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Rotor-Gene Q

表现卓越的定量PCR

  • 转子式设计,确保卓越的热力学和光学性能
  • 无与伦比的光学范围,波长从紫外到红外
  • 界面友好的软件,支持领先的分析
  • 强大的设计,确保最低的维护需求及最大的方便性
  • 配合QIAGEN分析技术,确保结果可靠的多重应用

QIAGEN的Rotor-Gene Q实时荧光定量PCR分析仪结合多种优化的设计特性,具有卓越的性能,确保结果可靠。配合经优化的QIAGEN real-time PCR试剂盒,Rotor-Gene Q实时荧光定量PCR分析仪可进行简单的分析,用于多种领域。

Cat No./ID: 9001550
Rotor-Gene Q 2plex Platform
Real-time PCR cycler with 2 channels (green, yellow), laptop computer, software, accessories: includes 1-year warranty on parts and labor, installation and training not included
Cat No./ID: 9001560
Rotor-Gene Q 2plex HRM Platform
Real-time PCR cycler and High Resolution Melt analyzer with 2 channels (green, yellow) plus HRM channel, laptop computer, software, accessories: includes 1-year warranty on parts and labor, installation and training not included
Cat No./ID: 9001570
Rotor-Gene Q 5plex Platform
Real-time PCR cycler with 5 channels (green, yellow, orange, red, crimson), laptop computer, software, accessories: includes 1-year warranty on parts and labor, installation and training not included
Cat No./ID: 9001580
Rotor-Gene Q 5plex HRM Platform
Real-time PCR cycler and High Resolution Melt analyzer with 5 channels (green, yellow, orange, red, crimson) plus HRM channel, laptop computer, software, accessories: includes 1-year warranty on parts and labor, installation and training not included
Cat No./ID: 9001590
Rotor-Gene Q 6plex Platform
Real-time PCR instrument with 6 channels (blue, green, yellow, orange, red, crimson), including laptop computer, software, accessories: includes 1-year warranty on parts and labor, installation and training not included
Cat No./ID: 9001620
Rotor-Gene Q 2plex System
Real-time PCR cycler with 2 channels (green, yellow), laptop computer, software, accessories: includes 1-year warranty on parts and labor, installation and training
Cat No./ID: 9001630
Rotor-Gene Q 2plex HRM System
Real-time PCR cycler and High Resolution Melt analyzer with 2 channels (green, yellow) plus HRM channel, laptop computer, software, accessories: includes 1-year warranty on parts and labor, installation and training
Cat No./ID: 9001640
Rotor-Gene Q 5plex System
Real-time PCR cycler with 5 channels (green, yellow, orange, red, crimson), laptop computer, software, accessories: includes 1-year warranty on parts and labor, installation and training
Cat No./ID: 9001650
Rotor-Gene Q 5plex HRM System
Real-time PCR cycler and High Resolution Melt analyzer with 5 channels (green, yellow, orange, red, crimson) plus HRM channel, laptop computer, software, accessories: includes 1-year warranty on parts and labor, installation and training
Cat No./ID: 9001660
Rotor-Gene Q 6plex System
Real-time PCR instrument with 6 channels (blue, green, yellow, orange, red, crimson), including laptop computer, software, accessories: includes 1-year warranty on parts and labor, installation and training
Cat No./ID: 9001861
Rotor-Gene Q 2plex Priority Package
Real-time PCR cycler with 2 channels (green, yellow), laptop computer, software, accessories: includes Priority Package with software, installation, training, 2-year warranty on parts and labor, and 2 preventive maintenance visits
Cat No./ID: 9001862
Rotor-Gene Q 2plex Priority Package Plus
Real-time PCR cycler with 2 channels (green, yellow), laptop computer, software, accessories: includes Priority Package with software, installation, training, 3-year warranty on parts and labor, and 3 preventive maintenance visits
Cat No./ID: 9001863
Rotor-Gene Q 2plex HRM Priority Package
Real-time PCR cycler and High Resolution Melt analyzer with 2 channels (green, yellow) plus HRM channel, laptop computer, software, accessories: includes Priority Package with software, installation, training, 2-year warranty on parts and labor, and 2 preventive maintenance visits
Cat No./ID: 9001864
Rotor-Gene Q 2plex HRM Priority Package Plus
Real-time PCR cycler and High Resolution Melt analyzer with 2 channels (green, yellow) plus HRM channel, laptop computer, software, accessories: includes includes Priority Package with software, installation, training, 3-year warranty on parts and labor, and 3 preventive maintenance visits
Cat No./ID: 9001865
Rotor-Gene Q 5plex Priority Package
Real-time PCR cycler with 5 channels (green, yellow, orange, red, crimson), laptop computer, software, accessories: includes Priority Package with software, installation, training, 2-year warranty on parts and labor, and 2 preventive maintenance visits
Cat No./ID: 9001866
Rotor-Gene Q 5plex Priority Package Plus
Real-time PCR cycler with 5 channels (green, yellow, orange, red, crimson), laptop computer, software, accessories: includes Priority Package with software, installation, training, 3-year warranty on parts and labor, and 3 preventive maintenance visits
Cat No./ID: 9001867
Rotor-Gene Q 5plex HRM Priority Package
Real-time PCR cycler and High Resolution Melt analyzer with 5 channels (green, yellow, orange, red, crimson) plus HRM channel, laptop computer, software, accessories: includes Priority Package with software, installation, training, 2-year warranty on parts and labor, and 2 preventive maintenance visits
Cat No./ID: 9001868
Rotor-Gene Q 5plex HRM Priority Package Plus
Real-time PCR cycler and High Resolution Melt analyzer with 5 channels (green, yellow, orange, red, crimson) plus HRM channel, laptop computer, software, accessories: includes Priority Package with software, installation, training, 3-year warranty on parts and labor, and 3 preventive maintenance visits
Cat No./ID: 9001869
Rotor-Gene Q 6plex Priority Package
Real-time PCR instrument with 6 channels (blue, green, yellow, orange, red, crimson), including laptop computer, software, accessories: includes Priority Package with software, installation, training, 2-year warranty on parts and labor, and 2 preventive maintenance visits
Cat No./ID: 9001870
Rotor-Gene Q 6plex Priority Package Plus
Real-time PCR instrument with 6 channels (blue, green, yellow, orange, red, crimson), including laptop computer, software, accessories: includes Priority Package with software, installation, training, 3-year warranty on parts and labor, and 3 preventive maintenance visits
Cat No./ID: 9020147
Rotor-Gene ScreenClust HRM Software
Software CD, user guide

