自动纯化高品质总RNA

  • 纯化多达30 μg的总RNA
  • 可靠地处理多达10 mg的标准组织样本
  • 无RNase污染,结果可靠
  • 洗脱体积可调
  • 程序设置简单,插卡即用

EZ1 RNA Cell Tissue Kit利用磁珠技术可从多达10 mg的标准组织样本中自动纯化总RNA,试剂盒包含所有需要的试剂和耗材。纯化试剂预先封填在试剂条中,以确保可快速、便利的加载至仪器中。EZ1 RNA Tissue Mini Kit可用在EZ1 Advanced XL、EZ1 Advanced或BioRobot EZ1工作站上。

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EZ1 RNA Tissue Mini Kit (48)
For 48 preps: Reagent Cartridges (Tissue RNA), Disposable Filter-Tips, Disposable Tip-Holders, Sample Tubes (2 ml), Elution Tubes (1.5 ml), Buffer RLT, RNase-free DNase I, RNase-free water
959034
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The EZ1 RNA Tissue Mini Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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Efficient purification and reproducible yields.|Consistent highly pure RNA.|
Using the EZ1 RNA Tissue Mini procedure, total RNA was purified from 10 mg samples of rat kidney and liver and from 5 mg samples of rat pancreas, spleen, and lung. RNA was purified from 3 replicate samples of each tissue type. Yields of total RNA were determined by absorbance (A260) with correction for background (A320). CV kidney = 15%; CV liver = 1%; CV pancreas = 8%; CV spleen = 10%; CV lung = 22%.|RNA was purified from 2-3 replicate tissue samples from each of 4 types of rat tissue using the EZ1 RNA Tissue Mini procedure. Purified total RNA was examined by scanning spectrophotometry. [A] Kidney, 10 mg; [B] liver, 10 mg; [C] pancreas, 5 mg; [D] spleen, 5 mg.|
Performance

使用EZ1 RNA Tissue Mini Kit纯化获得高产量、高品质总RNA的稳定性好、重复性高(参见"Efficient purification and reproducible yields"和"Consistent highly pure RNA")。

Principle

使用EZ1 RNA Tissue Mini Kit进行自动纯化需使用EZ1 Advanced XL和EZ1 Advanced XL RNA Card,EZ1 Advanced和EZ1 Advanced RNA Card或BioRobot EZ1和EZ1 RNA Card。EZ1卡预装有纯化程序,在控制面板提供操作向导,并提供工作站的运行指令。

仪器使用硅胶膜磁珠技术,可在一次操作中对1–14个样本(EZ1 Advanced XL)或1–6个样本(EZ1 Advanced和BioRobot EZ1)进行纯化。预封装的试剂条和仪器的自动感控锁可降低污染的风险,避免RNases导致的核酸降解。自动纯化流程重复性好、产量线性契合度高,覆盖宽范围的起始样本量。该试剂盒可从各种不同的组织样本类型中纯化获得高品质的总RNA,适用于多种基因表达分析的应用。RNA的产量依赖于多种因素,包括:样本量、组织类型和纯化状态。

Procedure

组织首先放入Buffer RLT中裂解、匀浆。全自动EZ1 RNA纯化操作后,RNA被一步纯化。即使很少量的DNA也会干扰RNA的下游应用,所以采用完整的DNase 裂解步骤去除基因组DNA的污染。总RNA洗脱体积为200 µl、100 µl或50 µl,可直接用于各种下游应用。

Applications

EZ1 RNA Tissue Mini Kit纯化得到的高品质RNA适用于各种下游应用,如:

  • 应用real-time RT-PCR技术进行基因表达分析
  • 应用芯片技术进行基因表达分析
  • 肿瘤学和病理学研究
Feature
Specifications
Applications PCR, real-time RT-PCR, microarray
Elution volume 50–200 µl
For automated processing BioRobot EZ1
Format Sample tube
Main sample type Tissue samples
Processing Automated
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein RNA
Sample amount 5–10 µg
Stabilization Yes
Technology Magnetic particles
Time per run or per prep 40 minutes
Yield <30 µg

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Brochures & Guides
2
Kit Handbooks
1
EZ1 RNA Cell Mini Kit
For purification of total RNA from human and animal cells using EZ1instruments
EZ1 RNA Tissue Mini Kit
For purification of total RNA from easy-to-lyse human and animal tissues using EZ1 instruments
EZ1 RNA Universal Tissue Kit
For purification of total RNA from any type of human and animal tissue using EZ1 instruments
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