AllStars Hs Cell Death Control siRNA

用于在人类细胞中进行siRNA转染条件优化及阳性对照实验

  • 可便利的进行优化实验和阳性对照实验
  • 高通量RNAi研究的对照
  • 使用光学显微镜,即可进行简单的评估
  • 广泛适用于各种细胞类型,效率高

AllStars Hs Cell Death Control siRNA是高效的siRNA混合物,靶向细胞存活所必需的广泛表达的人类基因。这些基因的沉默导致较高程度的细胞死亡,并可由光学显微镜观测到。只需在AllStars Hs Cell Death Control siRNA转染后的48到96小时内,直接在光学显微镜下观测细胞,即可快速估算转染效率,无需任何复杂、繁琐的下游实验。AllStars Hs Cell Death Control siRNA是进行siRNA转染优化的有效工具,也可用作常规的阳性对照。

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The AllStars Hs Cell Death Control siRNA is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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High level of cell death.|Cell death in primary cells.|Easy siRNA transfection optimization.|
[A]  SW620 cells or  [B] SW480 cells (5 x 103 cells per well in 96-well plates) were transfected with 20 nM of AllStars Hs Cell Death Control siRNA or AllStars Negative Control siRNA or an siRNA targeting PLK (a well-known target for induction of cell death). Untransfected cells and cells treated with transfection reagent only were also analyzed. After 72 hours, cell death was quantified using a CellTiter-Blue assay (Promega). AllStars Hs Cell Death Control siRNA resulted in high levels of cell death in both cell types. (Data kindly provided by Dr. Amanda Hummon and Dr. Thomas Reid, National Cancer Institute, Maryland, U.S.A.)|Primary human NHBE cells (2 x 104) were transfected with 10 nM, 20 nM, or 100 nM AllStars Hs Cell Death Control siRNA or 100 nM nonsilencing siRNA (AllStars Negative Control siRNA) using HiPerFect Transfection Reagent. After 48 hours, cell death was observed by light microscopy.|MCF-7 cells (5 x 103) in 96-well plates were transfected with various amounts of AllStars Hs Cell Death Control siRNA or 50 nM nonsilencing siRNA (AllStars Negative Control siRNA) using HiPerFect Transfection Reagent. After 72 hours, cell death was observed by light microscopy. Optimal conditions were 5 nM siRNA and 1.5/2 μl HiPerFect Reagent or 2 nM siRNA and 2 μl HiPerFect Reagent.|
Performance

广泛适用于各种细胞类型的快速检测

AllStars Hs Cell Death Control siRNA适用于多种细胞类型(参见下表)。

  AllStars Hs Cell Death Control siRNA适用的细胞类型
 细胞系  细胞类型
 293  Human embryonic kidney cells
 A549  Human alveolar basal epithelial cells
 CaCo  Human colonic carcinoma cells
 HeLa  Human cervical cancer cells
 HeLa S3  Human cervical cancer cells
 HepG2  Human hepatoma cells
 Huh7  Human hepatoma cells
 MCF-7  Human breast adenocarcinoma cells
 MDA-MB-231  Human breast adenocarcinoma cells
 ME180  Human cervical squamous carcinoma cells
 SW 480  Human colon adenocarcinoma cells
 原代细胞  细胞类型
 HUVEC  Primary human umbilical vein endothelial cells
 NHBE  Primary normal human bronchial epithelial cells
 NHLF  Primary normal human lung fibroblast cells

细胞沉默和死亡水平高

使用AllStars Hs Cell Death Control siRNA得到的细胞沉默率很高;经定量RT-PCR测定,所有目标mRNA的沉默率均高于95%。

AllStars Hs Cell Death Control广泛适用于多种细胞类型,其在细胞死亡方面的表现,明显优于市场上其他的有毒siRNA对照。使用AllStars Hs Cell Death Control可达到稳定的细胞死亡水平,可确保进行高效的对照实验(参见"High Level of Cell Death")。

Principle

便利的进行转染优化

在选择新实验或新细胞系所需的RNAi时,需要在不同条件下进行多次转染实验,以确定最佳转染条件。可使用AllStars Hs Cell Death Control siRNA进行这些优化实验(参见"Easy siRNA transfection optimization"和"Cell death in primary cells")。引起最高细胞死亡水平的转染条件与非沉默siRNA对照的转染条件可用于将来的相关实验。

可靠的高通量RNAi筛选

进行高通量RNAi筛选时,需要使用阳性内参,以确保每个孔板的筛选都是在最佳转染条件下进行的。阳性内参可以通过外形观察直接分析,因此可即时观察转染效率。在每个孔板上都使用AllStars Hs Cell Death Control siRNA,可经济、快速的估算每个孔板的转染率。

Procedure

使用AllStars Hs Cell Death Control siRNA可在转染后的48到96小时内,使用光学显微镜观察细胞,快速估计转染率,无需任何复杂、繁琐的下游实验。

AllStars Hs Cell Death Control siRNA的订购规格包括1 nmol、5 nmol和20 nmol。同时有适用于96孔板(FlexiPlate siRNA)的1 nmol、0.25 nmol和0.1 nmol规格。 

Applications
RNAi实验建立
优化转染方案
高通量RNAi筛选
Feature
Specifications
Design Designed using HP OnGuard siRNA design
Format Tube
Modification Yes
Scale or yield 5 nmol, 20 nmol
Species Human
Target sequence provided No

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