Rotor-Gene Q实时荧光定量PCR分析仪应与相应的QIAGEN试剂盒配合使用,适用于试剂盒用户手册中指定的应用。如果将Rotor-Gene Q实时荧光定量PCR分析仪与非QIAGEN的试剂盒配合使用,用户应自行确认是否适用于相关应用。



0
检测IV类SNP。
使用野生型(蓝色)、基因纯合缺失型(绿色)和杂合子(红色)样本的基因组DNA分析人AHRR7基因的A/T SNP。使用Type-it HRM PCR Kit和带有HRM功能的Rotor-Gene Q实时荧光定量PCR分析仪进行实验。使用非监督模式的Rotor-Gene ScreenClust HRM Software进行数据分析。A/T多态性(IV类SNPs)是最难区分的,因为纯合子等位基因型的差异很小(该例中少于0.1°C)。[A] HRM原始数据,[B]聚类情况。所有未知的类型根据基因表型进行正确分类。
1
准确的real-time PCR分析。
用2倍稀释的人基因组DNA,从30 ng(10,000 拷贝)到0.06 ng(20拷贝 ,作为模板进行real-time PCR。使用自行设计的TaqMan探针和Rotor-Gene Probe PCR Kit在Rotor-Gene Q实时荧光定量PCR分析仪上对IL1R2进行分析,每个浓度重复五次实验。相邻浓度模板间的CT值平均差1.07个循环。人基因组DNA作为模板使用自行设计的TaqMan探针在Rotor-Gene Q实时荧光定量PCR分析仪上对BCL2进行72次重复的real-time PCR,无需ROX标准化。平均CT值24.94,标准偏差只有0.05,相当于CV为0.2%。
2
Highly sensitive detection of methylated DNA.
Mixtures of methylated and unmethylated DNA (EpiTect Control DNA) of varying ratios were used as template. A CpG island from the promoter region of the APC gene (adenomatosis polyposis coli) was amplified and the degree of methylation was determined by HRM methylation analysis on the Rotor-Gene Q using the EpiTect HRM PCR Kit. [A] A standard normalized melt curve and a [B] difference plot normalized to the 50% methylated sample are shown.
3
Rotor-Gene Q实时荧光定量PCR分析仪剖面图。
加热/冷却通过在反应箱中的快速气流实现。每150 ms管子旋转通过激发/检测光,能够高速捕获数据。6个分开的LED光源能够与6个不同的检测滤波器和1个灵敏的光电倍增检测器配合使用。
4
灵活的规格。
The Rotor-Gene Q supports multiple formats to suit a range of needs. Choose between tubes or Rotor-Discs, which offer accelerated setup and high throughput. Change the format in seconds by simply switching the snap-fit metal rotor that holds your plasticware format of choice.
Performance
Rotor-Gene Q实时荧光定量PCR分析仪是目前唯一一台可通过HRM分析最高难度级,IV级别SNPs的real-time PCR仪。QIAGEN专为您提供HRM试剂盒,用于HRM的各种应用,如基因分型(参见"Identification of a class IV SNP" for data from the Type-it HRM PCR Kit),定量甲基化分析(参见"Highly sensitive detection of methylated DNA" for data from the EpiTect HRM PCR Kit),基因扫描和序列匹配。Type-it HRM PCR Kit支持可靠、准确基因突变和SNP的检测。EpiTect HRM PCR Kit可实现快速筛查和精确检测亚硫酸氢盐转化后DNA的CpG甲基化水平的变化。
Principle
独特的转子设计,卓越的性能

