- 对QIAGEN离心柱试剂盒,实现自动化处理
- 全自动流程,无需手工操作
- 全自动纯化DNA、RNA或蛋白
- 自动化流程,更加节省时间
- 标准化的结果,更高的工作效率
新型的QIAcube全自动核酸纯化仪采用先进的技术处理QIAGEN离心柱,将全自动、低通量样本制备无缝整合到您的实验流程中。无需更换纯化试剂,确保实验的快速启动,快速得到结果。所有纯化步骤全自动化,每批最多可处理12个样本。
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产品
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货号
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目录价:
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QIAcube (110 V)
Robotic workstation for automated purification of DNA, RNA, or proteins using QIAGEN spin-column kits: includes 1-year warranty on parts and labor
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9001292
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QIAcube System (110 V)
Robotic workstation for automated purification of DNA, RNA, or proteins using QIAGEN spin-column kits: includes installation and training, 1-year warranty on parts and labor
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9001882
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QIAcube Priority Package (110 V)
Robotic workstation for automated purification of DNA, RNA, or proteins using QIAGEN spin-column kits: includes Priority Package with software, installation, training, 2-year warranty on parts and labor, and 2 preventive maintenance visits
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9001883
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QIAcube Priority Package Plus (110 V)
Robotic workstation for automated purification of DNA, RNA, or proteins using QIAGEN spin-column kits: includes Priority Package Plus with software, installation, training, 3-year warranty on parts and labor, and 3 preventive maintenance visits
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9001884
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QIAcube (230 V)
Robotic workstation for automated purification of DNA, RNA, or proteins using QIAGEN spin-column kits: includes 1-year warranty on parts and labor
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9001293
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QIAcube System (230 V)
Robotic workstation for automated purification of DNA, RNA, or proteins using QIAGEN spin-column kits: includes installation and training, 1-year warranty on parts and labor
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9001885
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QIAcube Priority Package (230 V)
Robotic workstation for automated purification of DNA, RNA, or proteins using QIAGEN spin-column kits: includes Priority Package with software, installation, training, 2-year warranty on parts and labor, and 2 preventive maintenance visits
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9001886
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QIAcube Priority Package Plus (230 V)
Robotic workstation for automated purification of DNA, RNA, or proteins using QIAGEN spin-column kits: includes Priority Package Plus with software, installation, training, 3-year warranty on parts and labor, and 3 preventive maintenance visits
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9001887
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Starter Pack, QIAcube
200 µl filter-tips (1024); 1000 µl filter-tips (1024); 30 ml reagent bottles (12); rotor adapters (240); 1.5 ml elution tubes (240);
rotor adapter holder (1);
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990395
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QIAcube全自动核酸纯化仪仅与QIAGEN提供的、在使用手册中说明可用于QIAcube全自动核酸纯化仪的试剂盒配套使用。
Reproducible DNA yields.|High recovery of PCR products.|DNA yields comparable to the manual procedure.|Consistent yields of RNA.|Automated QIAprep Mini Standard procedure.|High PCR performance.|Highly reproducible protein purification.|Comparison of manual and automated RNA purification.|Reliable sequencing results.|Red Dot Award.|Association for Laboratory Automation’s (ALA) New Product Award (NPA)|
Human blood was collected from 12 donors and treated with EDTA. Genomic DNA was purified from 200 µl blood using the QIAamp DNA Blood Mini Kit on the QIAcube. DNA was eluted in 200 µl. Yields of genomic DNA were determined by measuring the absorbance at 260 nm. The mean values of 3 independent runs are indicated.|PCR products (2 µg in a volume of 100 µl) were purified using the QIAquick PCR Purification Kit with the manual procedure or automated on the QIAcube. Percentage recovery was determined spectrophotometrically. Recovery of PCR products using the QIAcube was comparable to the manual procedure.|pCMVβ plasmid DNA was purified from 3 ml overnight cultures of E. coli (DH5α) grown in LB medium using the QIAprep Spin Miniprep Kit automated on the QIAcube or by using the manual procedure. DNA yields were determined spectrophotometrically. Automated DNA purification on the QIAcube gave comparable yields to the manual procedure.|RNA was purified from 1 x 106 Jurkat cells using the RNeasy Mini Kit on the QIAcube. RNA yields were consistent, demonstrating the efficiency and reproducibility of the automated procedure.|Using the QIAcube. Fully automated purification of nucleic acids or proteins using QIAGEN spin kits, such as QIAprep Mini Kits, on the QIAcube eliminates the need for tedious manual steps. Sample preparation on the QIAcube follows the same steps as the manual procedure (i.e., lyse, bind, wash, and elute). No change in purification chemistry is required.|Human blood was collected from 3 donors and treated with one of 3 anticoagulants: EDTA, citrate, or heparin. Genomic DNA was purified from 200 µl blood using the QIAamp DNA Blood Mini Kit on the QIAcube. DNA was eluted in 200 µl. Yields of genomic DNA were determined by measuring the absorbance at 260 nm. A 1.2 kb fragment of the single-copy Hugl gene was amplified using 1 µl, 5 µl, or 10 µl purified DNA in a final reaction volume of 25 µl. Amplification reactions were performed using HotStarTaq DNA Polymerase. A 5 µl aliquot of each PCR was run on a 1.5% agarose gel. M: 100 bp ladder; +: positive control; –: negative control.|6xHis-tagged green fluorescent protein (GFP) was purified in duplicate from 5 ml E. coli cultures under native conditions using the Ni-NTA Spin Kit with the manual procedure or automated on the QIAcube. Proteins were visualized by Coomassie staining after SDS-PAGE. A 10 µl aliquot of eluate was loaded. M: marker.|RNA was purified using the RNeasy Mini Kit with the manual procedure or automated on the QIAcube. RNA was purified from 1 x 106 cells (RNeasy standard, RNeasy DNase digestion); 5 x 106 cells (RNeasy QIAshredder); 25 µg total RNA (RNeasy cleanup). Purified RNA was analyzed on the Agilent 2100 bioanalyzer. The high RNA Integrity Number (RIN) indicates the high quality of the RNA. |pUC19 Plasmid DNA was purified from a 1.5 ml overnight culture of E. coli grown in LB medium using the QIAprep Spin Miniprep Kit on the QIAcube and sequenced using the BigDye Terminator v3.1 Cycle Sequencing Kit. Sequencing reactions were purified using the DyeEx 96 Kit and analyzed on an ABI PRISM 3700 DNA Analyzer.|QIAcube was given the design industry’s “Red Dot Award” recognizing the platform’s innovation, functionality, quality and ergonomics.|The QIAcube - QIAGEN’s revolutionary new platform for sample preparation - receives the Association for Laboratory Automation’s (ALA) New Product Award (NPA).|
