Blood & Cell Culture DNA Maxi Kit

从血液和培养细胞中纯化500 µg的高分子量DNA

  • 可靠纯化150 kb的DNA
  • 无需苯酚或氯仿抽提
  • 方便同时处理多个样本

Blood & Cell Culture DNA Kit提供重力流阴离子交换柱和缓冲液,从多种生物样本中高效纯化基因组DNA 。纯化得到的DNA片段长达150 kb,片段平均长度为50–100 kb。

Product Product no. Cat. no. List price:
 
 
Show details
    varies
Can't order online?
To place an order via phone, email or for requesting a quote, please provide the product’s name, number and catalog number.
Product Cat. no. List price:
Blood & Cell Culture DNA Maxi Kit (10)
10 QIAGEN Genomic-tip 500/G, QIAGEN Protease, Buffers
13362
Inquire
The Blood & Cell Culture DNA Maxi Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
  • Main Image Navi
QIAGEN Genomic-tip procedure.|Genomic DNA of up to 150 kb.|
Samples are first lysed (tissue samples are mechanically disrupted) and proteins simultaneously denatured in the appropriate lysis buffer. QIAGEN Protease or Proteinase K is then added and after a suitable incubation period, lysates are loaded onto the QIAGEN Genomic-tip. DNA binds to the column while other cell constituents pass through. Following a wash step to remove any remaining contaminants, pure, high-molecular-weight DNA is eluted and precipitated with isopropanol.|Pulse-field gel electrophoresis of DNA (2 µg) purified using QIAGEN Genomic-tips. M: markers.|
Performance

QIAGEN Genomic-tip分离过程非常温和,导致的DNA剪切可以忽略。纯化得到的DNA可长达150 kb,平均长度为50–100 kb(参见"Genomic DNA of up to 150 kb")。纯化得到的DNA不含RNA、蛋白质和代谢物等污染物,A260/A280值为1.7–1.9。

纯化得到的DNA片段长度较长,尤其适用于构建高品质DNA文库以用于不同平台的二代测序,因此受到多家核心机构的推荐。

Blood & Cell Culture DNA Maxi Kit的标准产量
样本来源 产量(µg)
全血(人类)
20 ml 350–400
培养细胞(HeLa)
1 x 108 350–450

Principle

Blood & Cell Culture DNA Kit中的QIAGEN Genomic-tip利用QIAGEN独有的阴离子交换技术,从多种生物样本中纯化高分子量DNA,而无需苯酚或氯仿抽提。裂解缓冲液针对不同类型样本进行优化,使得核酸酶、组蛋白和DNA结合蛋白等蛋白质以及潜在的传染性病毒等快速变性。在该缓冲液的pH值以及低盐环境中,DNA与QIAGEN交换柱上的树脂结合,蛋白质、碳水化合物和代谢物等细胞组分会随流出液流出。在高盐的洗脱液中可获得纯化的DNA。Genomic-tip基于重力流技术,无需专人照看。该操作最大程度节约了手动操作时间,是对多个样本进行同时处理的理想选择。

Procedure

首先裂解样本(组织样本需要机械破碎),蛋白质在适宜的裂解缓冲液中变性(参见"QIAGEN Genomic-tip procedure")。然后加入QIAGEN Protease或蛋白酶K,温育后,将裂解产物上样到QIAGEN Genomic-tip。DNA吸附到交换柱上,其他细胞组分随流出液流出。随后进行洗涤,将残留的污染物洗去。最后洗脱得到纯化的高分子量DNA,在异丙醇中沉淀。针对血液和培养细胞的完整流程中手动操作仅需30分钟。

Blood & Cell Culture DNA Kits是即用型试剂盒,含有从全血和培养细胞中纯化高分子量DNA所需的全部组分。

Applications

Blood & Cell Culture DNA Maxi Kit纯化得到的DNA非常适用于以下应用:

  • Sanger测序和二代测序
  • RFLP分析
  • 靶基因分析
  • 转基因动物的筛查
  • DNA图谱研究
  • PCR扩增
Feature
Specifications
Applications PCR, RFLP, blotting, screening
Elution volume 0.1–2 ml
Format Genomic tip
Main sample type Blood, cells
Processing Manual
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein DNA
Sample amount 20 ml/1 x 10e8
Technology Anion-exchange technology
Yield 350–400 µg

You are not authorized to download the resource

References
0