Rotor-Gene Q实时荧光定量PCR分析仪独特的离心转子式设计使其成为当前最精确的多功能定量PCR仪(参见"Cross-section of the Rotor-Gene Q")。 反应仓中各离心管在快速热循环过程中能够保持相同的温度,光学检测具有同样的均一性。每个试管与检测光成一线时,会激发样本,并通过单个短光路快速收集到荧光信号。均一的热性能和光学性能确保灵敏和准确的定量PCR分析(参见"Precise real-time PCR analysis")。 避免了传统板式PCR仪无法避免的样本间差异及边缘效应。

转子式设计确保下列优势:

管间温差小于±0.02°C
均一检测,无需使用ROX校正染料
快速升降温和可忽略不计的平衡时间,缩短了反应时间
完全可靠的结果
无与伦比的光学范围,适合多种应用

无论分析是基于如SYBR® Green的内参染料、如水解(TaqMan)的探针法还是杂交(FRET)探针、Scorpion探针,或多种化学试剂,Rotor-Gene Q实时荧光定量PCR分析仪都能满足所有需求。该仪器提供多至6个通道,波长从紫外到红外,是当前光谱范围最广的PCR仪(见表)。此外,应用相应的软件可获得新的激发/检测光波长组合,这就意味着Rotor-Gene Q实时荧光定量PCR分析仪与将来可能使用的染料兼容。

光学检测通道
通道 激发(nm) 检测(nm) 适用的荧光素举例
365 ± 20 460 ± 20 Marina Blue, Edans, Bothell Blue,
Alexa Fluor 350, AMCA-X
绿 470 ± 10 510 ± 5 FAM, SYBR® Green I, Fluorescein,
EvaGreen, Alexa Fluor 488
530 ± 5 557 ± 5 JOE, VIC, HEX, TET, MAX, CAL Fluor Gold 540, Yakima Yellow
585 ± 5 610 ± 5 ROX, CAL Fluor Red 610, Cy 3.5,
Texas Red, Alexa Fluor 568
625 ± 5 660 ± 10 Cy5, Quasar 670, LightCycler
Red640, Alexa Fluor 633
深红 680 ± 5 712 high pass Quasar 705, LightCycler Red705,
Alexa Fluor 680
HRM 460 ± 20 510 ± 5 SYBR® Green I, SYTO9, LC Green,
LC Green Plus+, EvaGreen
使用HRM,扩展您的研究

