mericon Pathogen Detection Assays

用于对食品及饲料中的病原体DNA进行real-time PCR检测

  • 对多种病原体进行高灵敏度、高特异性检测
  • 对所有目标待测物的检测实验方案高效、一致
  • 另有专用的细菌DNA纯化试剂盒
  • Rotor-Gene Q实时荧光定量PCR分析仪上运行时间为73分钟
  • 有PCR认证,无需额外的花费

mericon Pathogen Detection Assays适用于检测食品、饲料及药品中的细菌病原体DNA。所有的试剂盒都使用相同的基于real-time PCR的实验方案。mericon Pathogen Detection Assays是QIAGEN食品检测生产线中的一部分,此外还有专门用于成分鉴定及转基因生物DNA检测的样品制备及分析试剂盒。mericon试剂盒在Rotor-Gene Q实时荧光定量PCR分析仪上具有出色表现,经验证也可用于其他PCR仪。

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mericon Campylobacter spp Kit (24)
For 24 reactions: PCR Assay Campylobacter spp, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution
290033
Inquire
mericon Campylobacter spp Kit (96)
For 96 reactions: PCR Assay Campylobacter spp, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution
290035
Inquire
mericon Campylobacter triple Kit (24)
For 24 reactions: PCR Assay Campylobacter triple, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution
290043
Inquire
mericon Campylobacter triple Kit (96)
For 96 reactions: PCR Assay Campylobacter triple, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution
290045
Inquire
mericon Cronobacter spp Kit (24)
For 24 reactions: PCR Assay Cronobacter spp, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution
290063
Inquire
mericon Cronobacter spp Kit (96)
For 96 reactions: PCR Assay Cronobacter spp, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution
290065
Inquire
mericon E. coli O157 Screen Plus Kit (24)
For 24 reactions: PCR Assay E. coli O157 Screen Plus, Internal Control, Multiplex PCR Master Mix, RNase-Free Water
290403
Inquire
mericon E. coli O157 Screen Plus Kit (96)
For 96 reactions: PCR Assay E. coli O157 Screen Plus, Internal Control, Multiplex PCR Master Mix, RNase-Free Water
290405
Inquire
mericon E. coli STEC O-Type Kit (24)
For 24 reactions: PCR Assay E. coli O157 STEC O-Type, Internal Control, Multiplex PCR Master Mix, RNase-Free Water
290233
Inquire
mericon Listeria spp Kit (24)
For 24 reactions: PCR Assay Listeria spp, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution
290123
Inquire
mericon Listeria spp Kit (96)
For 96 reactions: PCR Assay Listeria spp, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution
290125
Inquire
mericon L. monocytogenes Kit (24)
For 24 reactions: PCR Assay L. monocytogenes, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution
290023
Inquire
mericon L. monocytogenes Kit (96)
For 96 reactions: PCR Assay L. monocytogenes, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution
290025
Inquire
mericon S. aureus Kit (24)
For 24 reactions: PCR Assay S. aureus, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution
290073
Inquire
mericon Salmonella spp Kit (24)
For 24 reactions: PCR Assay Salmonella spp, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution
290013
Inquire
mericon Salmonella spp Kit (96)
For 96 reactions: PCR Assay Salmonella spp, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution
290015
Inquire
mericon Shigella spp Kit (24)
For 24 reactions: PCR Assay Shigella spp, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution
290103
Inquire
mericon Shigella spp Kit (96)
For 96 reactions: PCR Assay Shigella spp, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution
290105
Inquire
mericon VTEC stx 1/2 Kit (24)
For 24 reactions: PCR Assay VTEC stx 1/2, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution
290053
Inquire
mericon VTEC stx 1/2 Kit (96)
For 96 reactions: PCR Assay VTEC stx 1/2, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution
290055
Inquire
mericon Y. enterocolitica Kit (24)
For 24 reactions: PCR Assay Y. enterocolitica, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution
290113
Inquire
mericon Y. enterocolitica Kit (96)
For 96 reactions: PCR Assay Y. enterocolitica, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution
290115
Inquire
mericon Quant Legionella spp Kit

For 96 reactions: PCR Assay Quant Legionella spp, Internal Control, Standard DNA, Quant Control DNA, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution

290085
Inquire
mericon Quant L. pneumophila Kit (96)

For 96 reactions: PCR Assay Quant L. pneumophila, Internal Control, Standard DNA, Quant Control DNA, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution

290095
Inquire
mericon Pathogen Detection Assays are intended for molecular biology applications in food, animal feed, and pharmaceutical product testing. These products are not intended for the diagnosis, prevention, or treatment of a disease.
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Performance
mericon Campylobacter spp Kit

mericon Campylobacter spp Kit用于特异性检测来源丰富的食品、饲料样本中的几种Campylobacter亚种。该试剂盒能检测低至10拷贝的Campylobacter DNA并对该种属病原体表现出高度特异性。在一次实验中使用2500拷贝的待测DNA未发现与其他病原体有交叉反应性(参见"mericon Campylobacter spp Kit交叉反应研究结果")。

mericon Campylobacter triple Kit

mericon Campylobacter triple Kit用于特异性检测来源丰富的食品、饲料样本中的Campylobacter jejuni、C. coliC. laridis。该试剂盒能检测低至10拷贝的C. jejuni、C. coliC. laridis DNA并表现出高度特异性。在一次实验中使用2500拷贝的待测DNA未发现与其他病原体有交叉反应性 (参见"mericon Campylobacter triple Kit交叉反应研究结果")。

mericon Cronobacter spp Kit

mericon Cronobacter spp Kit用于特异性检测来源丰富的食品、饲料样本中的Chronobacter亚种。由于最近分类学有所变化,根据欧盟委员会的调整(365/2010)用名称Cronobacter spp代替了Enterobacter sakazakii。该试剂盒能检测低至10拷贝的Cronobacter DNA并表现出高度特异性。在一次实验中使用2500拷贝的待测DNA未发现与其他病原体有交叉反应性(参见"mericon Cronobacter spp Kit交叉反应研究结果")。

mericon Legionella spp Kit

mericon Legionella spp Kit用于特异性检测过滤后的水中及来源丰富的食品、饲料样本中的几种Legionella亚种。该试剂盒能检测低至10拷贝的Legionella DNA并对该种属病原体表现出高度特异性。在一次实验中使用2500拷贝的待测DNA未发现与其他病原体有交叉反应性 (参见"mericon Legionella spp Kit交叉反应研究结果")。

mericon L. pneumophila Kit

mericon L. pneumophila Kit用于特异性检测过滤后的水中的Legionella pneumophila。该试剂盒能检测低至10拷贝的L. pneumophila DNA并表现出高度特异性。在一次实验中使用2500拷贝的待测DNA未发现与其他病原体有交叉反应性 (参见"mericon L. pneumophila Kit交叉反应研究结果")。

mericon Listeria spp Kit

mericon Listeria spp Kit用于特异性检测来源丰富的食品、饲料样本中的几种Listeria亚种。该试剂盒能检测低至10拷贝的Listeria DNA并对该种属病原体表现出高度特异性。在一次实验中使用2500拷贝的待测DNA未发现与其他病原体有交叉反应性(参见"mericon Listeria spp Kit交叉反应研究结果")。

mericon L. monocytogenes Kit

mericonL. monocytogenes Kit用于特异性检测来源丰富的食品、饲料样本中的Listeria monocytogenes。该试剂盒能检测低至10拷贝的L. monocytogenes DNA并表现出高度特异性。在一次实验中使用2500拷贝的待测DNA未发现与其他病原体有交叉反应性,甚至包括极度相近Listeria亚种 (参见"mericon L. monocytogenes Kit交叉反应研究结果")。

mericon Salmonella spp Kit

mericon Salmonella spp Kit用于特异性检测来源丰富的食品、饲料样本中的几种Salmonella亚种。该试剂盒能检测低至10拷贝的Salmonella DNA并对该种属病原体表现出高度特异性。在一次实验中使用2500拷贝的待测DNA未发现与其他病原体有交叉反应性(参见"mericon Salmonella spp Kit交叉反应研究结果")。

mericon Shigella spp Kit

mericon Shigella spp Kit用于特异性检测来源丰富的食品、饲料样本中的几种Shigella亚种。该试剂盒能检测低至10拷贝的Shigella DNA并对该种属病原体表现出高度特异性。在一次实验中使用2500拷贝的待测DNA未发现与其他病原体有交叉反应性(参见"mericon Shigella spp Kit交叉反应研究结果")。

