PyroMark Q96 Tests

For analyzing gene-specific sequence variation or methylation on PyroMark Q96 ID or PyroMark Q96 MD

Detection and quantification of mutations in cancer-related genes
Quantification of individual or multiple CpG sites in different genes
Estimation of global CpG methylation in defined regions of interest
Sequence context provides built-in quality control of the assays

PyroMark Q96 Tests are a range of predesigned assays suitable for highly reliable, quantitative analysis of mutations or SNPs, as well as CpG methylation. Tests designated specifically with 'Q96' are optimized for use with the PyroMark Q96 MD and/or PyroMark Q96 ID Instruments. Other tests, such as PyroMark HFE and PyroMark MTHFR, can additionally be performed on the PyroMark Q24 (with the exception of PyroMark APOE).

订购信息
详细信息
相关资料

请登录账号查看购物车

产品	货号	目录价：
PyroMark APOE (96) For detection of polymorphism in the APOE gene (2 x 96 reactions on PyroMark Q96 MD or 1 x 96 reactions on PyroMark Q96 ID)	972422	询价
PyroMark HFE (96) For detection of variants in the HFE gene (2 x 96 reactions on PyroMark Q96 MD, 1 x 96 reactions on PyroMark Q96 ID, 8 x 24 reactions on PyroMark Q24)	972442	询价
PyroMark MTHFR (96) For detection of variants in the MTHFR gene (2 x 96 reactions on PyroMark Q96 MD, 1 x 96 reactions on PyroMark Q96 ID, 8 x 24 reactions on PyroMark Q24)	972432	询价
PyroMark Q96 CpG LINE-1 (2 x 96) For quantification of global methylation level in transposable elements; only for use with PyroMark Q96 MD	973043	询价
PyroMark Q96 CpG MGMT (96) For quantification of methylation level in the MGMT gene (2 x 96 reactions on PyroMark Q96 MD and 1 x 96 reactions on PyroMark Q96 ID)	972032	询价
PyroMark Q96 CpG MLH1 (96) For quantification of methylation level in promoter region of the MLH1 gene (2 x 96 reactions on PyroMark Q96 MD and 1 x 96 reactions on PyroMark Q96 ID)	972022	询价
PyroMark Q96 CpG p16 (96) For quantification of methylation level in the p16 gene (2 x 96 reactions on PyroMark Q96 MD and 1 x 96 reactions on PyroMark Q96 ID)	972012	询价
PyroMark Q96 CpG Prader-Willi (96) For quantification of methylation level in Prader-Willi and Angelman Syndromes (2 x 96 reactions on PyroMark Q96 MD and 1 x 96 reactions on PyroMark Q96 ID)	972052	询价

请登录账号查看购物车

PyroMark APOE、PyroMark HFE、PyroMark MTHFR、PyroMark Q96 CpG LINE-1、PyroMark Q96 CpG MGMT、PyroMark
Q96 CpG MLH1、PyroMark Q96 CpG p16和PyroMark Q96 CpG Prader-Willi检测适用于分子生物学应用，不适用于疾病的诊断、预防或治疗。

PyroMark p16分析。|

对[A]正常的血液DNA样本和[B]从结肠肿瘤中纯化的DNA样本进行分析。蓝色柱表示检测的CpG位点，以及每个位点的甲基化程度。黄色柱表示完全亚硫酸氢盐转化的质控。|

性能

The PyroMark Q96 Tests are highly suitable for accurately measuring the CpG methylation status, as exemplified with the PyroMark Q96 CpG p16 Test (see figure PyroMark p16 analysis).

原理

PyroMark Q96 Tests used in combination with PyroMark Q96 MD and/or PyroMark Q96 ID Systems enable accurate, quantitative mutation (or SNP) and methylation analysis by Pyrosequencing. The assays consist of PCR primers and a sequencing primer.

