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QIAgility

快速地自动化构建PCR体系,具有高度的精确性
  • 各种模式的PCR体系自动化构建
  • 方便、易于使用的软件
  • 标准化结果、提高产量
  • 无需手工移液
  • 与QIAGEN样本制备和分析技术的无缝整合

QIAgility是一款结构紧凑的仪器,可快速、高度准确的进行PCR体系构建。QIAgility功能强大,支持几乎所有规格的试管和孔板,以及Rotor-Gene Q实时荧光定量PCR分析仪的Rotor-Disc。QIAgility通过安装有操作软件的电脑操作。为了PCR反应一次成功,可使用经验证的Q Protocols构建PCR反应体系,配合用于终点法和real-time PCR的QIAGEN PCR试剂盒和酶。

Cat No./ID: 9001532
QIAgility System HEPA/UV (incl. PC)
Robotic workstation for automated PCR setup (with UV light and HEPA filter), notebook computer, and QIAgility Setup Manager Software: includes installation and training, 1-year warranty on parts and labor
Cat No./ID: 9001903
QIAgility Priority Package HEPA/UV (incl. PC)
Robotic workstation for automated PCR setup (with UV light and HEPA filter), notebook computer, and QIAgility Setup Manager Software: includes Priority Package with software, installation, training, 2-year warranty on parts and labor, and 2 preventive maintenance visits
Cat No./ID: 9001904
QIAgility Priority Package Plus HEPA/UV (incl. PC)
Robotic workstation for automated PCR setup (with UV light and HEPA filter), notebook computer, and QIAgility Setup Manager Software: includes Priority Package with software, installation, training, 3-year warranty on parts and labor, and 3 preventive maintenance visits

QIAgility适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


0
Exceptional pipetting precision and accuracy spanning over a broad volume range
The Artel MVS system was employed to determine the pipetting precision of the QIAgility instrument operated by the QIAgility Setup Manager Software. The QIAgility dispensed the Artel solutions (selected according to the Artel MVS User Manual) into the Artel buffer. Three runs were performed on three QIAgility instruments that were used to pipet an entire volume range (1, 2, 5, 10, 20, 50, 100, and 200 µl), in replicates of 12. For each run and volume (n=12) the coefficient of variation (CV) was calculated to determine the pipetting precision of the QIAgility. The data shows the mean of the CV values (bars; in %) ± standard deviation (S.D.) of the CV values as error bars. The red lines depict the claimed specifications.
The indicated CV values (%) demonstrate that the QIAgility instrument performs within its claimed specifications and even better.
1
Unrivaled pipetting precision and reproducibility of the QIAgility for master mix solutions
A reaction mix was prepared manually using 5 parts of 2x Rotor-Gene SYBR Green PCR Master Mix and 3 parts of RNase free water (ready-made reaction mix). An experiment was then defined in the QIAgility Setup Manager for the Rotor-Gene SYBR Green PCR Kit with a total reaction volume of 25 µl and a sample input volume of 5 µl, resulting in the transfer of 20 µl of the ready-made reaction mix. The transfers were made into pre-weighed 200 µl single tubes (n=36 per instrument). The tubes were weighed again after the transfer and the weight difference was considered to calculate the transferred volume. For each instrument, the pipetting precision was determined as CV (%). The data shows the mean of the CV values (bars; in %) ± S.D. (error bars) of each individual instrument. The pipetting precision or CV, respectively, was nearly 3 % for all instruments tested.
The indicated CV values (%) demonstrate that the QIAgility instrument has high performance rate in terms of precision and repeatability when pipetting ready-made reaction mix as shown on all 4 instruments.
2
Comparison of manual and automated assay setup using series dilutions
A QuantiNova SYBR Green assay setup was performed using the QIAgility operated with its Setup Manager Software and this was compared to a manual setup done in parallel. A manually prepared 1:10 dilution series from a stock of human gDNA with a concentration of 60 ng/µl was used in a ß-actin assay. Each dilution was tested in quadruplicates (n=4). The manual and QIAgility-prepared assay points were amplified in the same Rotor-Gene Q run. The data is represented as a box plot of the CT value calculated using the Q-Rex Software for each template concentration and setup employed.
A highly similar PCR performance is observed between manual and automated assay setup over a series of sample concentrations.
3
High system linearity of QIAgility
Human gDNA with a stock concentration of 1.5 µg/µl was diluted with the QIAgility six times in the ratio of 1:2 per dilution step using RNase free water as diluent. The dilution series was prepared in replicates of 3. The nucleic acid concentration of the dilutions was determined using the QIAxpert UV/VIS spectrophotometer (DNA QIAamp Application). The mean measured quantifications were plotted against the mean theoretical nucleic acid concentrations assuming a perfect 1:2 dilution. The resulting plot demonstrates the exceptional linearity of QIAgility dilution with a high regression coefficient of >0.999.
Quantifications of serial dilutions of gDNA prepared using the QIAgility are in high concordance with actual concentrations.
Performance

