QIAquick PCR Purification Kit

纯化多至10 μg PCR产物,100 bp–10 kb

  • 即用型DNA回收率高达95%
  • 3个简单步骤即可回收多达10 kb DNA片段
  • 含有凝胶上样染料,方便样本分析

QIAquick PCR Purification Kit包含离心柱、缓冲液和收集管,利用硅胶模技术纯化100 bp以上的PCR产物。 通过简单快速的结合、洗涤、洗脱步骤,使用30–50 μl洗脱体积即可纯化多达10 kb的DNA片段。内置的pH指示剂可方便测定DNA与离心柱结合的最佳pH值,整个过程可在QIAcube全自动核酸纯化仪上自动化运行。

Product Cat. no. List price:
QIAquick PCR Purification Kit (50)
For purification of 50 PCR reactions: 50 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)
28104
124,00 €
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QIAquick PCR Purification Kit (250)
For purification of 250 PCR reactions: 250 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)
28106
566,00 €
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QIAquick PCR Purification Kit (1000)
For purification of 1000 PCR reactions: 1000 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)
28106X4
1.784,00 €
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QIAquick Spin Columns (100)
For DNA fragment purification from PCR, gel fragments and enzymatic reactions
28115
124,00 €
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QIAquick PCR & Gel Cleanup Kit (100)
For purification PCR fragments and gel extractions of 100 reactions (at least 80–100 gel reactions or 100 PCRs)
28506
249,00 €
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The QIAquick PCR Purification Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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性能
QIAquick PCR Purification操作流程去除引物、核苷酸、酶、矿物油、盐和DNA样本中的其他杂质(参见"Complete primer removal after PCR")。通过使用微型离心机或者真空底座,可以纯化得到100 bp–10 kb的DNA片段。
原理

QIAquick Kits配备有硅胶膜,在高盐度缓冲液中结合DNA,应用低盐度缓冲液或水洗脱DNA。纯化过程去除引物、核苷酸、酶、矿物油、琼脂糖、溴化乙锭和DNA样本中的其他杂质(参见"Complete primer removal after PCR")。硅胶膜技术避免了松散树脂常见的问题和不便。特制的结合缓冲液可促进吸附特定片段范围内的DNA分子。 

凝胶上样染料

为更快、更方便地处理和分析样本,已提供凝胶上样染料。GelPilot Loading Dye含有3种示踪染料(二甲苯蓝、溴酚蓝和orange G),便于优化跑胶时间,避免小片段DNA跑得过远(参见"GelPilot Loading Dye")。

操作流程

QIAquick体系采用了简单的结合、洗涤、洗脱的流程(参见"QIAquick and MinElute procedure")。结合缓冲溶液可直接加入到PCR样本或者其他酶试剂中,然后将混合液上样到QIAquick离心柱。结合缓冲液含有pH值指示剂,可方便地确定DNA结合的最佳pH值(参见"pH Indicator Dye")。核酸在高盐度条件下结合到硅胶膜上,洗去杂质,使用小体积的低盐缓冲液或水洗脱纯DNA,得到的DNA可直接用于后续应用。 

处理过程

QIAquick 离心柱提供两个方便的操作方法。离心柱可放在传统的台式微型离心机上或通过适配器连接到任何真空底座上,如QIAvac 24 Plus和QIAvac Luer Adapters。QIAquick PCR Purification Kit以及其他的QIAGEN离心柱试剂盒可在QIAcube全自动核酸纯化仪器上全自动化运行,确保高产量和标准化的结果(参见"Spin column handling options A, B, C, D, and E")。

应用

QIAquick系统纯化的DNA片段可直接用于各种应用,包括测序、微阵列分析、连接和转化、限制性酶切、标记、显微注射、PCR和体外转录。

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User-Developed Protocols
1
The protocol has been used successfully for Cy3-, Cy5-, and biotin-labeling of cDNA from <50 ng of total RNA or poly A+ mRNA.
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Safety Data Sheets
1
Download Safety Data Sheets for QIAGEN product components.
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Protocol Files
2
QIAquick PCR Purification protocol

This protocol is for purification of single- or double-stranded DNA fragments (100 bp to 10 kb) from amplification reactions using the QIAquick PCR Purification Kit.

Sample Size 5-100 µl PCR samples
Elution volume 30-100 µl in increments of 10 µl, default 50µl
Applications Cleanup, DNA
Starting material Amplification reactions

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Purification of PCR products from 100-200 µl PCR samples

This protocol is for purification of double-stranded DNA fragments (100 bp to 10 kb) from PCR reactions of up to 200 µl sample volume (e.g., pooled samples) using the QIAquick PCR Purification Kit.

Sample Size 100-200 µl PCR samples
Elution volume 30-100 µl in increments of 10 µl, default 50µl
Applications Cleanup, DNA
Starting material Amplification reactions

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QIAcube Classic
2
QIAquick PCR Purification protocol

This protocol is for purification of single- or double-stranded DNA fragments (100 bp to 10 kb) from amplification reactions using the QIAquick PCR Purification Kit.

Sample Size 5-100 µl PCR samples
Elution volume 30-100 µl in increments of 10 µl, default 50µl
Applications Cleanup, DNA
Starting material Amplification reactions

Show details
Purification of PCR products from 100-200 µl PCR samples

This protocol is for purification of double-stranded DNA fragments (100 bp to 10 kb) from PCR reactions of up to 200 µl sample volume (e.g., pooled samples) using the QIAquick PCR Purification Kit.

Sample Size 100-200 µl PCR samples
Elution volume 30-100 µl in increments of 10 µl, default 50µl
Applications Cleanup, DNA
Starting material Amplification reactions

Show details
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