High-resolution melting analysis (HRM)作为一种闭管式的PCR后分析技术,引起了学术界的广泛关注。HRM基于DNA双链的熔解特性对PCR产物进行分析,与传统的熔解曲线分析类似,但提供更多的信息,因而具有更广泛的应用。可根据PCR产物的序列、长度、GC含量或链互补性以及单个碱基对的变化来区分,其灵敏度可区分单个碱基对的差别。现在可以通过这种高效、简单的方法检测、分析未知的甚至复杂的序列。Rotor-Gene Q实时荧光定量PCR分析仪的转子式设计和出色的热性能及光学表现使其高度适用于HRM。

Rotor-Gene Q实时荧光定量PCR分析仪的HRM选项包括:

特殊调校的高强度HRM光学通道
温度分辨率为0.02°C
快速采集数据
功能全面的HRM软件
最少的维护需求,最大的方便性

Rotor-Gene Q实时荧光定量PCR分析仪的设计降低了维护需求,使用方便。节约时间和成本,让您可以将更多精力集中于科研工作,而不是围着机器。

Rotor-Gene Q实时荧光定量PCR分析仪的方便性如下:

高度稳定的LED光源,保证其寿命,无需更换光源或激光器,避免光学性能逐渐降低
安装时及仪器移动后无需光学校正
无需清洁孔板
不存在旋转引起的冷凝和气泡
小巧、轻便、功能强大,可随意放置仪器
Procedure
灵活的规格,满足您的需求

Rotor-Gene Q实时荧光定量PCR分析仪支持多种规格的PCR管,满足各种需求。只需更换承载PCR管的金属转子,便可轻松更换规格。除了单管,也提供加快反应体系构建、提高通量的Rotor-Disc。Rotor-Disc是环状板,反应孔为垂直方向。Rotor-Disc 100相当于一个96孔板加4个参照管。这些多出的反应孔可用于更多反应或参照。也提供72孔的Rotor-Disc 72。使用Rotor-Disc Heat Sealer可快速方便的用塑料膜密封Rotor-Disc。所有需要应用Rotor-Disc运行的反应,选择Rotor-Disc 100 Starter Kit或Rotor-Disc 72 Starter Kit。

可手动构建反应体系,或使用QIAGEN自动化方案自动构建反应体系。QIAgility可经济、快速、高速准确的构建反应体系,同时QIAsymphony AS是进行常规日间PCR差异检测的理想选择。所有仪器可在Rotor-Gene模式下自动构建反应体系,可直接传递样本清单,并为各种不同的实验方案提供定量PCR预混液。

简单的常规验证

实验室经常需要进行常规的性能精确性验证。对于大部分的PCR仪,这个过程需要工程师的介入。而对于Rotor-Gene Q实时荧光定量PCR分析仪,这不是必需的。使用简单、价格合理的Rotor-Disc OTV(Optical Temperature Verification)Kit可自动进行精确性检测。几分钟内完成整个流程。

Applications

一系列可用于Rotor-Gene Q实时荧光定量PCR分析仪的QIAGEN试剂盒能够在所有定量PCR应用中可靠定量,无需优化反应和循环条件。用于定量PCR和HRM应用的试剂盒,适合多种应用:

基因表达分析 
病原体检测
基因分型和基因扫描
miRNA研究
Software
软件支持定量,提高数据的安全性

功能全面的Rotor-Gene Q实时荧光定量PCR分析仪软件包支持当前领先的定量分析流程,涵盖了所有基本和高级的数学运算法则。您可以完全自主地分析宝贵的数据,提高结果的可靠性。软件确保数据安全,从运行开始到结果输出全程都可追踪。

更先进的软件,采用HRM分析用于基因分型和突变检测

Rotor-Gene ScreenClust HRM Software是Rotor-Gene运行软件的扩展。该软件是当前功能较强大的工具,适合分析从Rotor-Gene Q实时荧光定量PCR分析仪或Rotor-Gene 6000循环仪获得的HRM数据。对于一组样本的分析,Rotor-Gene ScreenClust HRM Software打开了一个新的模块,用于如基因分析和突变检测等HRM分析应用。

