每次运行可从1–6个样本或1–14个样本中自动纯化高品质DNA

  • 从全血样本中纯化多达9.5 µg的DNA
  • 从白膜层样本中纯化多达14 µg的DNA
  • 灵活的洗脱体积
  • 程序设置简单,插卡即用

EZ1 DNA Blood 350 μl Kit采用磁珠技术从多达200 μl的血液或白膜层样本中快速、自动纯化DNA,含有所有所需试剂和耗材。该试剂盒可配合EZ1 Advanced XL(每批处理1–14个样本)、EZ1 Advanced(每批处理1–6个样本)和BioRobot EZ1(每批处理1–6个样本)工作站使用。预封装试剂条可快速、便捷的加载到工作站中。

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EZ1 DNA Blood 350 µl Kit (48)
For 48 preps: Reagent Cartridge (Blood 350 µl), Disposable Filter-Tips, Disposable Tip-Holders, Sample Tubes (2 ml), Elution Tubes (1.5 ml)
951054
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The EZ1 DNA Blood 350 µl Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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Reproducible yields of high-quality DNA from whole blood.|High-quality DNA from whole blood.|
Genomic DNA was purified from 48 x 350 µl samples (white-cell count 4.9 x106 cells/ml) of human whole blood using the EZ1 DNA Blood 350 µl Kit. Blood samples were taken from 1 individual. Average yields from each run of 6 samples are shown. DNA yield was quantified by absorbance measurements (A260) with background correction. Purified DNA was eluted in 200 µl RNase-free water. Average yield was 8.2 µg (S.D. = 0.23). Average DNA purity (A260/A280) was 1.85 (S.D. = 0.01).|DNA was purified from 200 µl blood samples using the EZ1 DNA Blood 200 µl Kit (upper lanes) and from 350 µl blood samples using the EZ1 DNA Blood 350 µl Kit (lower lanes). Lanes 1–6 and 7–12 are from the first and last of 8 processing runs on the workstation, respectively. M: 1000 bp DNA ladder (100 ng); +: positive control; : negative control. 2 µl (1%) of each eluate was visualized on the agarose gel.|
Performance
全自动流程重现性好(参见"Reproducible yields of high-quality DNA from whole blood"和"High-quality DNA from whole blood"),在很广的起始样本量范围内都能获得很好的线性结果。
Principle

使用EZ1 DNA Blood 350 µl Kit进行自动纯化需使用EZ1 Advanced XL和EZ1 Advanced XL DNA Blood Card或EZ1 Advanced XL DNA Buffy Coat Card、EZ1 Advanced和EZ1 Advanced DNA Blood Card或EZ1 Advanced DNA Buffy Coat Card、BioRobot EZ1和EZ1 DNA Blood Card或EZ1 DNA Buffy Coat Card。EZ1卡预装有纯化程序,在控制面板提供操作向导,并提供工作站的运行指令。使用硅胶膜磁珠技术,每批可纯化1–14个样本(EZ1 Advanced XL)或1–6个样本(EZ1 Advanced和BioRobot EZ1)。预封装的试剂条和仪器的自动感控锁可降低污染的风险。

Procedure

将全血样本(350 µl)加载到工作站中,在全自动EZ1 DNA纯化操作后,DNA被一步纯化。基因组DNA洗脱体积为200 µl、100 µl或50 µl,可直接用于各种下游应用。

Applications

使用EZ1 DNA Blood 350 μl Kit纯化的高品质DNA适用于多种下游应用,包括:

  • 群体遗传学和药物基因组学研究
  • PCR和real-time PCR
  • 基因分型
  • 测序
  • 肿瘤研究
  • SNP、STR、VNTR、RAPD、NASBA和AFLP技术
Feature
Specifications
Applications PCR, real-time RT-PCR, genotyping
Elution volume 200 µl
For automated processing BioRobot EZ1
Format Sample tube
Main sample type Whole blood
Processing Automated
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein DNA
Sample amount 350 µl
Technology Magnetic particles
Time per run or per prep 15–20 minutes
Yield 4.5–9.5 µg

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Brochures & Guides
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Fully automated low- to medium-throughput purification of nucleic acids
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Kit Handbooks
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EZ1 DNA Blood 200 μl Kit
EZ1 DNA Blood 350 μl Kit
For automated purification of DNA from blood and buffy coat using EZ1 instruments
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