用于配合PyroMark Q96 ID和PyroMark Q96 MD进行基因特异的序列变异和甲基化

  • 检测并定量分析癌症相关基因的突变
  • 定量分析不同基因的单个或多个CpG位点
  • 确定特定区域的CpG位点甲基化状况
  • 序列背景提供检测的内置参照
PyroMark Q96 Tests适用于高度可靠的突变及SNP定量检测和CpG位点甲基化分析,经优化配合PyroMark Q96 MD和/或PyroMark Q96 ID使用。产品名带有“Q96”的产品应与PyroMark Q96 MD和/或PyroMark Q96 ID仪器配套使用,PyroMark HFE和PyroMark MTHFR等其他产品也可配合PyroMark Q24使用(PyroMark APOE除外)。
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PyroMark APOE (96)
For detection of polymorphism in the APOE gene (2 x 96 reactions on PyroMark Q96 MD or 1 x 96 reactions on PyroMark Q96 ID)
972422
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PyroMark HFE (96)
For detection of variants in the HFE gene (2 x 96 reactions on PyroMark Q96 MD, 1 x 96 reactions on PyroMark Q96 ID, 8 x 24 reactions on PyroMark Q24)
972442
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PyroMark MTHFR (96)
For detection of variants in the MTHFR gene (2 x 96 reactions on PyroMark Q96 MD, 1 x 96 reactions on PyroMark Q96 ID, 8 x 24 reactions on PyroMark Q24)
972432
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PyroMark Q96 CpG LINE-1 (2 x 96)
For quantification of global methylation level in transposable elements; only for use with PyroMark Q96 MD
973043
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PyroMark Q96 CpG MGMT (96)
For quantification of methylation level in the MGMT gene (2 x 96 reactions on PyroMark Q96 MD and 1 x 96 reactions on PyroMark Q96 ID)
972032
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PyroMark Q96 CpG MLH1 (96)
For quantification of methylation level in promoter region of the MLH1 gene (2 x 96 reactions on PyroMark Q96 MD and 1 x 96 reactions on PyroMark Q96 ID)
972022
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PyroMark Q96 CpG p16 (96)
For quantification of methylation level in the p16 gene (2 x 96 reactions on PyroMark Q96 MD and 1 x 96 reactions on PyroMark Q96 ID)
972012
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PyroMark Q96 CpG Prader-Willi (96)
For quantification of methylation level in Prader-Willi and Angelman Syndromes (2 x 96 reactions on PyroMark Q96 MD and 1 x 96 reactions on PyroMark Q96 ID)
972052
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PyroMark APOE、PyroMark HFE、PyroMark MTHFR、PyroMark Q96 CpG LINE-1、PyroMark Q96 CpG MGMT、PyroMark
Q96 CpG MLH1、PyroMark Q96 CpG p16和PyroMark Q96 CpG Prader-Willi检测适用于分子生物学应用,不适用于疾病的诊断、预防或治疗。
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PyroMark p16 analysis.|
[A] A normal blood DNA sample and [B] a DNA sample isolated from a colon tumor were analyzed. Blue columns indicate the measured CpG sites, and the individually measured degree of methylation at each site. Yellow columns indicate QC for completion of the bisulfite treatment.|
Performance
PyroMark Q96 Tests适合用于准确检测CpG的甲基化状态,例如PyroMark Q96 CpG p16 Test(参见“PyroMark p16 analysis”)。
Principle

PyroMark Q96 Tests配合PyroMark Q96 MD和/或PyroMark Q96 ID Systems采用焦磷酸测序技术准确定量突变(或SNP)和进行甲基化分析。这些产品包括PCR引物和一个测序引物。

Procedure

在焦磷酸测序之前,形成了一个生物素标记的PCR产物,该产物结合到覆盖链酶亲和素的琼脂糖凝胶珠上。这些胶珠会被真空仪器捕捉并彻底清洗,随后变性,得到适合测序的单链DNA。PyroMark Q96 Tests提供预制的甲基化分析试剂,用于焦磷酸测序。每个分析含有用于扩增生物素标记的PCR产物所需的引物,也含有焦磷酸测序反应所需的引物。

Applications

PyroMark Q96 Tests能够高度准确的定量检测甲基化和进行突变(或SNP)分析,用于多个研究领域,包括癌症和表观遗传学研究。

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