QIAamp MinElute Virus Spin Kit
从血浆、血清和无细胞体液中同时纯化病毒DNA和RNA
- 快速纯化高品质病毒DNA和RNA
- 无需有机提取或乙醇沉淀
- 重复性好,产量高
- 完全去除污染物和抑制剂
QIAamp MinElute Virus Spin Kit使用快速离心柱简化了纯化病毒DNA和RNA的操作流程。QIAamp MinElute Virus Spin Kit的起始样品体积可多至0.2 ml,使用选择性结合的硅胶膜,具有20–150 μl的灵活洗脱体积。QIAamp MinElute Virus Spin可在QIAcube全自动核酸纯化仪上全自动运行。
zh-CN
Configure
Added to your shopping cart
|
产品
|
Product no.
|
货号
|
目录价:
|
|
|
此产品已经停产。
我们推荐您使用
|
|
Show details
|
|
|
varies
|
select
Can't order online?
To place an order via phone, email or for requesting a quote, please provide the product’s name, number and catalog number.
|
|
|
|
产品
|
货号
|
目录价:
|
|
|
QIAamp MinElute Virus Spin Kit (50)
For 50 minipreps: 50 QIAamp MinElute Columns, QIAGEN Protease, carrier RNA, buffers, Collection Tubes (2 ml)
|
57704
|
|
|
QIAamp MinElute Virus Spin Kit适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。
QIAamp MinElute Virus Spin实验流程。|High sensitivity in PCR.|RT-PCR的高灵敏度。|
|The percentage of positive samples at low virus titers is shown. Nucleic acids from a DNA virus were purified from plasma using either the QIAamp MinElute Spin or Vacuum Kit and subjected to PCR. For the spin procedure, 95% probit values for 40 samples were 27.25 IU/ml and for the vacuum procedure, they were 9.30 IU/ml.|低病毒滴度的阳性样本百分比如图所示。使用QIAamp MinElute Spin或Vacuum Kit从血浆中纯化RNA病毒核酸,并进行RT-PCR。使用离心柱步骤纯化的32个样本的95%概率值为22.84 IU/ml,使用真空步骤的95%概率值为9.46 IU/ml。(数据由QIAGEN R&D部门生成。)|
原理
QIAamp MinElute Virus Spin Kit使用快速离心柱简化了从血浆、血清和无细胞体液中分离病毒RNA和DNA的过程。无需苯酚-氯仿抽提。核酸特异性结合到QIAamp MinElute硅胶膜上,污染物流出。通过两步高效洗涤可完全去除二价阳离子和蛋白质等PCR抑制剂,再用水或试剂盒中的缓冲液洗脱结合在离心柱上的纯核酸。QIAamp MinElute技术从血浆、血清和无细胞体液中纯化得到病毒RNA和DNA,可即时用于PCR和印迹实验中。
QIAamp MinElute Virus Spin Kit同时具备硅胶膜选择结合DNA的特性,以及20–150 µl的灵活洗脱体积。操作流程适用于血浆、血清和无细胞体液。QIAamp样本制备技术完全得到验证认可。
操作流程
试剂盒提供优化的缓冲液高效裂解样本,稳定核酸并促进DNA选择性结合到QIAamp MinElute硅胶膜上(参见" QIAamp MinElute Virus Spin procedure")。将添加乙醇的裂解液上样到QIAamp MinElute离心柱上。洗涤缓冲液用于去除杂质,用Buffer AVE洗脱的病毒核酸,可即时用于扩增反应或储存于–20ºC。纯化的核酸不含蛋白质、核酸酶或其他杂质。
应用
QIAamp MinElute Virus Spin Kit使用成熟技术,可从多种样本中同时纯化病毒RNA和DNA,包括:
- 新鲜或冷冻的血浆
- 血清
- 其他无细胞体液
|
特点
|
参数
|
|
应用
|
PCR, real-time PCR
|
|
洗脱体积
|
20–150 µl
|
|
规格
|
MinElute columns
|
|
主要样本类型
|
Serum, plasma
|
|
处理
|
Manual (centrifugation)
|
|
纯化总RNA、miRNA、poly A+ mRNA、DNA或蛋白
|
Viral DNA, viral RNA
|
|
样本量
|
200 µl
|
|
技术
|
Silica technology
|
|
每次运行或制备时间
|
<1 hour
|
|
产量
|
Varies
|
|
|
|
|
FAQ ID -12
|
浏览
|
|
|
FAQ ID -295
|
浏览
|
|
|
I received one of the kits in the list below containing the MinElute columns, however they were left out for a while and not stored at 2-8°C upon receipt. Can I still use them? AllPrep DNA/RNA Micro, EpiTect Fast DNA Bisulfite, EpiTect Fast FFPE Bisulfite, EpiTect Fast LyseAll Bisulfite, EpiTect Plus DNA Bisulfite, EpiTect Plus FFPE Bisulfite, EpiTect Plus LyseAll Bisulfite, exoRNeasy Serum/plasma Maxi, exoRNeasy Serum/Plasma Midi, GeneRead DNA FFPE, GeneRead rRNA Depletion, GeneRead Size Selection, MinElute Gel Extraction, MinElute PCR Purification, MinElute Reaction Cleanup, miRNeasy FFPE, miRNeasy Micro, miRNeasy Serum/Plasma, QIAamp DNA FFPE, QIAamp DNA Investigator, QIAamp DNA Micro, QIAamp MinElute Media, QIAamp MinElute Virus Spin, QIAamp MinElute Virus Vacuum, RNeasy FFPE, RNeasy Micro, RNeasy Plus Micro
FAQ ID - 3560
|
浏览
|
|
|
FAQ ID -472
|
浏览
|
|
|
FAQ ID -528
|
浏览
|
|
|
FAQ ID -635
|
浏览
|
|
|
FAQ ID -728
|
浏览
|
|
|
FAQ ID -761
|
浏览
|
|
|
FAQ ID -351
|
浏览
|
图片
QIAamp MinElute Virus Spin实验流程。
High sensitivity in PCR.
The percentage of positive samples at low virus titers is shown. Nucleic acids from a DNA virus were purified from plasma using either the QIAamp MinElute Spin or Vacuum Kit and subjected to PCR. For the spin procedure, 95% probit values for 40 samples were 27.25 IU/ml and for the vacuum procedure, they were 9.30 IU/ml.
RT-PCR的高灵敏度。
低病毒滴度的阳性样本百分比如图所示。使用QIAamp MinElute Spin或Vacuum Kit从血浆中纯化RNA病毒核酸,并进行RT-PCR。使用离心柱步骤纯化的32个样本的95%概率值为22.84 IU/ml,使用真空步骤的95%概率值为9.46 IU/ml。(数据由QIAGEN R&D部门生成。)
|