Features
Specifications
海拔 Up to 2000 m (6500 ft.)
尺寸 Width, 37 cm (14.6 in.); Height, 28.6 cm (11.3 in.); Depth (without cables), 42 cm (16.5 in.); Depth (door open), 53.8 cm (21.2 in.)
特点 Dynamic range, 12 orders of magnitude
散热/热负荷 Average, 0.183 kW (632 BTU/hour); Peak, 0.458 kW (1578 BTU/hour)
湿度 10–75% (noncondensing)
配合仪器使用的试剂 artus QS-RGQ Kits (not available in all countries), RG SYBR Green PCR Kits; RG SYBR Green RT-PCR Kit; RG Probe PCR Kits; RG Probe RT-PCR Kit; RG Multiplex PCR Kit
运行温度 18-30ºC (64-86ºF)
光学系统 Up to 6 channels spanning UV to infra-red wavelengths; Excitation sources: High energy light-emitting diodes; Detector: Photomultiplier; Acquisition time: 4 s. The software allows to create new excitation/detection wavelength combinations
过电压保护类别 II
操作地点 For indoor use only
污染水平 2; Environmental class 3K2 (IEC 60721-3-3)
功率 100–240 V AC, 50–60 Hz, <520 VA (peak); Power consumption <60 VA (standby); Mains supply voltage fluctuations are not to exceed 10% of the nominal supply voltages; F5a 250 V fuse
仪器的实验方案/主要应用 Gene expression analysis, microRNA detection, Virus detection, SNP genotyping, SNP genotyping, High Resolution Melt analysis (HRM)
每次处理样本量;通量 Tubes 0.2 ml; Strip Tubes 0.1 ml (4 tubes); Rotor-Disc 72; Rotor-Disc 100; Up to 100 samples per run using a Rotor-Disc 100
软件 Rotor-Gene Q software, supplied on the installation CD provided
储存条件 15ºC to 30ºC (59ºF to 86ºF) in manufacturer’s package; Max. 75% relative humidity (noncondensing); Environmental class 1K2 (IEC 60721-3-1)
技术 Real-time PCR cycler
热力性能 Temperature range, 35 to 99ºC; Temperature accuracy, ±0.5ºC (type, measured 30 seconds after clock start); Temperature resolution, ±0.02ºC (smallest programmable increment); Temperature uniformity, ±0.02ºC; Ramp rate (peak ramp rates, air), >15ºC/s heating, >20ºC/s cooling
运输条件 –25ºC to 60ºC (–13ºF to 140ºF) in manufacturer’s package; Max. 75% relative humidity (noncondensing); Environmental class 2K2 (IEC 60721-3-2)
常规运行时间 40 cycles in 45 min with the QIAGEN RG Kits (assay dependent)
质保 1 year on instrument; lifetime guarantee on excitation LEDs
重量 12.5 kg (27.6 lb.), standard configuration

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常见问题 (48)
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操作软件 (2)
For use on the Rotor-Gene Q. Rotor-Gene Q Software 2.3 is compatible with both Windows XP and Windows 7 operating systems. If you would like to upgrade the operating system for your QIAGEN computer (HP ProBook 6550b or HP ProBook 6570b) to Windows 7, please contact QIAGEN Technical Services for your Windows 7 image CD.
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分析软件 (6)

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For use with Q-Rex Software version 1.0 or higher to import sample information from QIAgility instruments
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New Gene Expression Analysis Plug-in for the Q-Rex Software
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For use with the Q-Rex Software v1.0 to calculate absolute concentration of targets by PCR
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New target detection analysis plug-in for the Q-Rex Software
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For use with the Q-Rex Software v1.0 to calculate absolute concentration of targets by PCR. The plug-in is dedicated to our customers working with absolute quantification HID experiments.
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用户使用手册 (4)
For use with Rotor-Gene Q real-time cyclers
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Rotor-Gene Q User Manual, version 5, for Rotor-Gene Q instruments using Rotor-Gene Q software version 2.3.1, or higher
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For use with Rotor-Gene Q instruments and Q-Rex Software version 1.0
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软件使用指南 (6)
For use with the Q-Rex Software v1.0 to determine quantification cycle values
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For use with Q-Rex Software version 1.0 to perform relative quantitation analyses
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For use with the Q-Rex Software version 1.0 to detect targets by PCR
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For use with Q-Rex Software version 1.0 to calculate absolute concentration of targets by PCR
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For use with Q-Rex Software version 1.0 to calculate absolute concentration of targets by PCR. This user manual provides information about the functions and features of the Q-Rex Absolute Quantification HID plug-in.
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For use with Rotor-Gene Q instruments and Q-Rex Software version 1.0
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