自动化的PCR反应体系构建快速、可靠,同时避免人为移液引起的操作误差。

QIAgility先进的设计确保流程安全性,让您无后顾之忧。仪器具备内置的感应器,一旦运行期间机罩打开,仪器就会暂停,保护用户不会因为移动零件受伤。可选择紫外灯对工作台进行高效灭菌,还可选择HEPA过滤器在PCR反应体系构建期间提供洁净的空气。当错误出现时,软件就会通过安全警告启动安全措施。

除了PCR体系构建,凭借QIAgility灵活的功能用户可设计高度精确的流程,具有广泛的适用性,包括:

  • 标准化DNA和RNA的浓度
  • 从一种规格的试管中转移液体样本到另一规格的试管
  • 获得各种稀释比例的稀释液
  • 限制性酶切反应构建
  • 样本混合
  • 从样本库中选择性移取
Principle

QIAgility应用单通道移液进行自动化PCR反应体系构建,可在30分钟内设置多至96个PCR反应。传导性滤芯吸头便于液面感应,确保高度精确、标准化的移液。自动化PCR反应体系构建快速可靠,避免了易于出错的人工移液。QIAgility可将可靠的QIAGEN PCR、RT-PCR、定量PCR和定量RT-PCR试剂盒全自动化。QIAgility可构建多种预混液,同时构建多种PCR反应体系。

QIAgility先进的设计确保流程安全性,让您无后顾之忧。仪器具备内置的感应器,一旦运行期间机罩打开,仪器就会暂停,保护用户不会因为移动零件受伤。可选择紫外灯对工作台进行高效灭菌,还可选择HEPA过滤器在PCR反应体系构建期间提供洁净的空气。当错误出现时,软件就会通过安全警告启动安全措施。

Procedure
仪器工作台有一个Master Mix Block、一个Reagent Block、吸头和孔板/试管。QIAgility软件模拟仪器工作台,无论是自动化新手还是有经验的实验人员都可方便使用。只需选择一个程序,设置工作台,即可实现全自动PCR体系构建。样本可直接用于下游应用。
Applications

QIAgility高度适用于进行PCR和RT-PCR的实验室:

基因表达分析
病毒检测
基因分型
克隆
病原体检测 
Software
QIAgility Software模拟仪器工作台,使用极其方便(参见"Easy-to-use software")。向导会通过软件的逐步引导,帮助用户快速构建运行文档。用户也可下载Q Protocols并根据需求对其进行修正。Q Protocols是由QIAGEN开发的运行文档,直接用于特定PCR扩增的构建。该软件在移液管移动时会在屏幕上显示它的位置,可实时追踪运行过程。
Services
Is your plate or tube not supported in the QIAgility software? Contact QIAGEN Technical Services to request a new plate or tube to suit your workflow.
Features
Specifications
提供的附件 2 x Adapter, 96 x 0.2 ml PCR Tubes 1 x Adapter, Flip-Cap 32 x 1.5 ml Tapered Base 1 x Adapter, 72 x 0.1 ml Strip Tubes 1 x Master Mix Block, 5 x 5 ml 1 x Master Mix Block, 1 x 5 ml, 4 x 2 ml 1 x Master Mix Block, 1 x 5 ml, 4 x 1.5 ml 1 x Reagent Block, 16 x 0.2 ml / 2 ml Flat / 1.5 ml Tapered Tubes 4 x Tip Rack Holder for 96-Tip Rack The adapters are intended to hold tubes or plates.
交流 USB and RS232
导电滤芯 50 µl and 200 µl Conductive Filtered Tips for liquid-level sensing
提供的耗材 10 racks of 50 µl Conductive Filtered Tips (960); 10 racks of 200 µl Conductive Filtered Tips (960); 25 x 5 ml tubes; 100 x 0.2 µl PCR tubes; 3 Tip Disposal Boxes
尺寸 Height: 45 cm (18 in.), instrument lid closed; Height: 86 cm (34 in.), instrument lid open; Width: 54 cm (21 in.); Depth: 63 cm (25 in.), without cables
电动 100–240 V AC, 250 VA, 50–60 Hz
HEPA过滤器 Preinstalled dual-cartridge 3M Air-Mate HEPA (High Efficiency Particulate Air) filter system provides positive pressure clean air within the closed hood.
湿度 Operating humidity: 40–70%
液位感应 Minimum detection volume (10 µl in 200 µl PCR tubes). Only functional in combination with QIAGEN Conductive Filtered Tips.
提供笔记本电脑 QIAgility comes with a notebook computer for added flexibility.
移液吸头 Single channel
操作地点 For indoor use only
污染水平 2
精确度 CV <1%, 5–200 µl (dry-well transfer) CV <5%, 2–5 µl (wet-well transfer) CV <10%, 1 µl
软件 Supplied with unlimited user license. Free upgrades by Web download.
表容量 6 x SBS positions (127.76 x 85.48 mm) for adapters and Tip Rack Holders 1 x Master Mix Block 1 x Reagent Block
温度 Operating temperature, 15–30 °C (59–86°F)
弃去吸头 All used tips ejected externally — no waste accumulation on the worktable
吸头容量 1–200 µl
紫外灯 UV lamp for worktable sterilization. Available only with initial purchase, in combination with the HEPA filter.
质保 1-3 years warranty, depending on service package
重量 41 kg (90.2 lb)

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用户使用手册 (4)
Important note regarding new QIAgility declaration of conformity and instrument symbols
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For use with QIAgility Operating Software version 4.17.1
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For use with QIAgility and QIAgility Setup Manager Software
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实验方案软件 (1)
These protocol files can only be used with QIAgility Operating Software version 4.17 and are not compatible with the new QIAgility Setup Manager Software.
QIAgility Setup Manager Software offers a complete solution for qPCR setup optimized for use with QIAGEN kits. Please click here to view the list of kits currently supported by the QIAgility Setup Manager Software.

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操作软件 (2)
Instrument operating software designed for flexible use in various applications. Use this software for all applications other than gene expression and pathogen detection. For gene expression and pathogen detection applications, we recommend using the QIAgility Setup Manager Software.

Important
: It is not possible to install both QIAgility Operating Software 4.17.1 and QIAgility Setup Manager Software on the same computer. Please uninstall QIAgility Setup Manager Software before installing QIAgility Operating Software 4.17.1. For more information, contact QIAGEN Technical Services.

Note
: QIAgility Operating Software is not compatible with CAS-1200 instruments. Download the latest CAS-1200 operating software.
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Instrument operating software including the QIAgility Setup Manager Gene Expression, Pathogen Detection and Sample Pooling plug-in. This new software offers a complete solution for qPCR setup and is optimized for use with QIAGEN gene expression and pathogen detection kits, such as QuantiTect, QuantiFast, QuantiNova and Rotor-Gene kits. We recommend using this software for all gene expression, pathogen testing and sample pooling applications.

For software installation instructions, please refer to the QIAgility Unpacking and Installation Instructions or to the QIAgility User Manual, chapter 4.4.1.

Important
: It is not possible to install both QIAgility Setup Manager Software and QIAgility Operating Software 4.17.1 on the same computer. Please uninstall QIAgility Operating Software 4.17.1 before installing QIAgility Setup Manager Software. For more information, contact QIAGEN Technical Services.

Note
: QIAgility Operating Software is not compatible with CAS-1200 instruments. Download the latest CAS-1200 operating software.
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