mericon S. aureus Kit

mericon S. aureus Kit用于特异性检测来源丰富的食品、饲料样本中的Staphylococcus aureus。该试剂盒能检测低至10拷贝的S. aureus DNA并表现出高度特异性。在一次实验中使用2500拷贝的待测DNA未发现与其他病原体有交叉反应性(参见"mericon S. aureus Kit交叉反应研究结果")。

mericon VTEC stx1/2 Kit

Tmericon VTEC stx1/2 Kit用于特异性检测来源丰富的食品、饲料样本中的Escherichia coli菌属,它能产生verotoxins也称为Shiga毒素。该试剂盒能检测低至10拷贝的产生志贺样毒素的E. coli DNA并表现出高度特异性。在一次实验中使用2500拷贝的待测DNA未发现与其他病原体有交叉反应性(参见"mericon VTEC stx1/2 Kit交叉反应研究结果")。

mericon Y. enterocolitica Kit

mericonY. enterocolitica Kit用于特异性检测来源丰富的食品、饲料样本中的Yersinia enterocolitica并能特异性检测人病原体血清型0:3、0:5、0:6、0:8、0:9 和0:5:27。该试剂盒能检测低至10拷贝的Yersinia enterocolitica DNA并表现出高度特异性。在一次实验中使用2500拷贝的待测DNA未发现与其他病原体有交叉反应性(参见"mericon Y. enterocolitica Kit交叉反应研究结果")。

mericon Campylobacter spp Kit交叉反应研究结果
病原体 结果 病原体 结果
Campylobacter jejuni + Campylobacter coli +
Campylobacter laridis + Campylobacter upsaliensis +
Salmonella spp. VTEC stx1
Clostridium perfringens VTEC stx2
Legionella erythra Yersinia enterocolitica
Staphylococcus aureus Listeria monocytogenes
mericon Campylobacter triple Kit交叉反应研究结果
病原体 结果 病原体 结果
Campylobacter jejuni + Campylobacter coli +
Campylobacter laridis + Campylobacter upsaliensis
Salmonella spp. VTEC stx1
Clostridium perfringens VTEC stx2
Legionella pneumophila Yersinia enterocolitica
Staphylococcus aureus Listeria monocytogenes
Escherichia coli Bacillus cereus
mericon Cronobacter spp Kit交叉反应研究结果
病原体 结果 病原体 结果
Cronobacter sakazakii + Listeria monocytogenes
Staphylococcus aureus Escherichia coli
Salmonella spp. Legionella erythra
Clostridium perfringens Bacillus cereus
Legionella pneumophila Yersinia enterocolitica
Shigella flexneri VTEC stx1
Campylobacter jejuni VTEC stx2
mericon Legionella spp Kit交叉反应研究结果
病原体 结果 病原体 结果
Legionella pneumophila + Legionella erythra +
Salmonella thyphimurium Escherichia coli
Shigella flexneri Bacillus cereus
Clostridium perfringens Yersinia enterocolitica
Campylobacter jejuni
mericon L. pneumophila Kit交叉反应研究结果
病原体 结果 病原体 结果
Legionella pneumophila + Legionella erythra
Salmonella thyphimurium Escherichia coli
Shigella flexneri Bacillus cereus
Clostridium perfringens Yersinia enterocolitica
Campylobacter jejuni
mericon Listeria spp Kit交叉反应研究结果
病原体 结果 病原体 结果
Listeria monocytogenes + Cronobacter sakazakii
Listeria welshimeri + Legionella pneumophila
Listeria seeligeri + Staphylococcus aureus
Listeria ivanovii + Bacillus cereus
Listeria innocua + Yersinia enterocolitica
Listeria marthii + VTEC
Listeria grayi + Legionella erythra
Listeria rocoutiae + Shigella flexneri
Campylobacter jejuni Clostridium perfringens
Escherichia coli
mericon L. monocytogenes Kit交叉反应研究结果
病原体 结果 病原体 结果
Listeria monocytogenes + Cronobacter sakazakii
Clostridium perfringens Bacillus cereus
Staphylococcus aureus Legionella erythra
Salmonella spp. Campylobacter jejuni
Yersinia enterocolitica Legionella pneumophila
Shigella flexneri VTEC stx1
Escherichia coli VTEC stx2
Listeria innocua Listeria welshimeri
Listeria ivanovii spp. Ivanovii Listeria grayi
Listeria seeligeri
mericon Salmonella spp Kit交叉反应研究结果
病原体 结果 病原体 结果
Salmonella spp + Cronobacter sakazakii
Clostridium perfringens Bacillus cereus
Staphylococcus aureus Legionella erythra
Listeria monocytogenes Campylobacter jejuni
Yersinia enterocolitica Legionella pneumophila
Shigella flexneri VTEC stx1
Escherichia coli VTEC stx2
mericon Shigella spp Kit交叉反应研究结果
病原体 结果 病原体 结果
Shigella spp. + Listeria monocytogenes
Staphylococcus aureus Escherichia coli
Salmonella spp. Bacillus cereus
Clostridium perfringens Yersinia enterocolitica
Campylobacter jejuni Legionella erythra
VTEC stx1 VTEC stx2
mericon S. aureus Kit交叉反应研究结果
病原体 结果 病原体 结果
Staphylococcus aureus + Listeria monocytogenes
Cronobacter sakazakii Escherichia coli
Salmonella spp. Legionella erythra
Clostridium perfringens Bacillus cereus
Legionella pneumophila Yersinia enterocolitica
Shigella flexneri VTEC stx1
Campylobacter jejuni VTEC stx2
mericon VTEC stx1/2 Kit交叉反应研究结果
病原体 结果 病原体 结果
VTEC stx1 + VTEC stx2 +
Campylobacter jejuni Escherichia coli
Salmonella spp. Legionella erythra
Clostridium perfringens Bacillus cereus
Legionella pneumophila Yersinia enterocolitica
Staphylococcus aureus Listeria monocytogenes
mericon Y. enterocolitica Kit交叉反应研究结果
病原体 结果 病原体 结果
Yersinia enterocolitica + Listeria monocytogenes
non-pathogenic Yersinia
enterocolitica
Escherichia coli
Staphylococcus aureus Bacillus cereus
Clostridium perfringens Salmonella spp.
Campylobacter jejuni Legionella erythra
VTEC stx1 VTEC stx2
Principle

