从1–14个人类样本或法医检材样本中自动纯化核酸
  • 从多种样本中纯化高品质基因组DNA和RNA
  • 从各种生物医学研究样本中纯化得到病毒RNA/DNA和细菌DNA
  • 每次可处理至多14个样本
  • 全过程数据记录,数据管理简单方便
  • 紫外消毒,防止交叉感染
EZ1 Advanced XL可从多种人类身份认定和法医检材样本、生物医学研究和基因表达分析的样本中纯化核酸。
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EZ1 Advanced XL, System
Robotic workstation for automated purification of nucleic acids from up to 14 samples using EZ1 Kits: includes installation, training, 1-year warranty on parts and labor
9001874
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EZ1 Advanced XL, Priority Package
Robotic workstation for automated purification of nucleic acids from up to 14 samples using EZ1 Kits: includes Priority Package with software, installation, training, 2-year warranty on parts and labor, and 2 preventive maintenance visits
9001875
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EZ1 Advanced XL, Priority Package Plus
Robotic workstation for automated purification of nucleic acids from up to 14 samples using EZ1 Kits: includes Priority Package with software, installation, training, 3-year warranty on parts and labor, and 3 preventive maintenance visits
9001876
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Silicone Grease, BR M48 & BR EZ1
Silicone Grease, BR M48 & BR EZ1
9239334
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EZ1 Advanced XL应与QIAGEN试剂盒配合使用,适用于试剂盒用户手册中指定的应用。


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Simple protocol.|Flexibility and ease of use.|Standardization and traceability.|Safety and reliability.|
1. Insert the EZ1 Advanced XL Card with the desired protocol into the card slot and start the instrument. 2. Place samples and prefilled, sealed reagent cartridges into the instrument. Samples are processed automatically and purified nucleic acids are transferred to elution tubes. 3. Remove pure nucleic acids in 1.5 ml microcentrifuge tubes at a concentration suited to the downstream application.|A wide range of preprogrammed protocols, without the need for any manual data entry, are available on EZ1 Advanced XL Cards for purification of DNA, RNA, or viral nucleic acids. New cards may be purchased — increasing the range of purification protocols without any retraining or costly modifications to the instrument.|The EZ1 Advanced XL instrument sets new standards in data management. Barcode reading enables complete tracking of samples and reagents throughout the entire purification process. For increased process control and reliability, reagent identification and lot numbers are logged together with the expiration date, and a warning is issued if the expiration date has been exceeded. The run report is compatible with subsequent processing by a Laboratory Information Management System (LIMS).|The EZ1 Advanced XL has been designed to ensure high process safety, high performance, and user convenience. A sensor ensures that the instrument door remains closed during sample preparation, reducing contact of laboratory personnel with potentially infectious samples and protecting sample integrity. At the end of each run, an intelligent-design UV lamp decontaminates the inner surface of the instrument. If required, the instrument can be placed in a safety cabinet, providing increased user safety.|
Performance
标准化并可追踪

EZ1 Advanced XL建立数据管理的新标准。阅读条形码可在整个纯化过程中对样本和试剂进行全程追踪。可方便的输入备注和检测信息。试剂认证、批号和有效期同时登记,以提高过程控制和可靠度。如试剂超过有效期,后会发出警告。在个实验方案结束时会自动生成运行报告,包含维护过程和消毒日期等所有仪器信息。每次运行结束之后,仪器会生成一个pdf和一个csv文档。可便利的将数据传送至本地打印机或计算机上,报表可以用LIMS(实验室信息管理系统)处理。

经验证的技术、表现卓越

经验证的EZ1 Kits配合EZ1 Advanced XL Cards可确保表现卓越的纯化过程。EZ1 Advanced XL具有无与伦比的易用性,可快速纯化,并具有高度可重复性,无需特别培训仪器使用,也不要求具备操作经验。该仪器的应用范围可扩增,包括从血液、组织和遗传身份鉴定及法医鉴定样本中提取DNA,从细胞和组织中纯化RNA以及从各种样本类型中提取病毒核酸,可满足需要处理各种样本的实验室对于灵活性的要求。

安全性和可靠性

EZ1 Advanced XL具有独特的设计,可确保纯化过程高度安全、性能卓越并且使用简单。工作站可放置于小柜中,在必要的情况下确保用户安全。此外,配有可确保仪器的盖子在样本制备过程中关闭的传感器 ,减少实验室人员与可能感染的样本的接触,保护样本完整、不被污染。在每次运行结束后,智能的紫外线灯可对工作站表面进行消毒,减少不同批次间的样本污染。紫外线灭菌可高效消除革兰氏阳性菌和阴性菌(参见下表)。

E. coli菌群数量
照射时间(分钟) 照射次数 1 2 3 4 5 6
30 1 0 0 0 0 0 0
30 2 0 0 0 1 0 0
30 3 10 0 0 0 0 3
30 对照 311 352 367 367 351 396
60 1 0 0 0 0 0 0
60 2 0 0 0 0 0 0
60 3 0 0 0 0 0 0
60 对照 348 307 326 326 396 385
使用EZ1 Advanced的紫外灯照射6份同样的Escherichia coli培养液(每份20 μl ),之后在合适的条件下,在培养皿中进行培养。对每份培养皿进行不同次数的紫外灯照射。紫外线能高效杀灭细菌。
S. haemolyticus 菌群数量
照射时间(分钟) 照射次数 1 2 3 4 5 6
30 1 12 10 17 2 6 4
30 2 0 0 0 0 0 0
30 3 10 0 2 11 0 3
30 对照 248 256 396 314 321 267
60 1 0 0 0 0 0 0
60 2 0 0 0 0 0 0
60 3 10 0 0 0 0 0
60 对照 264 305 368 292 347 388
使用EZ1 Advanced的紫外灯照射6份同样的Staphylococcus haemolyticus培养液(每份20 μl ,浓度为15,700 CFU/ml),之后在合适的条件下,在培养皿中进行培养。对每份培养皿进行不同次数的紫外灯照射。紫外线能高效杀灭细菌。
Principle

