QIAamp DNA Blood Midi Kit
从血液和相关体液中纯化基因组DNA、线粒体DNA或病毒DNA,最高可达60 μg
- 快速纯化高品质、即用型DNA
- 无需有机提取或乙醇沉淀
- 重复性好,产量高
- 完全去除污染物和抑制剂
QIAamp DNA Blood Midi Kits应用硅胶模技术,用于实验室从全血、血浆、血清和其它体液中纯化DNA。QIAamp DNA Blood Midi Kit可处理0.3–2 ml新鲜或冷冻的人类全血。QIAamp Midi离心柱可在离心机或真空装置上处理(使用真空管需要额外的缓冲液AW1 [货号:19081]和AW2 [货号:19072] )。
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QIAamp DNA Blood Midi Kit (20)
For 20 DNA midipreps: 20 QIAamp Midi Spin Columns, QIAGEN Protease, Buffers, Collection Tubes (15 ml)
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51183
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QIAamp DNA Blood Midi Kit (100)
For 100 DNA midipreps: 100 QIAamp Midi Spin Columns, QIAGEN Protease, Buffers, Collection Tubes (15 ml)
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51185
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The QIAamp DNA Blood Midi Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
QIAamp Spin Column procedure.|Apoptotic banding in stored blood.|Long-range PCR.|Paternity testing by RFLP analysis.|
Optimized buffers lyse samples, stabilize nucleic acids, and enhance selective DNA adsorption to the QIAamp membrane. Alcohol is added and lysates loaded onto the QIAamp spin column. Wash buffers are used to remove impurities and pure, ready-to-use DNA is then eluted in water or low-salt buffer. |Genomic DNA from 8 blood samples stored at 4°C for 1 week. DNA was purified using the QIAamp DNA Blood Mini Kit. When blood is stored at 4°C, the DNA is rapidly degraded due to apoptosis; the resulting apoptotic banding pattern can clearly be seen in these samples. M1: lambda-HindIII; M2:100 bp ladder.|Amplification of a 10 kb fragment of the human ALDH1 gene from genomic DNA isolated from blood. DNA was purified using conventional methods (Phenol) or the QIAamp DNA Blood Maxi Kit (QIAamp). PCR products were sequenced directly. M: 1 kb ladder.|Three paternity cases tested with DNA purified from blood samples with the QIAamp DNA Blood Mini Kit. 1: mother; 2: child; 3: alleged father; 4: child + alleged father. M: markers. Each lane had 3 µg of DNA loaded. Outcome: A: alleged father excluded; B & C: alleged father confirmed. (Data kindly provided by J. James, Gene Proof Technologies, Nashville, TN, USA.)|
Principle
无需苯酚-氯仿抽提。核酸特异性结合到QIAamp硅胶膜上,污染物流走。PCR抑制剂,如:二价阳离子和蛋白,可通过两步有效的洗涤被完全去除,结合在离心柱上的纯核酸可用水或试剂盒中的缓冲液洗脱。QIAamp DNA Blood技术从全血或相关体液纯化得到的基因组、线粒体或病毒DNA,可即用于PCR和印迹实验中。
Procedure
QIAamp DNA Blood Midi Kit采用快速离心柱操作简化了从全血或相关体液中分离DNA的流程(参见"procedure")。该试剂盒可处理带有EDTA、柠檬酸盐和肝素等常规抗凝剂的新鲜或冷冻的全血样本。QIAamp DNA Blood Midi Kit可处理0.3–2 ml的样本,制备时间55分钟。可从1 ml健康全血中获得20–60 µg DNA,洗脱体积100–400 µl。
QIAamp DNA Blood Kits提供全面的操作手册,包括从不同来源的样本中纯化DNA的操作流程。QIAamp样本制备技术已完全经过验证。
Applications
QIAamp DNA Blood Midi Kit使用经验证的QIAamp技术,采用带有15 ml收集管的Midi离心柱从不同来源的样本中纯化DNA。样本来源包括:
新鲜和冷冻的全血或白膜层
血浆或血清
骨髓
淋巴细胞
体液
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Feature
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Specifications
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Applications
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PCR, southern blotting
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Elution volume
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100–400 µl
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Format
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Spin column
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Main sample type
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Whole blood, body fluids
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Processing
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Manual (centrifugation or vacuum)
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Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein
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Genomic DNA, mitochondrial DNA, viral DNA
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Sample amount
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0.3–2 ml
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Technology
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Silica technology
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Time per run or per prep
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55 minutes
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Yield
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20–60 µg
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For large-scale genomic and viral DNA purification from whole blood, plasma, serum, body fluids, lymphocytes
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As starting material, 5 g soil was mixed with different amounts of Bacillus subtilis cells. Sensitivity was 5 x 103 cells/5g soil.
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Images
QIAamp Spin Column procedure.
Optimized buffers lyse samples, stabilize nucleic acids, and enhance selective DNA adsorption to the QIAamp membrane. Alcohol is added and lysates loaded onto the QIAamp spin column. Wash buffers are used to remove impurities and pure, ready-to-use DNA is then eluted in water or low-salt buffer.
Apoptotic banding in stored blood.
Genomic DNA from 8 blood samples stored at 4°C for 1 week. DNA was purified using the QIAamp DNA Blood Mini Kit. When blood is stored at 4°C, the DNA is rapidly degraded due to apoptosis; the resulting apoptotic banding pattern can clearly be seen in these samples. M1: lambda-HindIII; M2:100 bp ladder.
Long-range PCR.
Amplification of a 10 kb fragment of the human ALDH1 gene from genomic DNA isolated from blood. DNA was purified using conventional methods (Phenol) or the QIAamp DNA Blood Maxi Kit (QIAamp). PCR products were sequenced directly. M: 1 kb ladder.
Paternity testing by RFLP analysis.
Three paternity cases tested with DNA purified from blood samples with the QIAamp DNA Blood Mini Kit. 1: mother; 2: child; 3: alleged father; 4: child + alleged father. M: markers. Each lane had 3 µg of DNA loaded. Outcome: A: alleged father excluded; B & C: alleged father confirmed. (Data kindly provided by J. James, Gene Proof Technologies, Nashville, TN, USA.)
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