利用real-time PCR进行病原体检测是基于病原体基因组特定区域的扩增。在real-time PCR及RT-PCR仪上,用荧光染色检测扩增的基因组。这些通常与特异性结合扩增产物的寡核苷酸探针连接。随着扩增产物的积累,探针上发出的荧光信号强度增加。在PCR运行的过程中通过监测荧光强度来检测扩增的基因组而无需随后重新打开反应管。

mericon PCR Assays含有一个内参以检测可能存在的PCR抑制,有助于直接得出结果(参见"可能的PCR结果总结")。

每对PCR引物探针都高度特异且靶向作用于待测病原体基因组特定保守区,已被生物信息学及实验所验证。

可能的PCR结果总结
扩增的内参 扩增的被测DNA 意义
+ + 样品对靶DNA呈阳性
+ 样品对靶DNA呈阴性
抑制导致PCR失败
Procedure

使用专门的样品制备试剂盒从来源丰富的食品、饲料样本中提取DNA。用mericon Bacteria Kit从革兰氏阴性菌中分离细菌DNA,用mericon Bacteria Plus Kit从革兰氏阳性菌中分离细菌DNA。

分离出的纯DNA在QIAGEN的Rotor-Gene Q实时荧光定量PCR分析仪上进行real-time PCR分析,73分钟出结果。此实验方案被证实也可用于其他板式热循环仪。

结果用简明的PCR曲线表示。

Applications

mericon Pathogen Detection Assays用于对食品、饲料及药品中特定的细菌病原体进行real-time PCR检测。

Feature
Specifications
Quantitative/qualitative Qualitative (Quantitative for Quant L pneumophilia and Quant Legionella spp)
Recommended sample prep mericon DNA Bacteria Kit (Gram-negative bacteria) or mericon DNA Bacteria Plus Kit (Gram-positive bacteria) or QIAamp UCP Pathogen Mini Kit (Legionella-specific)
RUO/CE/ASR RUO
Sample type Bacterial preculture from food and water samples after concentration, e.g. filtration (for Legionella- specific Kits)
Thermal cycler Rotor-Gene Q and other cyclers
What detected DNA of respective pathogen

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Template Files
2
Rotor-Gene Q template files for starting and analyzing PCR runs
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Rotor-Gene Q template files for starting and analyzing PCR runs
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