操作流程

Prior to Pyrosequencing, a biotinylated PCR product is generated. Biotinylated PCR products are bound to streptavidin-coated Sepharose beads. The beads are then captured with the vacuum tool on the PyroMark Vacuum Workstation, and throroughly washed, generating single-stranded DNA suitable for sequencing. PyroMark Q96 Tests are predesigned assays for methylation analysis by Pyrosequencing. Each test includes the primers necessary for generating the biotinylated PCR product, as well as the primer for the Pyrosequencing reaction.

应用

PyroMark Q96 Tests enable highly accurate, quantitative methylation and mutation (or SNP) analysis applicable in several fields of research, including cancer and epigenetics.

常见问题

分类选项按字母顺序排序
	Can I order the nucleotides from PyroMark Gold Reagents separately? FAQ ID -2827	浏览
	Which purity grade is recommended for pyrosequencing primers? FAQ ID -2832	浏览
	How many times can vacuum troughs be re-used with the PyroMark Vacuum Preparation Stations? FAQ ID -2848	浏览
	Where can I order the Streptavidin Sepharose beads for pyrosequencing? FAQ ID -2850	浏览
	What is the amplicon length of the PyroMark CpG LINE assay? -1	浏览
	What region of LINE gets targeted by the assay for PyroMark Q96 MD or PyroMark Q24? -1	浏览
	How many CpG sites are analyzed by the PyroMark CpG LINE assay? -1	浏览
	Does the PyroMark CpG LINE assay target mouse transposons as well? -1	浏览
	Does the PyroMark LINE assay target all LINE sequences? -1	浏览
	Can the PyroMark Q96 CpG LINE assay be used with an ID system? -1	浏览
	How many nucleotides of a homopolymer can be resolved in pyrosequencing? FAQ ID -2871	浏览
	Can unused wells in a pyrosequencing plate be used in the next run? FAQ ID -2872	浏览
	What is the reason for signals ceasing in the middle of a pyrosequencing run? FAQ ID -2875	浏览
	How do I reduce background peaks in the pyrosequencing pyrogram? FAQ ID -2877	浏览
	How do I prevent a drifting baseline in my pyrosequencing pyrogram? FAQ ID -2878	浏览
	What is the reason for a high substrate peak in the pyrosequencing pyrogram? FAQ ID -2879	浏览
	What is the reason for split peaks appearing in between dispensations on my pyrosequencing pyrogram? FAQ ID -2881	浏览

学术海报

分类选项按字母顺序排序
	(EN) - Pyrosequencing and its applications	显示更多

试剂盒操作手册

分类选项按字母顺序排序
	(EN) - PyroMark APOE Handbook	显示更多
	(EN) - PyroMark HFE Handbook	显示更多
	(EN) - PyroMark MTHFR Handbook	显示更多
	(EN) - PyroMark Q96 CpG LINE-1 Handbook	显示更多

产品介绍与指南

分类选项按字母顺序排序
	Pyrosequencing – the synergy of sequencing and quantification	显示更多

安全数据表

分类选项按字母顺序排序
	Safety Data Sheets Download Safety Data Sheets for QIAGEN product components.	浏览

安全数据表

分类选项按字母顺序排序
	MSDS PyroMark APOE (96)
	MSDS PyroMark HFE (96)
	MSDS PyroMark MTHFR (96)
	MSDS PyroMark Q96 CpG LINE-1 (2 x 96)
	MSDS PyroMark Q96 CpG MGMT (96)
	MSDS PyroMark Q96 CpG MLH1 (96)
	MSDS PyroMark Q96 CpG p16 (96)
	MSDS PyroMark Q96 CpG Prader-Willi (96)

图片

PyroMark p16分析。

对[A]正常的血液DNA样本和[B]从结肠肿瘤中纯化的DNA样本进行分析。蓝色柱表示检测的CpG位点，以及每个位点的甲基化程度。黄色柱表示完全亚硫酸氢盐转化的质控。