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PyroMark Buffers and Solutions

采用焦磷酸测序技术,优化模板DNA的制备和分析

    使用PyroMark真空工作站可快速、直接的从PCR扩增子生成焦磷酸测序模板,这些缓冲液和溶液专为此而设计。PyroMark Binding Buffer可促进亚硫酸氢盐转化后的PCR扩增子与覆盖链酶亲和素的琼脂糖凝胶微球结合。随后将这些微球固定在工作站的真空仪器上。PyroMark Denaturation Solution可温和的从作为焦磷酸测序模板的生物素标记链上分离其互补链,PyroMark Wash Buffer确保只有生物素标记的单链DNA结合在真空容器中。最后,当单链DNA释放到含焦磷酸测序引物的反应容器时,PyroMark Annealing Buffer可加强引物和模板的结合。

    Cat No./ID: 979006
    PyroMark Binding Buffer (200 ml)
    For binding of biotinylated PCR product to Sepharose beads
    Cat No./ID: 979007
    PyroMark Denaturation Sol. (500 ml)
    For denaturation of double-stranded PCR product into single-stranded DNA template
    Cat No./ID: 979008
    PyroMark Wash Buffer (conc., 200 ml)
    For use with the PyroMark Q24 Vacuum Workstation and PyroMark Q96 Vacuum Workstation to wash and neutralize the immobilized DNA
    Cat No./ID: 979009
    PyroMark Annealing Buffer
    For annealing of sequencing primer to DNA template

    PyroMark Buffers and Solutions适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


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