用于优化的一步转染siRNA

  • 加入到细胞中孵育即可,节省时间
  • 高效转染,减少手动操作
  • 多次实验均可获得可靠结果

FlexiTube siRNA Premix是siRNA和转染试剂的混合物,通过预混siRNA和试剂,省去了混合及复合物形成所需的时间。此外,应用FlexiTube siRNA Premix无需计算siRNA与试剂的最佳比值。目前提供适用于人类和小鼠基因的FlexiTube siRNA Premix。

Search in GeneGlobe
You can search for the following terms:
  • Entrez Gene IDs (e.g., 835)
  • RefSeq IDs (e.g., NM_032983, NP_116765)
  • Gene symbols (e.g., CASP2)
  • Cat. no. (e.g., SI00299551, QT01342509)
  • Sanger ID or Accession (e.g., hsa-let-7b, MI0000063)
  • CpG loci identification numbers (CG#) (e.g., CG17753661)

Do not enter species information in the Search box. Use the drop-down list to select your species of interest.
When searching for miRNAs, do not omit the hyphens. Use hyphens or spaces (e.g., search for hsa-let-7b or hsa let 7b. Do not search for hsalet7b).

Advanced search settings



Species:
Products
Product Product no. Cat. no. List price:
 
 
Show details
    varies
Can't order online?
To place an order via phone, email or for requesting a quote, please provide the product’s name, number and catalog number.
Product Product no. Cat. no. List price:
FlexiTube siRNA Premix
Optimized siRNA–reagent mix; 0.75 nmol siRNA; provided in tubes
1027420 Varies
Inquire
Enter your targets in search box above.
Positive Controls
Negative Controls
Reporter Controls
Configure
Product Product no. Cat. no. List price:
 
 
Show details
    varies
Can't order online?
To place an order via phone, email or for requesting a quote, please provide the product’s name, number and catalog number.

Can’t order online or need plate formats? Order Forms and Plate Orders

The FlexiTube siRNA Premix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
  • Main Image Navi
Rapid, efficient knockdown.|Phenotype analysis after knockdown.|
HepG2 cells were transfected with various amounts of FlexiTube siRNA Premix targeted against lamin A/C. All resulted in highly efficient lamin A/C knockdown with no need for optimization.|HeLa, MCF-7, and HepG2 cells were transfected with FlexiTube siRNA Premix containing AllStars Negative Control siRNA (Neg. control) or FlexiTube siRNA Premix containing AllStars Hs Cell Death Control siRNA (Cell death). After 72 hours, cells were examined by light microscopy. The cell death phenotype caused by AllStars Hs Cell Death Control siRNA was clearly visible.|
Performance

FlexiTube siRNA Premix中合理的试剂与siRNA比值,可提供高效的转染和基因抑制(参见"Rapid, efficient knockdown"和"Phenotype analysis after knockdown")。

FlexiTube siRNA Premix含有使用高度创新的HP OnGuard siRNA Design设计的FlexiTube siRNA,具有高效特异的优点。根据来自RNAi试验的数据建立极大容量的数据库,并在此基础上利用神经网络技术进行siRNAs设计,然后使用先进的、非冗余序列数据库和同源分析工具,检查该序列与同一基因组内其他序列的同源性。

性能保证

FlexiTube siRNA承诺提供一次性更换。如果订购了同一靶基因的多个FlexiTube siRNA Premixes,且至少2条未表现≥70%的基因抑制,QIAGEN将提供2条免费的额外siRNA,仅限一次。用户需要提供支持数据,以证明在适宜的转染条件下,siRNA未能成功地在mRNA水平抑制≥70%的靶基因。支持数据需要包括转染率数据,定量沉默数据以及≥70%的阳性对照抑制数据。该承诺自发货之日起6个月内有效。

Principle
FlexiTube siRNA Premix的单个试管中预混有相关基因的siRNA和高效的转染试剂。该试剂盒同时含有可增强siRNA-试剂复合物稳定性的特殊缓冲液。siRNA-试剂的比值经过优化,可提供较高的转染率和基因抑制率。
Procedure

FlexiTube siRNA Premix可即时用于细胞转染,加入到细胞中孵育即可。 常规转染实验中siRNA被稀释后与转染试剂混合,然后孵育混合物以形成siRNA-试剂复合物,使用该试剂盒则只需将复合物加入到细胞中进行孵育,从而显著节省时间和人力。

使用FlexiTube siRNA Premix,无需优化siRNA-试剂比,可以更快的开展RNAi实验。该试剂盒提供siRNA和试剂的最佳比例预混物,最大程度减少或省去包括多重转染的冗长优化实验。

单个FlexiTube siRNA Premix可进行多重转染,降低变异性,确保了实验的一致性并使结果更加可靠。

Applications

FlexiTube siRNA Premix是对少量靶基因进行功能基因组研究和通路分析的理想工具。

Feature
Specifications
Design HP OnGuard design
Format Tube
Guarantee/validation for validated siRNAs
Modification No
Number of transfections 50 standard transfections in 24-well format
Scale or yield 0.75 nmol
siRNA per target gene variable
Species Human, mouse
Target sequence provided Yes

You are not authorized to download the resource