCR Purification Kit基于超滤技术,使用96孔板,用于>100 bp的PCR产物的高通量纯化。特殊设计的板子可将DNA洗脱至非常小的体积(20 μl),获得高产、高度浓缩的DNA。该流程可在BioRobot Universal System上自动化进行。使用MinElute体系纯化的DNA片段可直接用于各种应用,包括:测序、微芯片分析、连接和转化、限制性酶切、标记、显微注射、PCR和体外转录。MinElute 96 UF PCR Purification Kit需使用QIAvac Multiwell真空装置(货号:9014579)。

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MinElute 96 UF PCR Purification Kit (4)
4 MinElute 96 UF PCR Purification Plates
28051
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MinElute 96 UF PCR Purification Kit (24)
24 MinElute 96 UF PCR Purification Plates
28053
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The MinElute 96 UF PCR Purification Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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MinElute 96 UF PCR Purification procedure.|Highly concentrated DNA eluates.|
Using the manual procedure, 96 samples are prepared in 18 minutes.
|Efficient purification of concentrated DNA was achieved using the MinElute 96 UF PCR Purification Kit. DNA fragments (100 bp and 500 bp) were purified using either MinElute 96 UF PCR Purification Plates (QIAGEN) or ultrafiltration-based kits from other suppliers (Supplier MIII and Supplier M). Aliquots (5 μl) of the eluate were analyzed by agarose gel electrophoresis. M: markers.|
Performance
MinElute 96 UF PCR Purification Kit使用96孔板高通量纯化PCR产物。在BioRobot 3000(已停产)、BioRobot 8000或BioRobot Universal System上全自动处理1–8个96孔板,或在QIAvac Multiwell Manifold(货号:9014579,需合适的真空管,如QIAvac Multiwell管,建议使用孔板振荡器)上处理1个96孔板。纯化>100 bp的DNA的回收率为80–95%。使用少量洗脱液(人工操作需20 µl,如使用BioRobot工作站则需30 µl)即可将纯化的PCR产物从膜表面洗脱下来,得到高浓度DNA(参见Highly concentrated DNA eluates)用于各种下游应用。无论是人工操作还是全自动流程都可处理多达150 µl的PCR产物。
Principle
QIAGEN MinElute 96 UF PCR Purification Kit设计独特,结合了多孔技术的便利性和超滤膜的高效分离特性。使用孔板可对>100 bp的双链DNA片段进行快速高效回收,并除去引物(<20mers)、未整合的核苷酸链和盐。
Procedure
MinElute 96 UF PCR Purification Kits运用超滤技术,在真空环境下使用QIAvac纯化PCR产物(参见"MinElute 96 UF PCR Purification procedure")。在真空条件下,将PCR产物上样超滤板的孔中。引物、盐和未整合的核苷酸链等小分子通过滤膜,而大于100 bp的PCR产物留下来。水、DMSO或缓冲液如SSC等可以作为洗脱液。MinElute 96 UF PCR Purification操作流程可在BioRobot 3000(已停产)、BioRobot 8000、或BioRobot Universal System上全自动进行。
Applications

MinElute 96 UF系统纯化得到的DNA片段可直接用于各种应用,包括:

  • 测序
  • 微阵列分析
  • 连接和转化
  • 限制性酶切
  • 标记
Feature
Specifications
Binding capacity <150 µl
Elution volume 20–30µl
Format 96-well plate
Fragment size >100 bp
Processing Manual/automated
Recovery: oligonucleotides dsDNA Recovery: oligonucleotides, dsDNA
Removal <10mers 17–40mers dye terminator proteins Removal <20mers
Sample type: applications DNA, oligonucleotides: PCR reactions
Technology Ultrafiltration

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Kit Handbooks
1
For efficient, high-throughput purification of PCR products
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Safety Data Sheets
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References
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