原理
QIAcube全自动核酸纯化仪能够使用成熟的QIAGEN离心柱试剂盒连续纯化核酸和蛋白,免除了繁琐的手动操作。新型的QIAcube全自动核酸纯化仪控制所有组件,包括:离心机、加热震荡器、移液体系和自动抓取器。这样QIAcube全自动核酸纯化仪能够全自动处理超过40种QIAGEN离心柱试剂盒。
QIAcube全自动核酸纯化仪预先装入各种程序,用于纯化RNA、基因组DNA、质粒DNA、病毒核酸和蛋白,以及DNA和RNA的回收。所有扩展的标准程序均可免费下载。此外,我们也可为您量身设计程序,满足您特殊的应用要求。
操作流程
QIAcube全自动核酸纯化仪采用简单的裂解、结合、洗涤和洗脱流程,纯化获得高纯度的核酸和蛋白(参见flowchart)。超过40种QIAGEN离心柱试剂盒可在QIAcube全自动核酸纯化仪上自动处理。
前所未有的易用性
QIAcube 全自动核酸纯化仪前所未有的易用性。工作站使用方便,只需简单的几个步骤,即可开始运行。无外置电脑,节省实验室空间。内置触摸屏,方便地进行程序选择;界面清楚直观,程序中提供完善的指导信息,指导使用者进行工作站设置。为了提高易用性和处理的安全性,各种耗材和配件都带有方向标记,有助于减少误操作,确保正确安放。此外,程序开始前系统进行全面的自检,检查样本、试剂和耗材是否正确安放,以确保操作能顺利完成。
新型的专用于DNA和RNA制备的试剂盒
专用的QIAcube Kits进一步简化了在QIAcube全自动核酸纯化仪上的自动离心样本制备,更加方便。目前这些试剂盒适用于纯化RNA、基因组DNA 和病毒DNA。试剂盒中提供Rotor-adapters,预装有离心柱和洗脱管,更加方便和节省时间。而且,更加容易使用,最大限度的减少使用者的失误。这些专属试剂盒的容量专为在QIAcube全自动核酸纯化仪上纯化而量身设定,避免了试剂的浪费,而且管子亦无需手动填装。
应用
QIAcube全自动核酸纯化仪非常非常适合科研实验室,以及药物研发、生物技术和生物医学研究实验室进行核酸和蛋白质的全自动提取 。
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特点
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参数
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海拔
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Up to 2000 m
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离心
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12,000 x g maximum, swing-out rotor with 12 positions, maximum 45°
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湿度
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Relative humidity of 10-75% (noncondensing)
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仪器尺寸
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650 x 620 x 570 mm
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配合仪器使用的试剂
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Most QIAGEN manual kits (e.g., QIAamp, QIAquick, QIAprep)
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运行温度
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18-28°C
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过电压保护类别
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II
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移液体系
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Syringe size 1 ml, pipetting range 5-900 µl
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操作地点
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For indoor use only
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污染水平
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2
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功率
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100-120 V AC, 50-60 Hz, 650 VA (North America and Japan), 220-240 V AC, 50-60 Hz, 650 VA (Europe), mains supply voltage fluctuations are not to exceed 10% of nominal supply voltages
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处理
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Automated
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仪器的实验方案/主要应用
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DNA purification, RNA purification, protein purification
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每次处理样本量;通量
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1–12 samples per run
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振荡器
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Speed 100-2000 rpm, amplitude 2 mm, heating range of room temperature to 70°C, ramp-up time of <5 min from room temperature to 55°C (+/-)3°C)
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软件
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QIAGEN protocols are preinstalled on the QIAcube or can be downloaded at www.qiagen.com/MyQIAcube
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储存温度
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15°C to 30°C in manufacturer's package
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仪器技术参数
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70.5 kg
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技术
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Depends on kit used
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触摸屏
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Transmissive TFT, 64 x 86 x 65 mm, white LED backlight, high brightness
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运输温度
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-25°C to 50°C in manufacturer's package
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FAQ ID -1402
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FAQ ID -1405
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FAQ ID -1406
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FAQ ID -1407
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FAQ ID -1409
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FAQ ID -1410
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FAQ ID -1411
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FAQ ID -1412
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FAQ ID -1413
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FAQ ID -1414
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FAQ ID -1415
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FAQ ID -1416
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FAQ ID -1418
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FAQ ID -1419
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FAQ ID -1421
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FAQ ID -1422
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FAQ ID -1423
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FAQ ID -2337
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FAQ ID - 3479
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FAQ ID - 3482
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FAQ ID -1403
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FAQ ID -3054
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Search for QIAcube Standard Protocols
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