样本自动化制备可实现高效、标准化的核酸纯化,将手工操作可能引起的污染降至最低,确保用户安全和可靠的样本处理。

EZ1 Advanced XL配合经验证的EZ1 Kits,从各种样本中纯化DNA、RNA和病毒核酸,经纯化的核酸可直接用于下游应用。简单易用的工作站配合优化的实验方案和工作台设置、以及预制的密封试剂条可简化核酸纯化。

Procedure
灵活性、使用方便

EZ1 Advanced XL为用户带来便利的自动化工作站。提供多种适用于纯化DNA、RNA或病毒核酸的实验方案。EZ1 Advanced XL可在20分钟内纯化1–14个样本。该仪器通过键盘操作,VFD(真空荧光显示屏)上会显示仪器信息。为了方便根据不同样本制备的需求进行调整,可将多至4台EZ1 Advanced XL连接到一个外部电脑(QIAGEN提供),将通量提高至56个样本。

所有处理步骤均由仪器自动完成:包括穿刺试剂条,到洗脱高纯度核酸。无需使用另外的计算机,EZ1 Kits包括所有样本制备所需的试剂和附件。EZ1 Kits提供预装的、直至仪器门关闭开始运行都保持密封的试剂条,降低反应体系构建过程中的污染风险。

适用于多种应用的EZ1 Kits和Advanced XL Cards

EZ1 Advanced XL应用经验证的EZ1 Kits并配合预编在EZ1 Advanced XL Cards上的操作步骤。EZ1 Kits提供各种广泛的应用,包括遗传身份鉴定、法医鉴定、生物医学研究和基因表达分析等(参见下表)。

EZ1 Advanced Cards与EZ1 Kits
程序卡 试剂盒 样本
EZ1 Advanced XL DNA Blood Card EZ1 DNA Blood 200 µl  Kit 血液类样本
EZ1 Advanced XL DNA Blood Card EZ1 DNA Blood 350 µl Kit 血液类样本
EZ1 Advanced XL DNA Dried Blood Card EZ1 DNA Tissue Kit 干血
EZ1 Advanced XL DNA Buffy Coat Card EZ1 DNA Blood 350 µl Kit 白膜层
EZ1 Advanced XL DNA Tissue Card EZ1 DNA Tissue Kit 组织
EZ1 Advanced XL DNA Paraffin Section Card EZ1 DNA Tissue Kit 石蜡包埋组织
EZ1 Advanced XL DNA Buccal Swab Card EZ1 DNA Tissue Kit 口腔拭子
EZ1 Advanced XL DNA Investigator Card EZ1 DNA Investigator Kit 法医和人类身份认定样本
EZ1 Advanced XL DNA Bacteria Card EZ1 DNA Tissue Kit 人类样本、拭子、活检和细菌培养液
EZ1 Advanced XL Virus Card v2.0 EZ1 Virus Mini Kit v2.0 血清、血浆、脑脊液、尿液、全血、粪便、运输介质、呼吸道样本和干燥拭子样本中的病毒和细菌核酸
EZ1 Advanced XL RNA Card EZ1 RNA Universal Tissue Kit 各种人类或动物组织
EZ1 Advanced XL DSP DNA Blood Card* EZ1 DSP DNA Blood Kit* 人类全血
EZ1 Advanced XL DSP Virus Card* EZ1 DSP Virus Kit* 血清、血浆、脑脊液、尿液、全血、粪便、运输介质、呼吸道样本和干燥拭子样本中的病毒和细菌核酸
* EZ1 Advanced XL DSP Cards和EZ1 DSP Kits在欧洲、美国和加拿大可用于体外诊断。
Applications

使用EZ1 Advanced XL纯化得到的核酸可即用于多种下游应用,包括:

肿瘤、法医学研究和身份认定  
基因分型
传染病研究,包括细菌基因分型
基因表达分析
Feature
Specifications
Features Touch LCD user interface, IC card with protocols/software, barcode reader, UV lamp
Instrument dimensions 510 x 510 x 570 mm (w x d x h)
Kits designed for this instrument QIAGEN EZ1 Kits
Pipetting range 50-1000 µl
Protocols/main application on this instrument Purification of DNA, mRNA, total RNA, and viral RNA and DNA
Samples per run; throughput 1–14 samples per run
Technical data of the instrument Weight 48 kg, 100–240 V AC, 50–60 Hz
Technology Magnetic-particle technology

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Supplementary Protocols
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Supplementary protocol using the EZ1 Advanced or EZ1 Advanced XL with the EZ1 DNA Investigator Kit
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Supplementary protocol using the Flip Cap Rack and the EZ1 Advanced XL Flip Cap Card.
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User-Developed Protocols
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This protocol has been adapted by the San Diego Police Department (SDPD) Crime Laboratory from the standard QIAGEN pretreatment for casework samples, and is intended for the lysis and extraction of DNA from forensic samples of fired (casings) and unfired (cartridges) ammunition.
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