artus Enterovirus LC RT-PCR Kit RUO

使用real-time PCR方法定量检测肠道病毒RNA

  • 使用内参,具有高度可靠性
  • 可灵敏检测3.2拷贝/µl的RNA
  • 提供4个标准品,可进行准确的定量检测
artus Enterovirus LC RT-PCR Kit是使用real-time PCR技术的即用型分子检测试剂盒。该试剂盒包含对肠道病毒RNA进行可靠定量检测的所有所需试剂,并已经过优化。
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artus Enterovirus LC RT-PCR Kit (24) RUO
For 24 reactions on the LightCycler 1.1/1.2/1.5 Instruments: Master, 4 Quantitation Standards, Internal Control, Water (PCR grade)
4510003
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artus Enterovirus LC RT-PCR Kit (96) RUO
For 96 reactions on the LightCycler 1.1/1.2/1.5 Instruments: Master, 4 Quantitation Standards, Internal Control, Water (PCR grade)
4510005
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For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
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Reliable quantitation of enterovirus load.|
Detection of the quantitation standards (Enterovirus LC QS 1–4) on LightCycler 1.1/1.2/1.5 instruments using the artus Enterovirus LC RT-PCR Kit. NTC: no template control.|
Performance

为确保高灵敏度,artus Enterovirus LC RT-PCR Kit已经过优化,可检测到低拷贝数的肠道病毒RNA。该试剂盒在PCR反应中的分析灵敏度为3.2拷贝/µl。

artus Enterovirus LC RT-PCR Kit的性能
试剂盒artus Enterovirus LC RT-PCR Kit
样本类型 脑脊液
分析灵敏度 3.2拷贝/µl
Principle

artus Enterovirus LC RT-PCR Kit的原理是使用real-time PCR技术,扩增并实时检测肠道病毒基因组的一段特定序列。该试剂盒特异性强、灵敏度高,且重复性好。

每个artus Enterovirus LC RT-PCR Kit中都包含4个肠道病毒标准品(参见"Reliable quantitation of enterovirus load")。使用这些标准品可确保病毒的准确定量。此外,该试剂盒还含有异源扩增系统,即内参,可识别PCR抑制剂。内参(IC)可在PCR中通过不同的荧光通道检测,而不会降低肠道病毒RT-PCR的检测灵敏度。

artus Enterovirus LC RT-PCR Kit的规格
试剂盒 artus Enterovirus LC RT-PCR Kit
样本类型 脑脊液
扩增子 肠道病毒基因组的一段长为114 bp的序列
Procedure

artus Enterovirus LC RT-PCR Kit包含对肠道病毒RNA进行可靠定量检测的所有所需试剂,并已经过优化。只需在即用型PCR预混液中加入模板RNA,即可按照试剂盒操作手册中的优化程序,在real-time PCR仪上开始反应。

推荐使用QIAamp Viral RNA Mini Kit进行RNA纯化。

Applications
artus Enterovirus LC RT-PCR Kit可快速、灵敏、准确的定量检测脑脊液中的肠道病毒RNA。该试剂盒可在LightCycler 1.1/1.2/1.5仪器上使用。
Feature
Specifications
Quantitative/qualitative Quantitative
Recommended sample prep QIAamp Viral RNA Mini Kit
RUO/CE/ASR RUO
Sample type Cerebrospinal fluid (CSF)
Thermal cycler LightCycler
What detected Enterovirus RNA

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