Investigator ESSplex SE Plus Kit

同时扩增新欧洲标准位点、外加SE33和性别鉴定基因Amelogenin

  • 90分钟左右即可完成PCR反应
  • 高灵敏度和抑制剂抗性
  • 信噪比高,检验结果质量高
  • 即用型快速反应预混液,实验方案便捷
  • 同时扩增17个位点
Investigator ESSplex SE Plus Kit可扩增新欧洲标准系列(ESS)中的15个STR位点,外加SE33和性别鉴定基因Amelogenin,符合由法庭科学中心欧洲协作组织网站(ENFSI)推荐的标记物,并得到欧盟协会认证。该试剂盒采用QIAGEN快速PCR技术,90分钟即可完成扩增。使用优化的染料BT5 Matrix进行分析校准。 痕量DNA即可获得灵敏度高、快速和可靠的结果,Investigator ESSplex SE Plus Kit适用于多种法医学应用和亲子鉴定。
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Product Cat. no. List price:
Investigator ESSplex SE Plus Kit (100)
Primer mix, Fast Reaction Mix including HotStarTaq Plus DNA Polymerase, Control DNA, allelic ladder ESSplex SE Plus, DNA size standard 550 (BTO), and nuclease-free water
381545
Inquire
Investigator ESSplex SE Plus Kit (400)
Primer mix, Fast Reaction Mix including HotStarTaq Plus DNA Polymerase, Control DNA, allelic ladder ESSplex SE Plus, DNA size standard 550 (BTO), and nuclease-free water
381547
Inquire

The Investigator ESSplex Plus Kit is intended for molecular biology applications in forensic, human identity, and paternity testing. This product is not intended for diagnosis, prevention, or treatment of a disease. This product is not available in all countries.


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Performance

Investigator ESSplex SE Plus Kit使用快速循环技术,90分钟左右即可获得可靠的结果。

新型的快速反应混合液基于经验证的QIAGEN多重PCR技术,但经过进一步优化提高了对抑制剂的耐受性。性能测试表明,提高6种抑制剂(腐植酸、血红素、单宁酸、靛红、钙和胶原)的浓度,也有较高的灵敏度和较好的抑制剂抗性。因此,能够获得可靠、高质量的结果。

使用GeneAmp 9700热循环仪(标准模式)和Applied Biosystems 3130 Genetic Analyzers对Investigator ESSplex SE Plus Kit进行验证和评估。

在标准条件下,DNA的推荐使用量为0.5 ng。内部验证显示使用0.2–2 ng DNA获得稳定平衡的结果,使用<0.1 ng DNA获得可靠的结果。

Principle

Investigator ESSplex SE Plus Kit使用QIAGEN快速循环PCR技术确保快速同时扩增15个STR位点,包括新欧洲标准系列(ESS),外加SE33和性别鉴定基因Amelogenin。其中含有精心设计的引物,避免等位基因重叠,从而减少误译的可能性。

Procedure

可同时在单独的PCR中扩增欧洲标准系列的16个多态性STR位点和性别鉴定基因Amelogenin,并用染料标记,染料参见下表“Investigator ESSplex Kit染料和相应的标记物”。

将扩增得到的位点进行毛细管电泳。使用专业软件,如QIAGEN's Investigator IDproof或IDproof Mixture Software对结果进行分析。参见下表“技术规格”以获得更多信息。

Investigator ESSplex SE Plus Kit染料和相应的标记物
染料标记物
6-FAM Amelogenin TH01 D3S1358 vWA D21S11
BTG D16S539 D1S1656 D19S433 SE33
BTY D10S1248 D22S1045 D12S391 D8S1179 D2S1338
BTR D2S441 D18S51 FGA
技术规格
每次扩增反应的体积 25 μl
基质 BT5
荧光标签 6-FAM、BTG、BTY、BTR和BTO
基因分析仪 ABI PRISM 310、ABI PRISM 3100/3100-Avant、Applied Biosystems 3130/3130xl或Applied Biosystems 3500/3500xL Genetic Analyzers
分析软件 QIAGEN Investigator IDproof或Investigator IDproof Mixture Software、Applied Biosystems GeneMapper ID、GeneMapper ID-X或Genotyper Software
Applications

Investigator ESSplex SE Plus Kit用于从血液、口腔拭子和法医痕迹中快速、可靠的分析DNA图谱,高度适用于: 

罪犯数据库和法医检材样本
亲子鉴定 
种群基因研究 
其他人类身份鉴定

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