CRISPR/CAS9 Gene Editing

Crispr
Design experiment
Prepare sgRNA expression plasmid
Transfect target cells
Validate gene editing
Step 1: Design experiment
2
Ingenuity Variant Analysis
For identifying disease-causing variants from human whole genome, exome and gene panel next-generation sequencing studies
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Ingenuity Pathway Analysis (IPA)
For modeling, analyzing, and understanding complex 'omics data
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Step 2: Prepare sgRNA expression plasmid
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QIAprep Spin Miniprep Kit
For purification of up to 20 μg molecular biology grade plasmid DNA
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QIAquick Gel Extraction Kit
For gel extraction/cleanup of up to 10 μg DNA (70 bp to 10 kb) from enzymatic reactions
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QIAquick PCR Purification Kit
For purification of up to 10 μg PCR products, 100 bp to 10 kb
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HotStar HiFidelity Polymerase Kit
For highly sensitive and reliable high-fidelity hot-start PCR
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QIAxcel Advanced System
For effortless DNA and RNA analysis
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QIAcube
For fully automated sample prep using QIAGEN spin-column kits
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QIAcube HT
Instrument for automated mid- to high-throughput nucleic acid purification in 96-well format
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Step 3: Transfect target cells
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Attractene Transfection Reagent
For efficient DNA transfection and DNA–siRNA/miRNA cotransfection
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Effectene Transfection Reagent
For DNA transfection of primary cells and sensitive cell lines
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QIAGEN Plasmid Plus 96 Kits
For purification of up to 50 µg transfection-grade plasmid DNA in 96-well format
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QIAGEN Plasmid Plus Kits
For the fastest, most convenient purification of up to 10 mg transfection-grade plasmid DNA
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HiSpeed Plasmid Kits
For ultrafast purification of up to 750 µg transfection-grade plasmid or cosmid DNA
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EndoFree Plasmid Kits
For purification of up to 10 mg endotoxin-free advanced transfection-grade plasmid or cosmid DNA
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QIAprep Spin Miniprep Kit
For purification of up to 20 μg molecular biology grade plasmid DNA
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QIAcube
For fully automated sample prep using QIAGEN spin-column kits
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QIAcube HT
Instrument for automated mid- to high-throughput nucleic acid purification in 96-well format
Show details
See products
Show all products
Step 4: Validate gene editing
10
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HotStar HiFidelity Polymerase Kit
For highly sensitive and reliable high-fidelity hot-start PCR
Show details
See products
QIAquick PCR Purification Kit
For purification of up to 10 μg PCR products, 100 bp to 10 kb
Show details
See products
QIAprep Spin Miniprep Kit
For purification of up to 20 μg molecular biology grade plasmid DNA
Show details
See products
QIAxcel Advanced System
For effortless DNA and RNA analysis
Show details
See products
QIAcube
For fully automated sample prep using QIAGEN spin-column kits
Show details
See products
QIAcube HT
Instrument for automated mid- to high-throughput nucleic acid purification in 96-well format
Show details
See products
PyroMark Q48 Autoprep
For automated Pyrosequencing with integrated template preparation for advanced methylation, mutation and SNP quantification
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Ingenuity Variant Analysis
For identifying disease-causing variants from human whole genome, exome and gene panel next-generation sequencing studies
Show details
See products
Ingenuity Pathway Analysis (IPA)
For modeling, analyzing, and understanding complex 'omics data
Show details
See products
RT2 Profiler PCR Arrays
For pathway-focused gene expression analysis using laboratory-verified assays
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Sample to Insight
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