每批可从1–6个样本或1–14个样本中自动纯化高品质DNA

  • 快速纯化得到高品质DNA
  • 标准化处理多达40 mg的组织样本
  • 灵活纯化各种类型的组织样本
  • 程序设置简单,插卡即用

EZ1 DNA Tissue Kit使用磁珠技术从多达40 mg的组织样本中自动纯化DNA,含有所有所需试剂和耗材。该试剂盒可配合EZ1 Advanced XL(每批处理1–14个样本)、EZ1 Advanced(每批处理1–6个样本)和BioRobot EZ1(每批处理1–6个样本)工作站使用。预封装试剂条可快速、便捷的加载到工作站中。如需使用EZ1 DNA Tissue Kit从各种类型的组织样本中自动纯化基因组DNA的方案,请参照操作手册。样本类型包括:口腔细胞、培养细胞、干血和石蜡包埋组织切片。

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EZ1 DNA Tissue Kit (48)
For 48 preps: Reagent Cartridges (Tissue), Disposable Filter-Tips, Disposable Tip-Holders, Sample Tubes (2 ml), Elution Tubes (1.5 ml), Buffer G2, Proteinase K
953034
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The EZ1 DNA Tissue Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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Consistent PCR results.|
DNA was purified from various tissues using the EZ1 DNA Tissue procedure. [A] PCR of the bovine obesity single-copy gene (850 bp fragment) using EZ1 purified DNA from bovine tissues. [B] PCR of GAPDH (266 bp fragment) using EZ1 purified DNA from 6 mouse tails. A 2.5 µl aliquot (5%) of each PCR was visualized by agarose gel electrophoresis. M: 100 bp marker; -C: negative control.|
Performance
使用EZ1 DNA Tissue Kit纯化的DNA在PCR等下游应用中获得高重复性结果(参见"Consistent PCR results")。
Principle

使用EZ1 DNA Tissue Kit进行自动纯化需使用EZ1 Advanced XL和EZ1 Advanced XL DNA Tissue Card、EZ1 Advanced和EZ1 Advanced DNA Tissue Card或BioRobot EZ1和EZ1 DNA Tissue Card。EZ1卡预装有纯化程序,在控制面板提供操作向导,并提供工作站的运行指令。使用硅胶膜磁珠技术,一次操作可纯化1–14个样本(EZ1 Advanced XL)或1–6个样本(EZ1 Advanced和BioRobot EZ1)。预封装的试剂条和仪器的自动感控锁可降低污染的风险。全自动流程重现性好,在很广的起始样本量范围内可获得很好的线性结果。

Procedure

首先将组织放入含有蛋白酶K的Buffer G2中进行裂解,然后进行匀浆。全自动EZ1 DNA纯化操作后,DNA被一步纯化。基因组DNA洗脱体积为200 µl、100 µl或50 µl,可直接用于各种下游应用,如PCR。

Applications

使用EZ1 DNA Tissue Kit获得的高品质DNA可用于各种下游应用,包括:

  • 药物基因组研究
  • 肿瘤研究
  • 遗传鉴定检测和法医学
  • STR、VNTR、RAPD、NASBA、SNP和AFLP技术
Feature
Specifications
Applications PCR, real-time RT-PCR, genotyping
Elution volume 200 µl
For automated processing BioRobot EZ1
Format Sample tube
Main sample type Tissue samples
Processing Automated
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein DNA
Sample amount 10–40 mg
Technology Magnetic particles
Time per run or per prep 40 minutes
Yield 9–28 µg

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Brochures & Guides
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Fully automated low- to medium-throughput purification of nucleic acids
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Kit Handbooks
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EZ1 DNA Tissue Kit
For automated purification of DNA from tissue and other samples using EZ1 instruments
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